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HEY1 Antibody (PA5-23484) in WB
Western blot analysis of HEY1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified antibody. Samples were incubated in HEY1 polyclonal antibody (Product # PA5-23484) followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Product Details
PA5-23484
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 50-100 of mouse Hey1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2540984
Target Information
Hairy/enhancer-of-split related with YRPW motif protein 1 belongs to the HEY family and is a Notch Signaling Pathway Protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. It is a downstream effector of Notch signaling which may be required for cardiovascular development. It acts as transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. It represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Hey1 is also reported to be expressed during development of the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hairy and enhancer of split-related protein 1; hairy-related transcription factor 1; Hairy/E(spl)-related with YRPW motif 1; hairy/enhancer-of-split related with YRPW motif protein 1; HRT-1; MGC1274; mHRT1
Gene Aliases: AI316788; AI414254; bHLHb31; CHF2; Herp2; hesr-1; Hesr1; HRT1
Entrez Gene ID: (Mouse) 15213
transcription factor activity, RNA polymerase II core promoter sequence-specific
transcription factor activity, protein binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
microsatellite binding
sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
vasculogenesis
osteoblast development
outflow tract morphogenesis
atrioventricular valve morphogenesis
pulmonary valve morphogenesis
atrioventricular valve formation
epithelial to mesenchymal transition involved in endocardial cushion formation
endocardial cushion to mesenchymal transition involved in heart valve formation
endocardial cushion morphogenesis
cardiac ventricle morphogenesis
ventricular trabecula myocardium morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Notch signaling pathway
multicellular organism development
anterior/posterior axis specification
negative regulation of gene expression
mesenchymal cell development
dorsal aorta morphogenesis
umbilical cord morphogenesis
negative regulation of neuron differentiation
positive regulation of osteoblast differentiation
negative regulation of Notch signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cardiac epithelial to mesenchymal transition
cardiac septum morphogenesis
ventricular septum morphogenesis
labyrinthine layer blood vessel development
artery development
arterial endothelial cell differentiation
Notch signaling involved in heart development
negative regulation of transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation
regulation of vasculogenesis
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