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Product Details
14-9799-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4H1HES1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10853019
Product Specific Information
Description: This 4H1HES monoclonal antibody reacts with human Hes1, a 30-kDa basic-helix-loop-helix transcriptional repressor. Hes1 is expressed in thymocytes, as well as neural, retinal, liver, and pancreatic tissues. Expression of Hes1 is controlled by Notch signaling, and thus plays an important role in T cell development. This regulation by Notch is also critical for neural stem cell differentiation and neurogenesis. Reports have demonstrated that Hes1 antagonizes transcription of MASH1, a basic-helix-loop-helix factor involved in neuronal determination. Finally, Hes1 has been associated with Notch-induced T cell acute lymphoblastic lymphoma and other cancers.
Applications Reported: This 4H1HES antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P).
Applications Tested: This 4H1HES antibody has been tested by immunohistochemistry on FFPE human kidney cortex at less than or equal to 20 µg/mL both without antigen retrieval or with high pH antigen retrieval. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb39; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; Hairy homolog; Hairy-like; Hairy-like protein; hHL; Transcription factor HES-1
Gene Aliases: BHLHB39; HES-1; HES1; HHL; HL; HRY
UniProt ID: (Human) Q14469
Entrez Gene ID: (Human) 3280
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
chaperone binding
N-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
liver development
embryonic heart tube morphogenesis
outflow tract morphogenesis
regulation of secondary heart field cardioblast proliferation
ventricular septum development
transcription, DNA-templated
protein complex assembly
cell adhesion
Notch signaling pathway
smoothened signaling pathway
STAT protein import into nucleus
nervous system development
positive regulation of cell proliferation
cell migration
telencephalon development
midbrain-hindbrain boundary morphogenesis
oculomotor nerve development
trochlear nerve development
hindbrain morphogenesis
forebrain radial glial cell differentiation
adenohypophysis development
lung development
positive regulation of BMP signaling pathway
midbrain development
pancreas development
somatic stem cell population maintenance
ascending aorta morphogenesis
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
auditory receptor cell fate determination
positive regulation of DNA binding
regulation of fat cell differentiation
negative regulation of auditory receptor cell differentiation
positive regulation of Notch signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of mitotic cell cycle, embryonic
lateral inhibition
positive regulation of JAK-STAT cascade
cell maturation
thymus development
cell morphogenesis involved in neuron differentiation
positive regulation of astrocyte differentiation
negative regulation of oligodendrocyte differentiation
artery morphogenesis
regulation of epithelial cell proliferation
inner ear receptor stereocilium organization
regulation of timing of neuron differentiation
negative regulation of glial cell proliferation
ventricular septum morphogenesis
ureteric bud morphogenesis
labyrinthine layer blood vessel development
common bile duct development
negative regulation of stomach neuroendocrine cell differentiation
cardiac neural crest cell development involved in outflow tract morphogenesis
pharyngeal arch artery morphogenesis
glomerulus vasculature development
comma-shaped body morphogenesis
S-shaped body morphogenesis
renal interstitial fibroblast development
metanephric nephron tubule morphogenesis
cochlea development
establishment of epithelial cell polarity
vascular smooth muscle cell development
neuronal stem cell population maintenance
positive regulation of protein targeting to mitochondrion
negative regulation of pancreatic A cell differentiation
negative regulation of stem cell differentiation
negative regulation of pro-B cell differentiation
negative regulation of forebrain neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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