Rockland

HEY1 Polyclonal Antibody

View all (23) HEY1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-HB5

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:100
-

ELISA (ELISA)

1:10,000-1:50,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-HEY1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human HEY1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.06 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human HEY1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and 91.7% with dog and cattle.

Target Information

Hairy/enhancer-of-split related with YRPW motif protein 1 belongs to the HEY family and is a Notch Signaling Pathway Protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. It is a downstream effector of Notch signaling which may be required for cardiovascular development. It acts as transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. It represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Hey1 is also reported to be expressed during development of the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: basic helix-loop-helix protein OAF1; bHLHb31; Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split-related protein 1; Hairy-related transcription factor 1; Hairy/E(spl)-related with YRPW motif 1; hairy/enhancer-of-split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; hes-related family bHLH transcription factor with YRPW motif 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HRT-1; MGC1274; mHRT1

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Gene Aliases: AI316788; AI414254; BHLHB31; CHF2; HERP2; hesr-1; HESR1; HEY1; hHRT1; HRT-1; HRT1; OAF1

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UniProt ID: (Human) Q9Y5J3

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Entrez Gene ID: (Human) 23462, (Mouse) 15213

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Function(s)
transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, protein binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding microsatellite binding protein dimerization activity transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding sequence-specific DNA binding basic helix-loop-helix transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis pulmonary valve morphogenesis atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation endocardial cushion morphogenesis cardiac ventricle morphogenesis transcription, DNA-templated Notch signaling pathway dorsal aorta morphogenesis umbilical cord morphogenesis negative regulation of Notch signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cardiac epithelial to mesenchymal transition heart trabecula formation cardiac septum morphogenesis ventricular septum morphogenesis labyrinthine layer blood vessel development arterial endothelial cell differentiation Notch signaling involved in heart development cellular response to glucocorticoid stimulus negative regulation of transcription regulatory region DNA binding negative regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation regulation of vasculogenesis blood vessel development vasculogenesis osteoblast development outflow tract morphogenesis atrioventricular valve morphogenesis endocardial cushion to mesenchymal transition involved in heart valve formation ventricular trabecula myocardium morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development anterior/posterior axis specification negative regulation of gene expression mesenchymal cell development negative regulation of neuron differentiation positive regulation of osteoblast differentiation artery development
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