Invitrogen

HIV1 nef Monoclonal Antibody (2H12)

FIGURE: 1 / 10

HIV1 nef Antibody (MA1-71505) in WB

Western blot analysis of recombinant HIV-1 Nef, B subtype (lane 1) and a protein ladder (lane 2) using a HIV1 nef monoclonal antibody (Product # MA1-71505).

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Product Details

MA1-71505

Applications

Tested Dilution

Publications

Western Blot (WB)

0.8 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.027-0.8 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2H12

Immunogen

Recombinant Nef protein of HIV-1 BRU purified from E. coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_962107

Product Specific Information

This antibody reacts with HIV-1 Nef, B and C subtypes, BRU, IIIB, and HAN strains.

This clone may also be referred to as 2H12 (01-007).

Target Information

HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of receptor protein of the cell surface such as CD 4 and MH4, important for AIDS virus infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner.

Autophagy

Castro-Gonzalez S,Shi Y,Colomer-Lluch M,Song Y,Mowery K,Almodovar S,Bansal A,Kirchhoff F,Sparrer K,Liang C,Serra-Moreno R

MA1-71505 was used in Western Blot to demonstrate that HIV-1 is susceptible to autophagy restriction and define Nef as the primary autophagy antagonist of this antiviral process.

Mon Feb 01 00:00:00 EST 2021

HIV-1 Nef counteracts autophagy restriction by enhancing the association between BECN1 and its inhibitor BCL2 in a PRKN-dependent manner.

Autophagy

Castro-Gonzalez S,Shi Y,Colomer-Lluch M,Song Y,Mowery K,Almodovar S,Bansal A,Kirchhoff F,Sparrer K,Liang C,Serra-Moreno R

MA1-71505 was used in Western Blot to demonstrate that HIV-1 is susceptible to autophagy restriction and define Nef as the primary autophagy antagonist of this antiviral process.

Mon Feb 01 00:00:00 EST 2021

Residues T₄₈ and A₄₉ in HIV-1 NL4-3 Nef are responsible for the counteraction of autophagy initiation, which prevents the ubiquitin-dependent degradation of Gag through autophagosomes.

Retrovirology

Castro-Gonzalez S,Chen Y,Benjamin J,Shi Y,Serra-Moreno R

MA1-71505 was used in Immunoprecipitation to uncover details of the mutual antagonism between HIV-1 and autophagy and uncover the genetic determinants that drive the autophagy-mediated restriction of HIV-1 as well as the counteraction imposed by Nef.

Thu Oct 28 00:00:00 EDT 2021

Residues T₄₈ and A₄₉ in HIV-1 NL4-3 Nef are responsible for the counteraction of autophagy initiation, which prevents the ubiquitin-dependent degradation of Gag through autophagosomes.

Retrovirology

Castro-Gonzalez S,Chen Y,Benjamin J,Shi Y,Serra-Moreno R

Thu Oct 28 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: HIV-1 Nef

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HIV1 nef Monoclonal Antibody (2H12)

HIV1 nef Antibody (MA1-71505) in WB

Product Details

MA1-71505

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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