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HIV1 nef Antibody (PA1-4963) in WB
Detection of HIV-1 Nef by Western blotting using an HIV1 Nef Polyclonal Antibody (Product # PA1-4963). Lane1: Extract of MT4 cells. Lane2: Extract of MT4 cells infected with HIV-1 (LAI strain). The antiserum was diluted 1:1,000. Nef protein band is identified at 27 kDa.
Product Details
PA1-4963
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Guinea pig
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified full-size recombinant Nef of HIV1 group M, subtype B expressed in E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_612267
Product Specific Information
PA1-4963 detects the HIV-1 Nef protein in HIV samples.
PA1-4963 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~27 kDa protein, corresponding to HIV-1 Nef in HIV samples.
PA1-4963 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the Nef coding region of the HIV-1 virus.
Recombinant HIV-1 Nef protein (Cat. # RP-4922 or RP-4923) can be purchased for control experiments in Western blot assays.
Target Information
HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of receptor protein of the cell surface such as CD 4 and MH4, important for AIDS virus infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HIV-1 Nef
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