Invitrogen

HSC70 Monoclonal Antibody (13D3)

9 Published Figures

27 References

View all (36) HSC70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)
Published Figures (9)

HSC70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

HSC70 Antibody (MA3-014) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 70 (HSC70) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSC70 monoclonal antibody (Product # MA3-014) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:40... View More

HSC70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSC70 Antibody in Immunoprecipitation (IP)

HSC70 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

HSC70 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

HSC70 Antibody in Immunohistochemistry (IHC)

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Product Details

MA3-014

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µL

Neutralization (Neu)

View 1 publication 1 publication

Competition Assay (CA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Cat, Human, Mouse, Non-human primate, Rat

Published species

Bovine, Cat, Fish, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

13D3

Immunogen

Mouse spermatogenic cell protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325462

Product Specific Information

MA3-014 detects heat shock cognate protein 70 kDa (HSC70) from human, feline, primate, rat, and mouse tissues. This antibody displays slight cross-reactivity to HSP70.

MA3-014 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation procedures By Western blot, this antibody detects a 70 kDa protein representing HSC70. By 2D gel electrophoresis, MA3-014 was found to bind strongly to HSC70 and faintly to HSP70 both before and after heat shock. Immunohistochemical staining of HSC70 in mouse spermatids/spermatozoa with MA3-014 results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa.

The MA3-014 antigen is mouse spermatogenic cell protein.

Target Information

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells.

Frontiers in cell and developmental biology

Reyes Ballista JM,Miazgowicz KL,Acciani MD,Jimenez AR,Belloli RS,Havranek KE,Brindley MA

MA3-014 was used in Competition Assay to provide data which denotes the importance of careful evaluation of reagents used to identify receptor use in invertebrate cells.

Fri May 19 00:00:00 EDT 2023

Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells.

Frontiers in cell and developmental biology

Reyes Ballista JM,Miazgowicz KL,Acciani MD,Jimenez AR,Belloli RS,Havranek KE,Brindley MA

MA3-014 was used in Competition Assay to provide data which denotes the importance of careful evaluation of reagents used to identify receptor use in invertebrate cells.

Fri May 19 00:00:00 EDT 2023

Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells.

Frontiers in cell and developmental biology

Reyes Ballista JM,Miazgowicz KL,Acciani MD,Jimenez AR,Belloli RS,Havranek KE,Brindley MA

MA3-014 was used in Competition Assay to provide data which denotes the importance of careful evaluation of reagents used to identify receptor use in invertebrate cells.

Fri May 19 00:00:00 EDT 2023

Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues.

Methods in molecular biology (Clifton, N.J.)

Losmanová T,Tschan MP,Galván JA,Berezowska S

MA3-014 was used in Immunohistochemistry-immunofluorescence to detect key players of CMS, HSPA8 and LAMP2A, and present an additional staining evaluation method.

Wed Sep 28 00:00:00 EDT 2022

Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy.

Cells

Losmanova T,Zens P,Scherz A,Schmid RA,Tschan MP,Berezowska S

MA3-014 was used in Immunohistochemistry to investigate chaperone-mediated autophagy (CMA) in non-small cell lung cancer patients after neoadjuvant therapy and primary resection, finding no correlation between CMA markers and neoadjuvant treatment, pathological parameters, or tumor response, except for LAMP2A.

Wed Oct 13 00:00:00 EDT 2021

Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy.

Cells

Losmanova T,Zens P,Scherz A,Schmid RA,Tschan MP,Berezowska S

Wed Oct 13 00:00:00 EDT 2021

Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network.

Molecular cell

Vonk WIM,Rainbolt TK,Dolan PT,Webb AE,Brunet A,Frydman J

MA3-014 was used in Western Blot to find that the NSPC chaperone network robustly maintains misfolded protein solubility and stress resilience through high levels of the ATP-dependent chaperonin TRiC/CCT.

Thu Apr 16 00:00:00 EDT 2020

Chaperone-Mediated Autophagy Markers LAMP2A and HSC70 Are Independent Adverse Prognostic Markers in Primary Resected Squamous Cell Carcinomas of the Lung.

Oxidative medicine and cellular longevity

Losmanová T,Janser FA,Humbert M,Tokarchuk I,Schläfli AM,Neppl C,Schmid RA,Tschan MP,Langer R,Berezowska S

MA3-014 was used in Western Blotting to evaluate the impact of LAMP2A and HSC70 in pulmonary squamous cell carcinomas (pSQCC).

Wed May 12 00:00:00 EDT 2021

Chaperone-Mediated Autophagy Markers LAMP2A and HSC70 Are Independent Adverse Prognostic Markers in Primary Resected Squamous Cell Carcinomas of the Lung.

Oxidative medicine and cellular longevity

Losmanová T,Janser FA,Humbert M,Tokarchuk I,Schläfli AM,Neppl C,Schmid RA,Tschan MP,Langer R,Berezowska S

MA3-014 was used in Western Blotting to evaluate the impact of LAMP2A and HSC70 in pulmonary squamous cell carcinomas (pSQCC).

Wed May 12 00:00:00 EDT 2021

Zika Virus Dependence on Host Hsp70 Provides a Protective Strategy against Infection and Disease.

Cell reports

Taguwa S,Yeh MT,Rainbolt TK,Nayak A,Shao H,Gestwicki JE,Andino R,Frydman J

Published figure using HSC70 monoclonal antibody (Product # MA3-014) in Immunocytochemistry

Tue Jan 22 00:00:00 EST 2019

Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons.

Neuron

Cao M,Wu Y,Ashrafi G,McCartney AJ,Wheeler H,Bushong EA,Boassa D,Ellisman MH,Ryan TA,De Camilli P

MA3014 was used in western blot to elucidate the contribution of the Sac phosphatase domain of Synaptojanin 1 to neurological symptoms

Wed Feb 22 00:00:00 EST 2017

Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line.

Intervirology

Ghosh A,Desai A,Ravi V,Narayanappa G,Tyagi BK

Published figure using HSC70 monoclonal antibody (Product # MA3-014) in Immunohistochemistry

Wed Nov 20 00:00:00 EST 2019

Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line.

Intervirology

Ghosh A,Desai A,Ravi V,Narayanappa G,Tyagi BK

Published figure using HSC70 monoclonal antibody (Product # MA3-014) in Immunohistochemistry

Wed Nov 20 00:00:00 EST 2019

Chikungunya Virus Interacts with Heat Shock Cognate 70 Protein to Facilitate Its Entry into Mosquito Cell Line.

Intervirology

Ghosh A,Desai A,Ravi V,Narayanappa G,Tyagi BK

Published figure using HSC70 monoclonal antibody (Product # MA3-014) in Immunohistochemistry

Wed Nov 20 00:00:00 EST 2019

Two new isoforms of the human hepatoma-derived growth factor interact with components of the cytoskeleton.

Biological chemistry

Nüße J,Mirastschijski U,Waespy M,Oetjen J,Brandes N,Rebello O,Paroni F,Kelm S,Dietz F

MA3-014 was used in western blot to study the interaction of components of the cytoskeleton with two new isoforms of the human hepatoma-derived growth factor

Sun May 01 00:00:00 EDT 2016

Proteomic analysis of exosomes derived from human lymphoma cells.

European journal of medical research

Yao Y,Wei W,Sun J,Chen L,Deng X,Ma L,Hao S

MA3-014 was used in western blot to characterize the proteomic content of lymphoma cell-derived exosomes.

Thu Jan 29 00:00:00 EST 2015

Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions.

Archives of biochemistry and biophysics

Reddy VS,Kumar ChU,Raghu G,Reddy GB

MA3-014 was used in western blot to examine the expression of sHsp under chronic hyperglycemic conditions in rat heart

Mon Sep 15 00:00:00 EDT 2014

Response of small heat shock proteins in diabetic rat retina.

Investigative ophthalmology & visual science

Reddy VS,Raghu G,Reddy SS,Pasupulati AK,Suryanarayana P,Reddy GB

MA3-014 was used in immunohistochemistry and western blot to study the expression of small heat shock proteins in the retina of diabetic rats

Tue Nov 19 00:00:00 EST 2013

Dissecting the role of integrin subunits alpha 2 and beta 3 in rotavirus cell entry by RNA silencing.

Virus research

Isa P,Sánchez-Alemán MA,López S,Arias CF

MA3-014 was used in flow cytometry to study the role of integrin subunits alpha2 and beta3 in rotavirus cell entry using RNA silencing

Sun Nov 01 00:00:00 EDT 2009

Proteomic analysis of exosomes secreted by human mesothelioma cells.

The American journal of pathology

Hegmans JP,Bard MP,Hemmes A,Luider TM,Kleijmeer MJ,Prins JB,Zitvogel L,Burgers SA,Hoogsteden HC,Lambrecht BN

MA3-014 was used in western blot to conduct the proteomic profiling of exosomes secreted by human mesothelioma cells.

Sat May 01 00:00:00 EDT 2004

Heat shock cognate protein 70 is involved in rotavirus cell entry.

Journal of virology

Guerrero CA,Bouyssounade D,Zárate S,Isa P,López T,Espinosa R,Romero P,Méndez E,López S,Arias CF

MA3-014 was used in blocking/activating experiment, flow cytometry and immunocytochemistry to investigate the role of hsc70 in rotavirus cell entry

Mon Apr 01 00:00:00 EST 2002

Heat shock cognate protein 70 is involved in rotavirus cell entry.

Journal of virology

Guerrero CA,Bouyssounade D,Zárate S,Isa P,López T,Espinosa R,Romero P,Méndez E,López S,Arias CF

MA3-014 was used in blocking/activating experiment, flow cytometry and immunocytochemistry to investigate the role of hsc70 in rotavirus cell entry

Mon Apr 01 00:00:00 EST 2002

Heat shock cognate protein 70 is involved in rotavirus cell entry.

Journal of virology

Guerrero CA,Bouyssounade D,Zárate S,Isa P,López T,Espinosa R,Romero P,Méndez E,López S,Arias CF

MA3-014 was used in blocking/activating experiment, flow cytometry and immunocytochemistry to investigate the role of hsc70 in rotavirus cell entry

Mon Apr 01 00:00:00 EST 2002

The repeated bout effect and heat shock proteins: intramuscular HSP27 and HSP70 expression following two bouts of eccentric exercise in humans.

Acta physiologica Scandinavica

Thompson HS,Clarkson PM,Scordilis SP

MA3-014 was used in western blot to investigate the changes of HSP27 and HSP70 expression induced by eccentric exercise in humans

Tue Jan 01 00:00:00 EST 2002

Characterization and regulation of the major histocompatibility complex-encoded proteins Hsp70-Hom and Hsp70-1/2.

Cell stress & chaperones

Fourie AM,Peterson PA,Yang Y

Published figure using HSC70 monoclonal antibody (Product # MA3-014) in Western Blot

Sun Jul 01 00:00:00 EDT 2001

Characterization and regulation of the major histocompatibility complex-encoded proteins Hsp70-Hom and Hsp70-1/2.

Cell stress & chaperones

Fourie AM,Peterson PA,Yang Y

Published figure using HSC70 monoclonal antibody (Product # MA3-014) in Western Blot

Sun Jul 01 00:00:00 EDT 2001

Heat shock cognate-70 gene expression declines during normal aging of the primate retina.

Investigative ophthalmology & visual science

Bernstein SL,Liu AM,Hansen BC,Somiari RI

MA3-014 was used in western blot to characterize gene expression in the primate retinas during aging.

Fri Sep 01 00:00:00 EDT 2000

Blocking the endogenous increase in HSP 72 increases susceptibility to hypoxia and reoxygenation in isolated adult feline cardiocytes.

Circulation

Nakano M,Mann DL,Knowlton AA

MA3-014 was used in western blot to investigate the consequences of inhibiting the upregulation of endogenous HSP 72 in cardiac myocytes in response to hypoxic stress

Tue Mar 18 00:00:00 EST 1997

A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family.

The Journal of biological chemistry

Leung SM,Hightower LE

MA3-014 was used in western blot to characterize a new Nm23/nucleoside diphosphate kinase family member

Fri Jan 31 00:00:00 EST 1997

Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells.

Biochemical and biophysical research communications

Nunes SL,Calderwood SK

MA3-014 was used in western blot to investigate the association between HSF-1 and HSC 70 protein in the cytoplasm of NIH-3T3 cells.

Fri Aug 04 00:00:00 EDT 1995

Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein.

The Journal of biological chemistry

Terlecky SR,Chiang HL,Olson TS,Dice JF

MA3-014 was used in western blot to investigate the functional properties of hsc73

Tue May 05 00:00:00 EDT 1992

A novel hsp70-like protein (P70) is present in mouse spermatogenic cells.

Molecular and cellular biology

Allen RL,O'Brien DA,Eddy EM

MA3-014 was used in western blot to investigate the distribution of hsp70-like protein in mouse germ cells

Mon Feb 01 00:00:00 EST 1988

Bioinformatics

Protein Aliases: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; Heat shock protein family A member 8; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; SNC6

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Gene Aliases: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP71; HSP73; HSPA10; HSPA8; LAP-1; LAP1; NIP71

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UniProt ID: (Human) P11142, (Rat) P63018, (Mouse) P63017

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Entrez Gene ID: (Human) 3312, (Rat) 24468, (Mouse) 15481

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Function(s)

G-protein coupled receptor binding phosphatidylserine binding protein binding ATP binding ATPase activity enzyme binding MHC class II protein complex binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled poly(A) RNA binding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion receptor binding ADP binding nucleotide binding

Process(es)

transcription, DNA-templated mRNA processing protein folding RNA splicing protein refolding negative regulation of transcription, DNA-templated positive regulation of mRNA splicing, via spliceosome chaperone mediated protein folding requiring cofactor regulation of cell cycle chaperone-mediated protein folding clathrin coat disassembly negative regulation of fibril organization mRNA splicing, via spliceosome protein targeting to lysosome response to unfolded protein neurotransmitter secretion cellular response to starvation viral process regulation of protein stability regulation of protein complex assembly regulation of mRNA stability ATP metabolic process regulation of protein complex stability chaperone-mediated autophagy late endosomal microautophagy protein targeting to lysosome involved in chaperone-mediated autophagy chaperone-mediated protein transport involved in chaperone-mediated autophagy cell-cell adhesion regulation of cellular response to heat regulation of protein import chaperone-mediated autophagy translocation complex disassembly regulation of transcription, DNA-templated

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HSC70 Monoclonal Antibody (13D3)

HSC70 Antibody (MA3-014) in ICC/IF

Product Details

MA3-014

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Competition Assay (CA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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