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HSP27 Antibody (MA5-45121) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Hsp27 monoclonal antibody (Product # MA5-45121) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Product Details
MA5-45121
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Sheep,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8A7
Immunogen
HSP27 peptide
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931575
Product Specific Information
A 1:5000 dilution of MA5-45121 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 25kDa or approximately 27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 27 kDa heat shock protein 1; 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27 kDa protein 1; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 27 kDa beta-1; Heat shock protein beta-1; Heat shock protein family B member 1; heat shock protein family B member 1 variant 1; heat shock protein family B member 1 variant 2; heat shock protein, 25 kDa; HS; HSP; HSP 25; HSP 27; HspB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25
Gene Aliases: 27kDa; CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; I79_007264; SRP27
UniProt ID: (Human) P04792, (Mouse) P14602
regulation of protein phosphorylation
chaperone-mediated protein folding
anterograde axonal protein transport
negative regulation of protein kinase activity
response to unfolded protein
positive regulation of interleukin-1 beta production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
platelet aggregation
negative regulation of protein serine/threonine kinase activity
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
negative regulation of apoptotic signaling pathway
retina homeostasis
regulation of translational initiation
movement of cell or subcellular component
response to virus
regulation of autophagy
negative regulation of apoptotic process
regulation of mRNA stability
vascular endothelial growth factor receptor signaling pathway
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