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Invitrogen
HSP27 Recombinant Rabbit Monoclonal Antibody (10C11)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
HSP27 Antibody (MA5-33170) in ICC/IF
Immunofluorescence analysis of HSP27 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HSP27 Recombinant Rabbit Monoclonal Antibody (Product # MA5-33170) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 48... View More
Product Details
MA5-33170
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10C11
Immunogen
A synthesized peptide derived from human HSPB1
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2811986
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27
Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27
UniProt ID: (Human) P04792
Entrez Gene ID: (Human) 3315
retina homeostasis
regulation of translational initiation
negative regulation of protein kinase activity
movement of cell or subcellular component
response to unfolded protein
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mRNA stability
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein serine/threonine kinase activity
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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