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Product Details
PA5-30600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human HLA-E Recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548074
Product Specific Information
Recommended positive controls: Raji, K562, THP-1, HL-60.
Predicted reactivity: Human (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; lymphocyte antigen; MHC 1; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1
Gene Aliases: EA1.2; EA2.1; HLA-6.2; HLA-E; HLAE; MHC; QA1
UniProt ID: (Human) P13747
Entrez Gene ID: (Human) 3133
positive regulation of T cell mediated cytotoxicity
adaptive immune response
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class Ib
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent
regulation of natural killer cell mediated immunity
positive regulation of natural killer cell mediated immunity
antibacterial humoral response
positive regulation of interleukin-13 production
positive regulation of interleukin-4 production
positive regulation of TRAIL production
positive regulation of tumor necrosis factor production
CD8-positive, alpha-beta T cell activation
protection from natural killer cell mediated cytotoxicity
innate immune response
regulation of immune response
defense response to Gram-positive bacterium
positive regulation of immunoglobulin secretion
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
positive regulation of CD8-positive, alpha-beta T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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