Invitrogen

HMGB1 Recombinant Rabbit Monoclonal Antibody (HL2059)

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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

MA5-50368

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Dog, Cat, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HL2059

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094379

Product Specific Information

MA5-50368 has successfully been used in Indirect ELISA.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Amphoterin; Amphoterin antibody; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; HMGB1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein

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Gene Aliases: Ac2-008; amphoterin; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1

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UniProt ID: (Human) P09429, (Dog) Q6YKA4, (Rat) P63159, (Mouse) P63158

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Entrez Gene ID: (Human) 3146, (Dog) 403170, (Rat) 25459, (Mouse) 15289

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Function(s)
four-way junction DNA binding bubble DNA binding lipopolysaccharide binding phosphatidylserine binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity protein binding transcription factor binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity poly(A) RNA binding RAGE receptor binding DNA polymerase binding repressing transcription factor binding supercoiled DNA binding transcription coactivator activity integrin binding non-sequence-specific DNA binding, bending open form four-way junction DNA binding crossed form four-way junction DNA binding DNA binding bent DNA binding chromatin binding 5S rRNA binding heparin binding peptide binding protein dimerization activity glycolipid binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter eye development myeloid dendritic cell activation endothelial cell proliferation activation of innate immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response dendritic cell chemotaxis inflammatory response to antigenic stimulus regulation of tolerance induction regulation of T cell mediated immune response to tumor cell DNA topological change base-excision repair apoptotic DNA fragmentation DNA recombination regulation of transcription from RNA polymerase II promoter autophagy inflammatory response positive regulation of cytosolic calcium ion concentration regulation of autophagy negative regulation of RNA polymerase II transcriptional preinitiation complex assembly lung development neuron projection development chromatin assembly regulation of restriction endodeoxyribonuclease activity activation of protein kinase activity DNA geometric change positive regulation of mismatch repair negative regulation of interferon-gamma production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of tumor necrosis factor production V(D)J recombination positive regulation of toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway T-helper 1 cell activation endothelial cell chemotaxis positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of DNA binding positive regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation innate immune response positive regulation of myeloid cell differentiation positive regulation of glycogen catabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of interleukin-1 secretion positive regulation of interleukin-1 beta secretion positive chemotaxis DNA ligation involved in DNA repair positive regulation of DNA ligation response to glucocorticoid positive regulation of ERK1 and ERK2 cascade positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis tumor necrosis factor secretion negative regulation of apoptotic cell clearance positive regulation of interleukin-6 secretion regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation cell morphogenesis positive regulation of protein phosphorylation positive regulation of mesenchymal cell proliferation base-excision repair, DNA ligation chromatin remodeling regulation of transcription, DNA-templated chemotaxis nervous system development circadian rhythm negative regulation of DNA replication positive regulation of cell proliferation response to heat response to glucose positive regulation of cell death positive regulation of neuron projection development positive regulation of smooth muscle cell migration positive regulation of cell migration actin cytoskeleton reorganization response to lipopolysaccharide response to insulin positive regulation of myeloid cell apoptotic process response to interferon-gamma response to drug positive regulation of myoblast differentiation positive regulation of protein kinase activity positive regulation of mitotic cell cycle regulation of inflammatory response male-specific defense response to bacterium induction of positive chemotaxis myoblast proliferation cellular response to interleukin-1 chromatin silencing positive regulation of interleukin-8 production positive regulation of blood vessel endothelial cell migration positive regulation of vascular endothelial cell proliferation positive regulation of chemokine (C-X-C motif) ligand 2 production
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