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Invitrogen
HSP27 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 13
HSP27 Antibody (PA1-017) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 27 (HSP27) (green) in HeLa cells and negative control NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody (Product # PA1-017), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product # 35552... View More
Product Details
PA1-017
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2120942
Product Specific Information
PA1-017 detects heat shock protein 27(hsp27) from rat, mouse, monkey, and human tissue tissues.
PA1-017 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects a ~27 kDa protein which corresponds to hsp27 from HeLa cells. Immunocytochemical staining of hsp27 in HeLa cells with this antibody results in diffuse cytoplasmic staining before heat shock and diffuse cytoplasmic along with punctate staining patterns after.
The PA1-017 immunogen is a synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27. This peptide is 81% conserved in mice, rats, and hamsters. The phosphorylated peptide (Cat. # PEP-187) and non-phosphorylated peptide (Cat. # PEP-240) are available for use in neutralization and control experiments.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27
Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27
UniProt ID: (Human) P04792
Entrez Gene ID: (Human) 3315, (Rat) 24471
retina homeostasis
regulation of translational initiation
negative regulation of protein kinase activity
movement of cell or subcellular component
response to unfolded protein
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mRNA stability
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein serine/threonine kinase activity
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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