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HES1 Antibody (PA5-13268) in ICC/IF
Immunocytochemistry analysis of HES1 in HeLa cells. Samples were incubated in HES1 polyclonal antibody (Product # PA5-13268) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Product Details
PA5-13268
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human HES1
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2117849
Product Specific Information
This antibody is predicted to react with bovine, mouse, rat and Xenopus based on sequence homology.
Target Information
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb39; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; Hairy homolog; Hairy-like; Hairy-like protein; hHL; Transcription factor HES-1
Gene Aliases: BHLHB39; HES-1; HES1; HHL; HL; HRY
UniProt ID: (Human) Q14469
Entrez Gene ID: (Human) 3280
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
chaperone binding
N-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
liver development
embryonic heart tube morphogenesis
outflow tract morphogenesis
regulation of secondary heart field cardioblast proliferation
ventricular septum development
transcription, DNA-templated
protein complex assembly
cell adhesion
Notch signaling pathway
smoothened signaling pathway
STAT protein import into nucleus
nervous system development
positive regulation of cell proliferation
cell migration
telencephalon development
midbrain-hindbrain boundary morphogenesis
oculomotor nerve development
trochlear nerve development
hindbrain morphogenesis
forebrain radial glial cell differentiation
adenohypophysis development
lung development
positive regulation of BMP signaling pathway
midbrain development
pancreas development
somatic stem cell population maintenance
ascending aorta morphogenesis
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
auditory receptor cell fate determination
positive regulation of DNA binding
regulation of fat cell differentiation
negative regulation of auditory receptor cell differentiation
positive regulation of Notch signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of mitotic cell cycle, embryonic
lateral inhibition
positive regulation of JAK-STAT cascade
cell maturation
thymus development
cell morphogenesis involved in neuron differentiation
positive regulation of astrocyte differentiation
negative regulation of oligodendrocyte differentiation
artery morphogenesis
regulation of epithelial cell proliferation
inner ear receptor stereocilium organization
regulation of timing of neuron differentiation
negative regulation of glial cell proliferation
ventricular septum morphogenesis
ureteric bud morphogenesis
labyrinthine layer blood vessel development
common bile duct development
negative regulation of stomach neuroendocrine cell differentiation
cardiac neural crest cell development involved in outflow tract morphogenesis
pharyngeal arch artery morphogenesis
glomerulus vasculature development
comma-shaped body morphogenesis
S-shaped body morphogenesis
renal interstitial fibroblast development
metanephric nephron tubule morphogenesis
cochlea development
establishment of epithelial cell polarity
vascular smooth muscle cell development
neuronal stem cell population maintenance
positive regulation of protein targeting to mitochondrion
negative regulation of pancreatic A cell differentiation
negative regulation of stem cell differentiation
negative regulation of pro-B cell differentiation
negative regulation of forebrain neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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