Invitrogen

HELIOS Monoclonal Antibody (22F6), PE-eFluor™ 610, eBioscience™

HELIOS Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

HELIOS Antibody (61-9883-42) in Flow

Intracellular staining of mouse lymph node cells with Anti-Mouse/Rat Foxp3 eFluor® 450 (Product # 48-5773-82) and Armenian Hamster IgG Isotype Control PE-eFluor® 610 (Product # 61-4888-82) (left) or Anti-Helios PE-eFluor® 610 (right) using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. CD4+ (Product # 17-0042-82) cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

61-9883-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Rhesus monkey

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

22F6

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574682

Product Specific Information

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.

22F6 has been reported to cross react with rat Helios.

Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse lymph node cells using the using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Activation-induced cytidine deaminase expression by thymic B cells promotes T-cell tolerance and limits autoimmunity.

iScience

Lombard-Vadnais F,Chabot-Roy G,Zahn A,Rodriguez Torres S,Di Noia JM,Melichar HJ,Lesage S

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Fri Jan 20 00:00:00 EST 2023

Interaction between tissue-dwelling helminth and the gut microbiota drives mucosal immunoregulation.

NPJ biofilms and microbiomes

Wang Y,Guo A,Zou Y,Mu W,Zhang S,Shi Z,Liu Z,Cai X,Zhu XQ,Wang S

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Sat Jun 24 00:00:00 EDT 2023

Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.

Nature communications

Piekarska K,Urban-Wójciuk Z,Kurkowiak M,Pelikant-Małecka I,Schumacher A,Sakowska J,Spodnik JH,Arcimowicz Ł,Zielińska H,Tymoniuk B,Renkielska A,Siebert J,Słomińska E,Trzonkowski P,Hupp T,Marek-Trzonkowska NM

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Mon Feb 14 00:00:00 EST 2022

CRISPR/Cas9-Mediated Insertion of HIV Long Terminal Repeat within BACH2 Promotes Expansion of T Regulatory-like Cells.

Journal of immunology (Baltimore, Md. : 1950)

Christian ML,Dapp MJ,Scharffenberger SC,Jones H,Song C,Frenkel LM,Krumm A,Mullins JI,Rawlings DJ

61-9883-42 was used in Flow cytometry/Cell sorting to model the effects of HIV integration within BACH2 Integration of the HIV long terminal repeat (LTR) and major splice donor increased BACH2 mRNA and protein levels, altered gene expression, and promoted selective outgrowth of an activated, proliferative, and T regulatory-like cell population.

Fri Apr 01 00:00:00 EDT 2022

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells.

Frontiers in immunology

Ramos-Ramírez P,Malmhäll C,Tliba O,Rådinger M,Bossios A

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Tue Oct 26 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

Published figure using HELIOS monoclonal antibody (Product # 61-9883-42) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.

Cell reports

Shah SV,Manickam C,Ram DR,Kroll K,Itell H,Permar SR,Barouch DH,Klatt NR,Reeves RK

61-9883-42 was used in Flow Cytometry to provide a mechanism of virus-dependent alternative signaling that may explain the acquisition of adaptive features by primate NK cells and could be targeted for future vaccine or curative therapies.

Tue Dec 04 00:00:00 EST 2018

Bioinformatics

Protein Aliases: IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios)

View more View less

Gene Aliases: A730095J18Rik; ANF1A2; HELIOS; IKZF2; Zfpn1a2; ZNF1A2; ZNFN1A2

View more View less

UniProt ID: (Human) Q9UKS7, (Mouse) P81183

View more View less

Entrez Gene ID: (Dog) 488510, (Human) 22807, (Mouse) 22779

View more View less

Function(s)

molecular_function transcription factor activity, sequence-specific DNA binding protein binding protein homodimerization activity transcription regulatory region DNA binding metal ion binding protein heterodimerization activity nucleic acid binding DNA binding C2H2 zinc finger transcription factor

Process(es)

transcription, DNA-templated biological_process positive regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HELIOS Monoclonal Antibody (22F6), PE-eFluor™ 610, eBioscience™

HELIOS Antibody (61-9883-42) in Flow

Product Details

61-9883-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!