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HSC70 Antibody (PA5-116380) in WB
Western Blot analysis of HSC70 using the HSC70 Polyclonal Antibody (Product # PA5-116380) at a 1:2000 dilution. Lane 1: Zebrafish lysate, Lane 2: Zebrafish muscle lysate, Lane 3: ZF4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-116380
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Zebrafish
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 528-562 amino acids from the C-terminal region of DANRE hspa8
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901014
Target Information
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AAG2; FAM10A1; heat shock 70 kDa protein 8; heat shock 70kDa protein 8; heat shock cognate 71-kd protein; MGC131511; MGC29929; SNC6
Gene Aliases: hsc70; wu:fb01g06; wu:fi48b06
Entrez Gene ID: (Zebrafish) 573376
response to unfolded protein
vesicle-mediated transport
fin regeneration
cellular response to unfolded protein
regulation of fibroblast growth factor receptor signaling pathway
protein refolding
positive regulation of receptor-mediated endocytosis
chaperone mediated protein folding requiring cofactor
protein localization to early endosome
Performance Guarantee
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