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HRH1 Antibody (PA5-102098) in ICC/IF
Immunocytochemistry analysis of HRH1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HRH1 polyclonal antibody (Product # PA5-102098) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102098
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HRH1(Accession P35367), corresponding to amino acid residues A146-I196.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851525
Product Specific Information
Antibody detects endogenous levels of total HRH1.
Target Information
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H1 receptor; H1-R; Histamine H1 receptor; Histamine H1-R; histamine receptor; histamine receptor, subclass H1
Gene Aliases: H1-R; H1R; HH1R; hisH1; HRH1
UniProt ID: (Human) P35367
Entrez Gene ID: (Human) 3269
inflammatory response
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
memory
visual learning
regulation of vasoconstriction
positive regulation of inositol trisphosphate biosynthetic process
regulation of vascular permeability
positive regulation of vasoconstriction
inositol phosphate-mediated signaling
regulation of synaptic plasticity
eosinophil chemotaxis
modulation of synaptic transmission
cellular response to histamine
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