Proteintech

HSP60 Monoclonal Antibody (2F10E7)

HSP60 Antibody in Immunocytochemistry (ICC/IF)

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HSP60 Antibody (66041-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (Product # 66041-1-Ig) (Clone: 2F10E7) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

HSP60 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP60 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66041-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:2000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 9 publications 9 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F10E7

Immunogen

HSP60 Fusion Protein Ag7848 (225-573 aa encoded by BC003030)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.95 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SPYFINTSK GQKCEFQDAY VLLSEKKISS IQSIVPALEI ANAHRKPLVI IAEDVDGEAL STLVLNRLKV GLQVVAVKAP GFGDNRKNQL KDMAIATGGA VFGEEGLTLN LEDVQPHDLG KVGEVIVTKD DAMLLKGKGD KAQIEKRIQE IIEQLDVTTS EYEKEKLNER LAKLSDGVAV LKVGGTSDVE VNEKKDRVTD ALNATRAAVE EGIVLGGGCA LLRCIPALDS LTPANEDQKI GIEIIKRTLK IPAMTIAKNA GVEGSLIVEK IMQSSSEVGY DAMAGDFVNM VEKGIIDPTK VVRTALLDAA GVASLLTTAE VVVTEIPKEE KDPGMGAMGG MGGGMGGGMF (225-573 aa encoded by BC003030)

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Fukuda T,Furukawa K,Maruyama T,Yamashita SI,Noshiro D,Song C,Ogasawara Y,Okuyama K,Alam JM,Hayatsu M,Saigusa T,Inoue K,Ikeda K,Takai A,Chen L,Lahiri V,Okada Y,Shibata S,Murata K,Klionsky DJ,Noda NN,Kanki T

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Mon May 01 00:00:00 EDT 2023

Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation.

Diabetologia

Weng SW,Wu JC,Shen FC,Chang YH,Su YJ,Lian WS,Tai MH,Su CH,Chuang JH,Lin TK,Liou CW,Chu TH,Kao YH,Wang FS,Wang PW

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Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation.

Diabetologia

Weng SW,Wu JC,Shen FC,Chang YH,Su YJ,Lian WS,Tai MH,Su CH,Chuang JH,Lin TK,Liou CW,Chu TH,Kao YH,Wang FS,Wang PW

Mon May 01 00:00:00 EDT 2023

Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation.

Diabetologia

Weng SW,Wu JC,Shen FC,Chang YH,Su YJ,Lian WS,Tai MH,Su CH,Chuang JH,Lin TK,Liou CW,Chu TH,Kao YH,Wang FS,Wang PW

Mon May 01 00:00:00 EDT 2023

Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage.

Autophagy

Zhang Y,Fang Q,Wang H,Qi J,Sun S,Liao M,Wu Y,Hu Y,Jiang P,Cheng C,Qian X,Tang M,Cao W,Xiang S,Zhang C,Yang J,Gao X,Ying Z,Chai R

Sun Jan 01 00:00:00 EST 2023

Substitution of PINK1 Gly411 modulates substrate receptivity and turnover.

Autophagy

Fiesel FC,Fričová D,Hayes CS,Coban MA,Hudec R,Bredenberg JM,Broadway BJ,Markham BN,Yan T,Boneski PK,Fiorino G,Watzlawik JO,Hou X,McCarty AM,Lewis-Tuffin LJ,Zhong J,Madden BJ,Ordureau A,An H,Puschmann A,Wszolek ZK,Ross OA,Harper JW,Caulfield TR,Springer W

Thu Jun 01 00:00:00 EDT 2023

Selenoproteins synergistically protect porcine skeletal muscle from oxidative damage via relieving mitochondrial dysfunction and endoplasmic reticulum stress.

Journal of animal science and biotechnology

Jing J,He Y,Liu Y,Tang J,Wang L,Jia G,Liu G,Chen X,Tian G,Cai J,Che L,Kang B,Zhao H

Sun Jun 04 00:00:00 EDT 2023

Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis.

Redox biology

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Sat Jul 01 00:00:00 EDT 2023

R17C Mutation in Photoreceptor Disc-Specific Protein, PRCD, Results in Additional Lipidation Altering Protein Stability and Subcellular Localization.

International journal of molecular sciences

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Fri Sep 16 00:00:00 EDT 2022

Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival.

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Wed Jun 01 00:00:00 EDT 2022

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