Proteintech

HK2 Monoclonal Antibody (2A11C3)

HK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HK2 Antibody (66974-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

66974-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A11C3

Immunogen

HK2 Fusion Protein Ag16895

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The regulation of cell homeostasis and antiviral innate immunity by autophagy during classical swine fever virus infection.

Emerging microbes & infections

Li X,Song Y,Wang X,Fu C,Zhao F,Zou L,Wu K,Chen W,Li Z,Fan J,Li Y,Li B,Zeng S,Liu X,Zhao M,Yi L,Chen J,Fan S

Fri Dec 01 00:00:00 EST 2023

Rare sugar L-sorbose exerts antitumor activity by impairing glucose metabolism.

Communications biology

Xu HL,Zhou X,Chen S,Xu S,Li Z,Nakanishi H,Gao XD

Sat Mar 11 00:00:00 EST 2023

Ectopic endometriotic stromal cells-derived lactate induces M2 macrophage polarization via Mettl3/Trib1/ERK/STAT3 signalling pathway in endometriosis.

Immunology

Gou Y,Wang H,Wang T,Wang H,Wang B,Jiao N,Yu Y,Cao Y,Wang H,Zhang Z

Wed Mar 01 00:00:00 EST 2023

HIF-1α Mediates Arsenic-Induced Metabolic Reprogramming in Lung Bronchial Epithelial Cells.

Biological trace element research

Wang W,Zhang A

Mon May 01 00:00:00 EDT 2023

NR3C2 inhibits the proliferation of colorectal cancer via regulating glucose metabolism and phosphorylating AMPK.

Journal of cellular and molecular medicine

Liu H,Lei W,Li Z,Wang X,Zhou L

Sat Apr 01 00:00:00 EDT 2023

Alamandine/MrgD axis prevents TGF-β1-mediated fibroblast activation via regulation of aerobic glycolysis and mitophagy.

Journal of translational medicine

Wang W,Zhang Y,Huang W,Yuan Y,Hong Q,Xie Z,Li L,Chen Y,Li X,Meng Y

Fri Jan 13 00:00:00 EST 2023

Alamandine/MrgD axis prevents TGF-β1-mediated fibroblast activation via regulation of aerobic glycolysis and mitophagy.

Journal of translational medicine

Wang W,Zhang Y,Huang W,Yuan Y,Hong Q,Xie Z,Li L,Chen Y,Li X,Meng Y

Fri Jan 13 00:00:00 EST 2023

Alamandine/MrgD axis prevents TGF-β1-mediated fibroblast activation via regulation of aerobic glycolysis and mitophagy.

Journal of translational medicine

Wang W,Zhang Y,Huang W,Yuan Y,Hong Q,Xie Z,Li L,Chen Y,Li X,Meng Y

Fri Jan 13 00:00:00 EST 2023

Alamandine/MrgD axis prevents TGF-β1-mediated fibroblast activation via regulation of aerobic glycolysis and mitophagy.

Journal of translational medicine

Wang W,Zhang Y,Huang W,Yuan Y,Hong Q,Xie Z,Li L,Chen Y,Li X,Meng Y

Fri Jan 13 00:00:00 EST 2023

TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties.

Stem cell research & therapy

Cao M,Liu Z,You D,Pan Y,Zhang Q

Thu May 04 00:00:00 EDT 2023

Bleomycin restricts the glycolysis of lymphatic endothelial cells by inhibiting dimeric PKM2 formation: A novel mechanism for lymphatic malformation treatment.

Biochemical pharmacology

Chai M,Wang R,Jiang H,Zhu T,Liu J,Huang C,Zhong W,Cai Y

Sat Oct 01 00:00:00 EDT 2022

Phosphorylation and acetylation of glycolytic enzymes cooperatively regulate their activity and lamb meat quality.

Food chemistry

Ren C,Li X,Bai Y,Schroyen M,Zhang D

Thu Dec 15 00:00:00 EST 2022

Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.

Journal of cellular and molecular medicine

Li L,Li Z,Qu J,Wei X,Suo F,Xu J,Liu X,Chen C,Zheng S

Tue Mar 01 00:00:00 EST 2022

Engineering of thick human functional myocardium via static stretching and electrical stimulation.

iScience

Pretorius D,Kahn-Krell AM,LaBarge WC,Lou X,Zhang J

Fri Mar 18 00:00:00 EDT 2022

Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.

Frontiers in oncology

Wang R,Li J,Zhang C,Guan X,Qin B,Jin R,Qin L,Xu S,Zhang X,Liu R,Ye Q,Cheng L

Sat Jul 16 00:00:00 EDT 2022

Tanshinone IIA inhibits cell growth by suppressing SIX1-induced aerobic glycolysis in non-small cell lung cancer cells.

Oncology letters

Qi H,Chen Z,Qin Y,Wang X,Zhang Z,Li Y

Wed Jun 01 00:00:00 EDT 2022

Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.

Acta pharmacologica Sinica

Xu L,Yang TY,Zhou YW,Wu MF,Shen J,Cheng JL,Liu QX,Cao SY,Wang JQ,Zhang L

Tue Feb 01 00:00:00 EST 2022

The MBNL1/circNTRK2/PAX5 pathway regulates aerobic glycolysis in glioblastoma cells by encoding a novel protein NTRK2-243aa.

Cell death & disease

Zhao Y,Song J,Dong W,Liu X,Yang C,Wang D,Xue Y,Ruan X,Liu L,Wang P,Zhang M,Liu Y

Mon Sep 05 00:00:00 EDT 2022

Myosin 1b promotes migration, invasion and glycolysis in cervical cancer via ERK/HIF-1α pathway.

American journal of translational research

Wen LJ,Hu XL,Li CY,Liu J,Li ZY,Li YZ,Zhou JY

Tue Dec 28 00:00:00 EST 2021

RSL3 enhances the antitumor effect of cisplatin on prostate cancer cells via causing glycolysis dysfunction.

Biochemical pharmacology

Li M,Chen X,Wang X,Wei X,Wang D,Liu X,Xu L,Batu W,Li Y,Guo B,Zhang L

Fri Oct 01 00:00:00 EDT 2021

Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.

Evidence-based complementary and alternative medicine : eCAM

Yang K,Wu K,Feng J,Yutian L,Zhu X,Xu D

Mon Apr 25 00:00:00 EDT 2022

Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease.

Frontiers in pharmacology

Zhou Y,Wu M,Xu L,Cheng J,Shen J,Yang T,Zhang L

Fri Apr 02 00:00:00 EDT 2021

Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection.

Virologica Sinica

Li M,Yang J,Ye C,Bian P,Yang X,Zhang H,Luo C,Xue Z,Lei Y,Lian J

Wed Dec 01 00:00:00 EST 2021

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

International journal of molecular sciences

Li Y,Kang J,Fu J,Luo H,Liu Y,Li Y,Sun L

Wed Mar 03 00:00:00 EST 2021

MiR-489-3p Reduced Pancreatic Cancer Proliferation and Metastasis By Targeting PKM2 and LDHA Involving Glycolysis.

Frontiers in oncology

Zhang D,He Z,Shen Y,Wang J,Liu T,Jiang J

Thu Apr 28 00:00:00 EDT 2022

Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.

Frontiers in oncology

Tao Y,Shao F,Cai M,Liu Z,Peng Y,Huang Q,Meng F

Tue Sep 21 00:00:00 EDT 2021

Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.

Frontiers in oncology

Tao Y,Shao F,Cai M,Liu Z,Peng Y,Huang Q,Meng F

Tue Sep 21 00:00:00 EDT 2021

Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.

Frontiers in oncology

Tao Y,Shao F,Cai M,Liu Z,Peng Y,Huang Q,Meng F

Tue Sep 21 00:00:00 EDT 2021

Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.

Frontiers in oncology

Tao Y,Shao F,Cai M,Liu Z,Peng Y,Huang Q,Meng F

Tue Sep 21 00:00:00 EDT 2021

Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.

Biochemical pharmacology

Deng X,Zhao J,Qu L,Duan Z,Fu R,Zhu C,Fan D

Sat Aug 01 00:00:00 EDT 2020

Ginsenoside Rh4 suppresses aerobic glycolysis and the expression of PD-L1 via targeting AKT in esophageal cancer.

Biochemical pharmacology

Deng X,Zhao J,Qu L,Duan Z,Fu R,Zhu C,Fan D

Sat Aug 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738

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Gene Aliases: AI642394; HK2; HKII; HXK2

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UniProt ID: (Human) P52789, (Mouse) O08528, (Rat) P27881

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Entrez Gene ID: (Human) 3099, (Mouse) 15277, (Rat) 25059

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Function(s)

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity nucleotide binding catalytic activity kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor kinase

Process(es)

cellular glucose homeostasis response to ischemia glycolytic process lactation apoptotic mitochondrial changes glucose transport negative regulation of mitochondrial membrane permeability regulation of glucose import carbohydrate phosphorylation glucose 6-phosphate metabolic process canonical glycolysis establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of mitophagy in response to mitochondrial depolarization negative regulation of reactive oxygen species metabolic process carbohydrate metabolic process glucose metabolic process metabolic process phosphorylation glucose catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HK2 Monoclonal Antibody (2A11C3)

HK2 Antibody (66974-1-IG) in IHC (P)

Product Details

66974-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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