Proteintech

HLA-G Monoclonal Antibody (1E5A10)

HLA-G Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

HLA-G Antibody (66447-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 66447-1-IG ( HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

66447-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:20,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1E5A10

Immunogen

HLA-G Fusion Protein Ag10839 (26-308 aa encoded by BC021708)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SHSMRYFSA AVSRPSRGEP RFIAMGYVDD TQFVRFDSDS ACPRMEPRAP WVEREGPEYW EEETRNTKAH AQTDRMNLQT LRGYYNQSEA SSHTLQWMIG CDLGSDGRLL RGYEQYAYDG KDYLALNEDL RSWTAADTAA QISKRKCEAA NVAEQRRAYL EGTCVEWLHR YLENGKEMLQ RADPPKTHVT HHPVFDYEAT LRCWALGFYP AEIILTWQRD GEDQTQDVEL VETKPAGDGT FQKWAAVVVP SGEEQRYTCH VQHEGLPEPL MLRWKQSSLP TIPI (26-308 aa encoded by BC021708)

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CCL21/CCR7 Axis Contributes to Trophoblastic Cell Migration and Invasion in Preeclampsia by Affecting the Epithelial Mesenchymal Transition via the ERK1/2 Signaling Pathway.

Biology

Liu Z,He J,Jin P,Ran Y,Yin N,Qi H

Wed Jan 18 00:00:00 EST 2023

Profilin1 Regulates Trophoblast Invasion and Macrophage Differentiation.

The American journal of pathology

Chen X,Song QL,Wang JY,Ji R,Li ZH,Cao ML,Mu XF,Guo DY,Zhang Y,Yang J

Tue Aug 01 00:00:00 EDT 2023

The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia.

International journal of molecular sciences

Pang H,Lei D,Chen T,Liu Y,Fan C

Tue Mar 07 00:00:00 EST 2023

The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia.

International journal of molecular sciences

Pang H,Lei D,Chen T,Liu Y,Fan C

Tue Mar 07 00:00:00 EST 2023

ANKRD37 inhibits trophoblast migration and invasion by regulating the NF-κB pathway in preeclampsia.

The journal of gene medicine

Tan W,Fu H,Zhou X,Duan Y,Yin N,Huang J,Liu X

Sun May 01 00:00:00 EDT 2022

The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing.

Frontiers in cell and developmental biology

Ma J,Liu Y,Guo Z,Sun R,Yang X,Zheng W,Ma Y,Rong Y,Wang H,Yang H,Xiao Z

Fri Nov 25 00:00:00 EST 2022

The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing.

Frontiers in cell and developmental biology

Ma J,Liu Y,Guo Z,Sun R,Yang X,Zheng W,Ma Y,Rong Y,Wang H,Yang H,Xiao Z

Fri Nov 25 00:00:00 EST 2022

Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.

Oxidative medicine and cellular longevity

Chen X,Song QL,Li ZH,Ji R,Wang JY,Ge C,Xiao ZN,Guo DY,Yang J

Tue May 24 00:00:00 EDT 2022

Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.

Oxidative medicine and cellular longevity

Chen X,Song QL,Li ZH,Ji R,Wang JY,Ge C,Xiao ZN,Guo DY,Yang J

Tue May 24 00:00:00 EDT 2022

Elabela: Negative Regulation of Ferroptosis in Trophoblasts via the Ferritinophagy Pathway Implicated in the Pathogenesis of Preeclampsia.

Cells

Yang H,Zhang X,Ding Y,Xiong H,Xiang S,Wang Y,Li H,Liu Z,He J,Tao Y,Yang H,Qi H

Mon Dec 26 00:00:00 EST 2022

miR21 modulates the Hippo signaling pathway via interference with PP2A Bβ to inhibit trophoblast invasion and cause preeclampsia.

Molecular therapy. Nucleic acids

Hu M,Zheng Y,Liao J,Wen L,Cheng J,Huang J,Huang B,Lin L,Long Y,Wu Y,Ye X,Fu Y,Qi H,Baker PN,Tong C

Tue Dec 13 00:00:00 EST 2022

MiR-187 regulates the proliferation, migration and invasion of human trophoblast cells by repressing BCL6-mediated activation of PI3K/AKT signaling.

Placenta

Chen X,Song QL,Ji R,Wang JY,Li ZH,Guo D,Yin TL,Wang SJ,Yang J

Tue Feb 01 00:00:00 EST 2022

Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible role in preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Sun M,Gao J,Meng T,Liu S,Chen H,Liu Q,Xing X,Zhao C,Luo Y

Mon Feb 01 00:00:00 EST 2021

Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Reproductive biology and endocrinology : RB&E

Peng W,Liu Y,Qi H,Li Q

Tue Mar 23 00:00:00 EDT 2021

Iodothyronine deiodinase 2 (DiO₂) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage.

Placenta

Adu-Gyamfi EA,Lamptey J,Chen XM,Li FF,Li C,Ruan LL,Yang XN,Liu TH,Wang YX,Ding YB

Sun Aug 01 00:00:00 EDT 2021

Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.

Aging cell

Xiong L,Ye X,Chen Z,Fu H,Li S,Xu P,Yu J,Wen L,Gao R,Fu Y,Qi H,Kilby MD,Saffery R,Baker PN,Tong C

Fri Oct 01 00:00:00 EDT 2021

Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.

Aging cell

Xiong L,Ye X,Chen Z,Fu H,Li S,Xu P,Yu J,Wen L,Gao R,Fu Y,Qi H,Kilby MD,Saffery R,Baker PN,Tong C

Fri Oct 01 00:00:00 EDT 2021

Stomatin-like protein 2 (SLP2) regulates the proliferation and invasion of trophoblast cells by modulating mitochondrial functions.

Placenta

Zhang X,Li BY,Fu LJ,Adu-Gyamfi EA,Xu BR,Liu TH,Chen XM,Lan X,Wang YX,Xu HB,Ding YB

Thu Oct 01 00:00:00 EDT 2020

Stomatin-like protein 2 (SLP2) regulates the proliferation and invasion of trophoblast cells by modulating mitochondrial functions.

Placenta

Zhang X,Li BY,Fu LJ,Adu-Gyamfi EA,Xu BR,Liu TH,Chen XM,Lan X,Wang YX,Xu HB,Ding YB

Thu Oct 01 00:00:00 EDT 2020

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng W,Tong C,Li L,Huang C,Ran Y,Chen X,Bai Y,Liu Y,Zhao J,Tan B,Luo X,Wang H,Wen L,Zhang C,Zhang H,Ding Y,Qi H,Baker PN

Wed May 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G

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Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G

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UniProt ID: (Human) P17693

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Entrez Gene ID: (Human) 3135

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Function(s)

antigen binding receptor binding peptide antigen binding protein homodimerization activity major histocompatibility complex protein

Process(es)

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent positive regulation of tolerance induction positive regulation of T cell tolerance induction immune response-inhibiting cell surface receptor signaling pathway cellular defense response antigen processing and presentation positive regulation of interleukin-12 production negative regulation of T cell proliferation positive regulation of regulatory T cell differentiation regulation of immune response negative regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway negative regulation of dendritic cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLA-G Monoclonal Antibody (1E5A10)

HLA-G Antibody (66447-1-IG) in IHC (P)

Product Details

66447-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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