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HIV1 p17 Antibody (PA1-4954) in WB
Detection of HIV-1 p17 and its p55 precursor protein by Western blotting using an HIV1 p17 Polyclonal Antibody (Product # PA1-4951). Lane 1: Extract of MT4 cells. Lane 2: Extract of MT4 cells infected with HIV-1 (LAI strain). The antiserum was diluted 1:2,500.
Product Details
PA1-4954
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified full-size recombinant Gag p17 of HIV1 subtype B expressed in E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_612265
Product Specific Information
PA1-4954 detects the HIV-1 p17 protein in HIV samples.
PA1-4954 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~17 kDa protein, and a ~55 kDa protein, corresponding respectively to HIV-p17 and to its precursor p55, in HIV samples.
PA1-4954 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the Gag p17 coding region of the HIV-1 virus.
Recombinant HIV-1 Gag p17 protein (Cat. # RP-4914 or RP-4915) can be purchased for control experiments in Western blot assays.
Target Information
HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the of AIDS virus particle construction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HIV1gp1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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