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HIV1 Reverse Transcriptase Antibody (PA1-4948) in WB
Detection of HIV-1 reverse transcriptase in the extract of HIV-1 infected cells by Western blotting using an HIV Reverse Transcriptase antibody (Product # PA1-4948). Lane 1: Extract of MT4 cells. Lane 2: Extract of MT4 cells infected with HIV-1 (LAI strain). The antiserum was diluted 2,500 fold before use.
Product Details
PA1-4948
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full size functional recombinant HIV 1 expressed and purified from E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_612274
Product Specific Information
PA1-4948 detects the HIV-1 Reverse Transcriptase protein in HIV samples.
PA1-4948 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~66 kDa protein and a ~51 kDa protein, corresponding respectively to the two subunits of HIV-1 reverse transcriptase, in HIV samples.
PA1-4948 immunogen corresponds to the HIV-1 Reverse Transcriptase which was over-expressed in E. coli as a recombinant protein.
Recombinant HIV-1 Reverse Transcriptase protein (Cat. # RP-4912 or RP-4913) can be purchased for control experiments in Western blot assays.
Target Information
HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1(AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HIV RT
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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