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Invitrogen
HP1 alpha Recombinant Mouse Monoclonal Antibody, Biotin
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
HP1 alpha Antibody (730046) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of NTERA-2 (Lane 1), HeLa (lane 2), HEK-293 (Lane 3), K-562 (Lane 4) and A549 (Lane 5). The blots were probed with Anti- CBX-5 Recombinant Mouse monoclonal Antibody (Product # 730046, 2 µg/mL) and detected by chemiluminescence Biotin conjugated streptavidin Poly HRP (Product # 21140, 1:10000 dilution). A 22kDa band corresponding to CBX-5 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris ge... View More
Product Details
730046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Immunogen
18-75 of 191 of CBX5 Protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2532847
Product Specific Information
730046 is a biotinylated Fab fragment targeting HP1 alpha.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Antigen p25; Cbx5 antibody; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha
Gene Aliases: CBX5; HEL25; HP1; HP1A
UniProt ID: (Human) P45973
Entrez Gene ID: (Human) 23468
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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