Invitrogen

HMOX1 Polyclonal Antibody

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Antibody Testing Data (11)
Published Figures (8)

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HMOX1 Antibody (PA5-27338) in ELISA

Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using HMOX1 Monoclonal Antibody (GT17811) (Product # MA5-31558) as capture antibody at concentration of 5 µg/mL and HMOX1 Polyclonal Antibody (Product # PA5-27338) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10,000 and used to detect the primary antibody.

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMOX1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-27338

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544814

Product Specific Information

Recommended positive controls: HeLa, Raw264.7, C2C12, rat spleen, HeLa (500 µM CoCl2 treatment for 24 hr).

Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sex differences in hearing impairment due to diet-induced obesity in CBA/Ca mice.

Biology of sex differences

Kim SJ,Gajbhiye A,Lyu AR,Kim TH,Shin SA,Kwon HC,Park YH,Park MJ

PA5-27338 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to find significant sexual dimorphism in HFD-induced metabolic alterations and obesity-related hearing loss.

Tue Feb 21 00:00:00 EST 2023

Sex differences in hearing impairment due to diet-induced obesity in CBA/Ca mice.

Biology of sex differences

Kim SJ,Gajbhiye A,Lyu AR,Kim TH,Shin SA,Kwon HC,Park YH,Park MJ

Tue Feb 21 00:00:00 EST 2023

Sex differences in hearing impairment due to diet-induced obesity in CBA/Ca mice.

Biology of sex differences

Kim SJ,Gajbhiye A,Lyu AR,Kim TH,Shin SA,Kwon HC,Park YH,Park MJ

Tue Feb 21 00:00:00 EST 2023

Slingshot homolog-1-mediated Nrf2 sequestration tips the balance from neuroprotection to neurodegeneration in Alzheimer's disease.

Proceedings of the National Academy of Sciences of the United States of America

Cazzaro S,Woo JA,Wang X,Liu T,Rego S,Kee TR,Koh Y,Vázquez-Rosa E,Pieper AA,Kang DE

Published figure using HMOX1 polyclonal antibody (Product # PA5-27338) in Western Blot

Tue Jul 25 00:00:00 EDT 2023

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

PA5-27338 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 EDT 2022

Oxidized low-density lipoprotein causes ribosome reduction and inhibition of protein synthesis in retinal pigment epithelial cells.

Biochemistry and biophysics reports

Giorgianni F,Beranova-Giorgianni S

PA5-27338 was used in Western Blotting to characterize alterations in the RPE proteome triggered by exposure to non-cytotoxic levels of oxidized low-density lipoprotein.

Thu Dec 01 00:00:00 EST 2022

Geniposidic Acid from Eucommia ulmoides Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling.

Molecules (Basel, Switzerland)

Cheng S,Jia H,Zhang Y,Zhou J,Chen X,Wu L,Wang J

PA5-27338 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate whether ESF tea can mitigate cellular oxidative stress.

Mon Dec 05 00:00:00 EST 2022

Chronic Hematuria Increases Chronic Kidney Injury and Epithelial-Mesenchymal Transition in 5/6 Nephrectomy Rats.

Frontiers in medicine

Xiao M,Medipally AK,Biederman L,Satoskar AA,Ivanov I,Rovin BH,Brodsky SV

Published figure using HMOX1 polyclonal antibody (Product # PA5-27338) in Immunohistochemistry

Fri Sep 30 00:00:00 EDT 2022

α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice.

Frontiers in aging neuroscience

Zhang YH,Yan XZ,Xu SF,Pang ZQ,Li LB,Yang Y,Fan YG,Wang Z,Yu X,Guo C,Ao Q

PA5-27338 was used in Western Blotting to find that chronic LA administration significantly increased glucose availability by elevating glucose transporter 3 (GLUT3), GLUT4, vascular endothelial growth factor (VEGF) protein and mRNA level, and heme oxygenase-1 (HO-1) protein level in P301S mouse brains.

Mon Sep 28 00:00:00 EDT 2020

α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice.

Frontiers in aging neuroscience

Zhang YH,Yan XZ,Xu SF,Pang ZQ,Li LB,Yang Y,Fan YG,Wang Z,Yu X,Guo C,Ao Q

Mon Sep 28 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27338 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27338 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27338 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27338 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 EDT 2020

Physalis peruviana L. inhibits ovalbumin‑induced airway inflammation by attenuating the activation of NF‑κB and inflammatory molecules.

International journal of molecular medicine

Park HA,Kwon OK,Ryu HW,Min JH,Park MW,Park MH,Paik JH,Choi S,Paryanto I,Yuniato P,Oh SR,Ahn KS,Lee JW

PA5-27338 was used in Western Blot to indicate that PP has considerable potential in the treatment of allergic asthma.

Mon Apr 01 00:00:00 EDT 2019

D‑Pinitol alleviates cyclosporine A‑induced renal tubulointerstitial fibrosis via activating Sirt1 and Nrf2 antioxidant pathways.

International journal of molecular medicine

Koh ES,Kim S,Kim M,Hong YA,Shin SJ,Park CW,Chang YS,Chung S,Kim HS

Published figure using HMOX1 polyclonal antibody (Product # PA5-27338) in Western Blot

Sun Apr 01 00:00:00 EDT 2018

Hemophagocytosis-mediated keratinization in oral carcinoma in situ and squamous cell carcinoma: a possible histopathogenesis of keratin pearls.

Journal of cellular physiology

Al-Eryani K,Cheng J,Abé T,Yamazaki M,Maruyama S,Tsuneki M,Essa A,Babkair H,Saku T

PA5-27338 was used in immunohistochemistry to study oral carcinoma in situ and squamous cell carcinoma keratinization and the role of erythro-hemophagocytosis in the formation of keratin pearls

Tue Oct 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

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Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901

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Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368

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Function(s)

heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding oxidoreductase activity oxygenase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HMOX1 Polyclonal Antibody

HMOX1 Antibody (PA5-27338) in ELISA

Product Details

PA5-27338

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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