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FIGURE: 1 / 1
HRI Antibody (PA5-86921) in WB
Western blot analysis of eIF2 beta in Lane 1: K562 whole cell lysate (40 µg), Lane 2: L02 whole cell lysate (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: AML-12whole cell lysate (40 µg). Sample was incubated with eIF2 beta polyclonal antibody (Product # PA5-86921) using a 1:500 dilution.
Product Details
PA5-86921
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 37-586 of Human EIF2AK1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2803679
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
EIF2AK1 (HRI) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK1, signaling is initiated by low levels of heme. The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands and kinase activity is induced by low levels of heme and inhibited by the presence of heme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; he; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; hemin-sensitive initiation factor 2a kinase; hHRI
Gene Aliases: EIF2AK1; HCR; HRI; KIAA1369; PRO1362
UniProt ID: (Human) Q9BQI3, (Rat) Q63185, (Mouse) Q9CTP5
Entrez Gene ID: (Human) 27102, (Rat) 27137, (Mouse) 15467
eukaryotic translation initiation factor 2alpha kinase activity
ATP binding
heme binding
protein homodimerization activity
protein kinase activity
nucleotide binding
protein serine/threonine kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
acute inflammatory response
phagocytosis
response to stress
negative regulation of cell proliferation
response to external stimulus
regulation of translational initiation by eIF2 alpha phosphorylation
regulation of eIF2 alpha phosphorylation by heme
macrophage differentiation
negative regulation of translational initiation by iron
protoporphyrinogen IX metabolic process
protein autophosphorylation
negative regulation of hemoglobin biosynthetic process
iron ion homeostasis
translational initiation
regulation of translation
protein phosphorylation
negative regulation of translational initiation
regulation of hemoglobin biosynthetic process
phosphorylation
negative regulation of translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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