Invitrogen

HSP27 Monoclonal Antibody (G3.1)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1 Published Figure

32 References

View all (98) HSP27 antibodies

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Protocols

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

HSP27 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

HSP27 Antibody (MA3-015) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 27 (Hsp27, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp27 monoclonal antibody (Product # MA3-015), at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # ... View More

HSP27 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP27 Antibody in Immunoprecipitation (IP)

HSP27 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

HSP27 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of HSP27 was observed in MCF-7, MDA-MB-231, PC-3, DU 145, HeLa and LNCaP in comparison to HT-1080 using Anti-HSP27 Monoclonal Antibody (G3.1) (Product # MA3-015) in Western Blot. {RE}

Product Details

MA3-015

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

1:500

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µL

View 3 publications 3 publications

Immunomicroscopy (IM)

Assay-dependent

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

G3.1

Immunogen

Partially purified human HSP27.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325463

Product Specific Information

MA3-015 detects heat shock protein 27 kDa (HSP27) from human, rat, mouse, monkey, and canine samples.

MA3-015 has been successfully used in Western blot, immunohistochemistry (paraffin), immunoprecipitation, immunofluorescence, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects an ~24 kDa protein representing HSP27 in MCF-7 cell extract. Immunohistochemical staining of human cervix with MA3-015 results in intense cytoplasmic staining of the epithelium within the parabasal cell layer. Western blot detection of HSP27 in canine heart samples is weak and may require optimization.

The MA3-015 antigen is partially purified HSP27 derived from MCF-7 cytosol. The mouse HSP27 sequence is strongly conserved within the human sequence, so cross-reactivity with mouse HSP27 is expected.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cyanidin 3-O-arabinoside suppresses DHT-induced dermal papilla cell senescence by modulating p38-dependent ER-mitochondria contacts.

Journal of biomedical science

Jung YH,Chae CW,Choi GE,Shin HC,Lim JR,Chang HS,Park J,Cho JH,Park MR,Lee HJ,Han HJ

MA3-015 was used in Western Blot to investigate mechanisms of mitochondrial dysfunction in androgenetic alopecia.

Mon Mar 07 00:00:00 EST 2022

The TLK1-MK5 Axis Regulates Motility, Invasion, and Metastasis of Prostate Cancer Cells.

Cancers

Khalil MI,De Benedetti A

MA3-015 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Western Blot to support that the TLK1-MK5 axis is functionally involved in driving PCa cell metastasis and clinical aggressiveness; hence, disruption of this axis may inhibit the metastatic capacity of PCa.

Tue Nov 22 00:00:00 EST 2022

Constitutively active androgen receptor supports the metastatic phenotype of endocrine-resistant hormone receptor-positive breast cancer.

Cell communication and signaling : CCS

Bahnassy S,Thangavel H,Quttina M,Khan AF,Dhanyalayam D,Ritho J,Karami S,Ren J,Bawa-Khalfe T

MA3-015 was used in Western Blotting to study the accumulation of the androgen receptor in endocrine resistance HR+ BCa cells.

Fri Sep 18 00:00:00 EDT 2020

Constitutively active androgen receptor supports the metastatic phenotype of endocrine-resistant hormone receptor-positive breast cancer.

Cell communication and signaling : CCS

Bahnassy S,Thangavel H,Quttina M,Khan AF,Dhanyalayam D,Ritho J,Karami S,Ren J,Bawa-Khalfe T

MA3-015 was used in Western Blotting to study the accumulation of the androgen receptor in endocrine resistance HR+ BCa cells.

Fri Sep 18 00:00:00 EDT 2020

Constitutively active androgen receptor supports the metastatic phenotype of endocrine-resistant hormone receptor-positive breast cancer.

Cell communication and signaling : CCS

Bahnassy S,Thangavel H,Quttina M,Khan AF,Dhanyalayam D,Ritho J,Karami S,Ren J,Bawa-Khalfe T

MA3-015 was used in Western Blotting to study the accumulation of the androgen receptor in endocrine resistance HR+ BCa cells.

Fri Sep 18 00:00:00 EDT 2020

Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA.

Cell death & disease

Hao Q,Wang P,Dutta P,Chung S,Li Q,Wang K,Li J,Cao W,Deng W,Geng Q,Schrode K,Shaheen M,Wu K,Zhu D,Chen QH,Chen G,Elshimali Y,Vadgama J,Wu Y

MA3-015 was used in Western Blotting to conclude that NUDT3-AS4 may play a proproliferative role in TNBC and be considered a relevant therapeutic target, and Comp34 presents promising activity as a single agent to inhibit TNBC through regulation of NUDT3-AS4 and miR-99s.

Fri Dec 11 00:00:00 EST 2020

Increased FXYD1 and PGC-1α mRNA after blood flow-restricted running is related to fibre type-specific AMPK signalling and oxidative stress in human muscle.

Acta physiologica (Oxford, England)

Christiansen D,Murphy RM,Bangsbo J,Stathis CG,Bishop DJ

MA3-015 was used in Western Blotting to study the effects of blood flow restriction on FXYD1 and Na+ ,K+ -ATPase isoforms mRNA responses to an interval running exercise.

Fri Jun 01 00:00:00 EDT 2018

Small heat shock proteins translocate to the cytoskeleton in human skeletal muscle following eccentric exercise independently of phosphorylation.

Journal of applied physiology (Bethesda, Md. : 1985)

Frankenberg NT,Lamb GD,Overgaard K,Murphy RM,Vissing K

MA3-015 was used in western blot to determine if the type of contraction affects the localization or phosphorylation of sHSPs, HSP27, and alphaB-crystallin in human muscle

Sun Jun 01 00:00:00 EDT 2014

Small-molecule proteostasis regulators for protein conformational diseases.

Nature chemical biology

Calamini B,Silva MC,Madoux F,Hutt DM,Khanna S,Chalfant MA,Saldanha SA,Hodder P,Tait BD,Garza D,Balch WE,Morimoto RI

MA3-015 was used in western blot to identify small-molecule proteostasis regulators and evaluate them

Sun Dec 25 00:00:00 EST 2011

Altered expression of HSP27 and HSP70 in distal oesophageal mucosa in patients with gastro-oesophageal reflux disease subjected to fundoplication.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology

Rantanen T,Honkanen T,Paavonen T,Rantanen L,Oksala N

MA3-015 was used in immunohistochemistry to investigate the effect of heat shock protein 27 and heat shock protein 70 on mucosal defence system of the distal oesophagus

Tue Feb 01 00:00:00 EST 2011

Spatiotemporal expression of Hsp20 and its phosphorylation in hippocampal CA1 pyramidal neurons after transient forebrain ischemia.

Neurological research

Niwa M,Hara A,Taguchi A,Aoki H,Kozawa O,Mori H

MA3-015 was used in immunohistochemistry to study the effect of transient forebrain ischemia on the expression and Phosphorylation of Hsp20 in hippocampal CA1 pyramidal neurons

Tue Sep 01 00:00:00 EDT 2009

[The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis].

Acta orthopaedica et traumatologica turcica

Ozger H,Eralp L,Atalar AC,Toker B,Esberk Ateş L,Sungur M,Bilgiç B,Ayan I

MA3-015 was used in immunohistochemistry to examine the prognostic significance of multiple genes in osteosarcoma

Tue May 26 00:00:00 EDT 2009

Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cells.

Breast cancer research : BCR

Marty B,Maire V,Gravier E,Rigaill G,Vincent-Salomon A,Kappler M,Lebigot I,Djelti F,Tourdès A,Gestraud P,Hupé P,Barillot E,Cruzalegui F,Tucker GC,Stern MH,Thiery JP,Hickman JA,Dubois T

MA3-015 was used in immunohistochemistry to study the genomic changes and pathway deregulation in basal-like breast cancer cells

Tue Apr 07 00:00:00 EDT 2009

fMLP induces Hsp27 expression, attenuates NF-kappaB activation, and confers intestinal epithelial cell protection.

American journal of physiology. Gastrointestinal and liver physiology

Carlson RM,Vavricka SR,Eloranta JJ,Musch MW,Arvans DL,Kles KA,Walsh-Reitz MM,Kullak-Ublick GA,Chang EB

MA3-015 was used in western blot to study how the bacterial chemotactic peptide fMLP stimulates colonic epithelial Hsp expression under physiological conditions

Sun Apr 01 00:00:00 EDT 2007

Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.

Cancer research

Liu Y,Liu H,Han B,Zhang JT

MA3-015 was used in western blot to study the role of 14-3-3sigma in drug resistance in human breast cancer cells

Wed Mar 15 00:00:00 EST 2006

Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells.

Journal of cellular physiology

Caraglia M,Marra M,Mancinelli F,D'Ambrosio G,Massa R,Giordano A,Budillon A,Abbruzzese A,Bismuto E

MA3-015 was used in western blot to study the induction of apoptosis and inactivation of the multi-chaperone complex in human epidermal cancer cells by electromagnetic fields at mobile phone frequency

Mon Aug 01 00:00:00 EDT 2005

Expressions of HSP70 and HSP27 in hepatocellular carcinoma.

Journal of Korean medical science

Joo M,Chi JG,Lee H

MA3-015 was used in immunohistochemistry to study the expressions of heat shock proteins in hepatocellular carcinoma and their role in carcinogenesis

Sat Oct 01 00:00:00 EDT 2005

The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.

The Journal of steroid biochemistry and molecular biology

Sommer S,Cui Y,Brewer G,Fuqua SA

MA3-015 was used in western blot to study the role of the c-Yes 3'-UTR in mRNA decay in breast cancer cells

Tue Nov 01 00:00:00 EST 2005

Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Elpek GO,Karaveli S,Simşek T,Keles N,Aksoy NH

MA3-015 was used in immunohistochemistry to study the expression of heat-shock proteins in malignant epithelial ovarian tumors

Tue Apr 01 00:00:00 EST 2003

Immunohistochemical study of heat shock proteins 27, 60 and 70 in the normal human adrenal and in adrenal tumors with suppressed ACTH production.

Microscopy research and technique

Pignatelli D,Ferreira J,Soares P,Costa MJ,Magalhães MC

MA3-015 was used in immunohistochemistry to investigate the expression of heat shock proteins in normal and tumoral adrenal glands

Sun Jun 15 00:00:00 EDT 2003

Differential proteome analysis of replicative senescence in rat embryo fibroblasts.

Molecular & cellular proteomics : MCP

Benvenuti S,Cramer R,Quinn CC,Bruce J,Zvelebil M,Corless S,Bond J,Yang A,Hockfield S,Burlingame AL,Waterfield MD,Jat PS

MA3-015 was used in western blot to perform a deifferential proteomic analysis of replicative senescence in rat embryo fibroblasts

Mon Apr 01 00:00:00 EST 2002

Polyglutamine expansions cause decreased CRE-mediated transcription and early gene expression changes prior to cell death in an inducible cell model of Huntington's disease.

Human molecular genetics

Wyttenbach A,Swartz J,Kita H,Thykjaer T,Carmichael J,Bradley J,Brown R,Maxwell M,Schapira A,Orntoft TF,Kato K,Rubinsztein DC

MA3-015 was used in immunohistochemistry to investigate the effect of polyglutamine expansions on gene expression changes in a Huntington disease model

Wed Aug 15 00:00:00 EDT 2001

Regulation of human CLC-3 channels by multifunctional Ca2+/calmodulin-dependent protein kinase.

The Journal of biological chemistry

Huang P,Liu J,Di A,Robinson NC,Musch MW,Kaetzel MA,Nelson DJ

MA3-015 was used in immunocytochemistry to study the regulatory mechanism for CLC-3 channels by calcium/calmodulin-dependent protein kinase in human.

Fri Jun 08 00:00:00 EDT 2001

The Fas-induced apoptosis analyzed by high throughput proteome analysis.

The Journal of biological chemistry

Gerner C,Frohwein U,Gotzmann J,Bayer E,Gelbmann D,Bursch W,Schulte-Hermann R

MA3-015 was used in western blot to perform a proteomic analysis of Jurkat T-cell cytosolic proteins during Fas-induced cell death

Fri Dec 15 00:00:00 EST 2000

Differential expression of Hsp27 in normal oesophagus, Barrett's metaplasia and oesophageal adenocarcinomas.

British journal of cancer

Soldes OS,Kuick RD,Thompson IA,Hughes SJ,Orringer MB,Iannettoni MD,Hanash SM,Beer DG

MA3-015 was used in immunohistochemistry to investigate the expression of heat shock protein 27 in normal and malignant cells

Mon Feb 01 00:00:00 EST 1999

Heat shock proteins hsp27 and hsp70: lack of correlation with response to tamoxifen and clinical course of disease in estrogen receptor-positive metastatic breast cancer (a Southwest Oncology Group Study).

Clinical cancer research : an official journal of the American Association for Cancer Research

Ciocca DR,Green S,Elledge RM,Clark GM,Pugh R,Ravdin P,Lew D,Martino S,Osborne CK

MA3-015 was used in immunohistochemistry to study whether hsp27 and hsp70 expression is correlated with clinical resistance to tamoxifen in ER-positive metastatic breast cancer

Fri May 01 00:00:00 EDT 1998

Expression of heat shock proteins in squamous cell carcinoma of the tongue: an immunohistochemical study.

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

Ito T,Kawabe R,Kurasono Y,Hara M,Kitamura H,Fujita K,Kanisawa M

MA3-015 was used in immunohistochemistry to investigate the distribution of heat shock protein proteins in squamous cell carcinoma of the tongue

Thu Jan 01 00:00:00 EST 1998

Chromosomal assignments of human 27-kDa heat shock protein gene family.

Somatic cell and molecular genetics

McGuire SE,Fuqua SA,Naylor SL,Helin-Davis DA,McGuire WL

MA3-015 was used in ELISA to identify the chromosomal location of hsp27

Wed Mar 01 00:00:00 EST 1989

A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells.

Breast cancer research and treatment

Moretti-Rojas I,Fuqua SA,Montgomery RA,McGuire WL

MA3-015 was used in immunoprecipitation to characterize the estradiol-regulated 24K protein in cancer

Sun May 01 00:00:00 EDT 1988

A new marker of maturation in the cervix: the estrogen-regulated 24K protein.

Obstetrics and gynecology

Dressler LG,Ramzy I,Sledge GW,McGuire WL

MA3-015 was used in immunohistochemistry to investigate hsp27 as a new biomarker for squamous cell maturation in the cervix

Mon Dec 01 00:00:00 EST 1986

Quantitative enzyme-linked immunosorbent assay for the estrogen-regulated Mr 24,000 protein in human breast tumors: correlation with estrogen and progesterone receptors.

Cancer research

Adams DJ,McGuire WL

MA3-015 was used in ELISA to investigate p24's expression level in human breast tumors and the relationship with estrogen and progesterone receptors

Sat Jun 01 00:00:00 EDT 1985

Detection of a Mr 24,000 estrogen-regulated protein in human breast cancer by monoclonal antibodies.

Cancer research

Adams DJ,Hajj H,Edwards DP,Bjercke RJ,McGuire WL

MA3-015 was used in immunoprecipitation to evaluate an antibody for detecting an estrogen-regulated 24 kDa protein

Thu Sep 01 00:00:00 EDT 1983

Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle.

The Journal of clinical endocrinology and metabolism

Ciocca DR,Asch RH,Adams DJ,McGuire WL

MA3-015 was used in immunohistochemistry to investigate the expression and localization of 24K protein in the endometrium of regularly cycling women

Thu Sep 01 00:00:00 EDT 1983

Bioinformatics

Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 27 kDa beta-1; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27

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Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792

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Entrez Gene ID: (Dog) 403979, (Human) 3315, (Rat) 24471

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Function(s)

identical protein binding protein homodimerization activity protein binding involved in protein folding protein kinase C binding protein binding protein kinase C inhibitor activity protein kinase binding ubiquitin binding poly(A) RNA binding

Process(es)

regulation of protein phosphorylation chaperone-mediated protein folding anterograde axonal protein transport retina homeostasis regulation of translational initiation negative regulation of protein kinase activity movement of cell or subcellular component response to unfolded protein response to virus regulation of autophagy positive regulation of interleukin-1 beta production intracellular signal transduction cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of tumor necrosis factor biosynthetic process negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling regulation of mRNA stability positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis vascular endothelial growth factor receptor signaling pathway platelet aggregation negative regulation of protein serine/threonine kinase activity negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of endothelial cell chemotaxis

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HSP27 Monoclonal Antibody (G3.1)

HSP27 Antibody (MA3-015) in ICC/IF

Product Details

MA3-015

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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