Invitrogen

HES1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (6)
Published Figures (11)

HES1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

HES1 Antibody (PA5-28802) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of HES1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 Polyclonal Antibody (Product # PA5-28802) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

HES1 Antibody in Immunoprecipitation (IP)

HES1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HES1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

HES1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-28802

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100-1:2,000

View 3 publications 3 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 45 of Human HES1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546278

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse kidney, PC-12.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus.

iScience

O'Brien BS,Mokry RL,Schumacher ML,Pulakanti K,Rao S,Terhune SS,Ebert AD

Published figure using HES1 polyclonal antibody (Product # PA5-28802) in Immunocytochemistry

Fri Apr 15 00:00:00 EDT 2022

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma.

Cell death & disease

Islam SS,Qassem K,Islam S,Parag RR,Rahman MZ,Farhat WA,Yeger H,Aboussekhra A,Karakas B,Noman ASM

PA5-28802 was used in Western Blotting to suggest that Keap1 mutations define a distinct disease phenotype and the Keap1-Nrf2 pathway is one of the leading molecular mechanisms for clinical chemotherapeutic resistance.

Tue Aug 09 00:00:00 EDT 2022

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma.

Cell death & disease

Islam SS,Qassem K,Islam S,Parag RR,Rahman MZ,Farhat WA,Yeger H,Aboussekhra A,Karakas B,Noman ASM

Tue Aug 09 00:00:00 EDT 2022

Genetic alterations of Keap1 confers chemotherapeutic resistance through functional activation of Nrf2 and Notch pathway in head and neck squamous cell carcinoma.

Cell death & disease

Islam SS,Qassem K,Islam S,Parag RR,Rahman MZ,Farhat WA,Yeger H,Aboussekhra A,Karakas B,Noman ASM

Tue Aug 09 00:00:00 EDT 2022

TGF-β3 induces lactate production in Sertoli cell through inhibiting the Notch pathway.

Andrology

Xu Y,Jiang S,Hu Y,Zhang Q,Su W

Published figure using HES1 polyclonal antibody (Product # PA5-28802) in Western Blot

Tue Nov 01 00:00:00 EDT 2022

TGF-β3 induces lactate production in Sertoli cell through inhibiting the Notch pathway.

Andrology

Xu Y,Jiang S,Hu Y,Zhang Q,Su W

Published figure using HES1 polyclonal antibody (Product # PA5-28802) in Western Blot

Tue Nov 01 00:00:00 EDT 2022

Rutin Potentially Binds the Gamma Secretase Catalytic Site, Down Regulates the Notch Signaling Pathway and Reduces Sphere Formation in Colonospheres.

Metabolites

Singh AK,Shuaib M,Prajapati KS,Kumar S

PA5-28802 was used in Western Blotting to suggest that rutin inhibits the notch signaling pathway and reduces the stemness/self-renewal property in colon cancer cells and the colonospheres by targeting gamma secretase.

Thu Sep 29 00:00:00 EDT 2022

Aberrant expression of semaphorin 6B affects cell phenotypes in thyroid carcinoma by activating the Notch signalling pathway.

Endokrynologia Polska

Lv XJ,Chen X,Wang Y,Yu S,Pang L,Huang C

PA5-28802 was used in Western Blot to demonstrate that SEMA6B exerts a tumourigenic effect in thyroid carcinoma partly by activating Notch signalling pathway, which provides a possible biomarker for the therapeutic intervention in thyroid carcinoma.

Wed Oct 20 00:00:00 EDT 2021

Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes.