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Product Details
PA5-20235
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 15 amino acid peptide from near the center of human Hrk.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153786
Product Specific Information
Despite its predicted molecular weight, Hrk often migrates at 12-15kDa in SDS-PAGE. A suggested positive control is mouse pancreas tissue lysate.
PA5-20235 can be used with blocking peptide PEP-0369.
Target Information
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator of apoptosis harakiri; activator of apoptosis Hrk; BCL2-interacting protein; BH3 interacting (with BCL2 family) domain, apoptosis agonist; BH3-interacting domain-containing protein 3; death protein 5; harakiri, BCL2 interacting protein (contains only BH3 domain); Neuronal death protein DP5
Gene Aliases: AI838259; BID3; DP5; HARAKIRI; HRK
UniProt ID: (Human) O00198, (Mouse) P62816
Entrez Gene ID: (Human) 8739, (Mouse) 12123
apoptotic process
positive regulation of protein homooligomerization
positive regulation of apoptotic process
positive regulation of release of cytochrome c from mitochondria
regulation of apoptotic process
positive regulation of neuron apoptotic process
cellular response to potassium ion starvation
Performance Guarantee
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