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Invitrogen
HP1 alpha Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
HP1 alpha Antibody (PA5-29879) in ICC/IF
HP1 alpha Polyclonal Antibody detects HP1 alpha protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HP1 alpha protein stained by HP1 alpha Polyclonal Antibody (Product # PA5-29879) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-29879
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 138 of Human HP1 alpha
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547353
Product Specific Information
Recommended positive controls: HeLa, HeLa nucleus.
Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (80%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen p25; Cbx5 antibody; chromobox homolog 5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; heterochromatin protein 1 alpha; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; mHP1(alpha)
Gene Aliases: 2610029O15Rik; C75991; CBX5; HEL25; HP1; HP1A; Hp1alpha
UniProt ID: (Human) P45973, (Mouse) Q61686
Entrez Gene ID: (Human) 23468, (Mouse) 12419
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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