Invitrogen

HSP60 Monoclonal Antibody (A57-B9)

HSP60 Antibody in Immunocytochemistry (ICC/IF)

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HSP60 Antibody (MA3-023) in ICC/IF

PloS one 2014 - Figure 1 Peroxisomes are translocated into the Chlamydia inclusion during infection. A- HeLa cells were infected with C. trachomatis L2 for 20 h. The inclusion membrane was labeled with an anti-CT813 antibody (green), peroxisomes with an anti-ALDP antibody (red) and bacterial and nuclear DNA with Hoechst (Blue). A single ApoTome x-y section is shown in the central image. The z-x projection on the top shows ... View More

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Product Details

MA3-023

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A57-B9

Immunogen

Recombinant Chlamydia trachomatis HSP60 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325470

Product Specific Information

MA3-023 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects all three species of Chlamydia HSP60 with minimal cross-reactivity with Borrelia burgdorferi HSP60. MA3-023 does not react with E. coli GroEL.

MA3-023 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~60 kDa band representing HSP60 from C. trachomatis lysate.

The MA3-023 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of Chlamydia HSP60 amino acids 517-522.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Host Cell Amplification of Nutritional Stress Contributes To Persistence in Chlamydia trachomatis.

mBio

Pokorzynski ND,Alla MR,Carabeo RA

MA3-023 was used in Immunocytochemistry to report that during starvation of the essential nutrients iron or tryptophan, a common response of the infected epithelial cell is the suppression of GTP biosynthesis, which induces a persistent developmental state in the pathogen.

Tue Dec 20 00:00:00 EST 2022

Chlamydia trachomatis development requires both host glycolysis and oxidative phosphorylation but has only minor effects on these pathways.

The Journal of biological chemistry

N'Gadjaga MD,Perrinet S,Connor MG,Bertolin G,Millot GA,Subtil A

MA3-023 was used in Flow cytometry/Cell sorting to conclude that ATP consumption appears at equilibrium with the normal production capacity of the host and the bacteria, so that no major shift between these pathways is required to meet bacterial needs.

Thu Sep 01 00:00:00 EDT 2022

The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis.

Nature communications

Pokorzynski ND,Hatch ND,Ouellette SP,Carabeo RA

MA3-023 was used in Western Blot, Immunocytochemistry to conclude that repurposing the iron-dependent repressor YtgR underscores the fundamental importance of maintaining tryptophan-dependent attenuation of the trpRBA operon.

Tue Dec 22 00:00:00 EST 2020

The iron-dependent repressor YtgR is a tryptophan-dependent attenuator of the trpRBA operon in Chlamydia trachomatis.

Nature communications

Pokorzynski ND,Hatch ND,Ouellette SP,Carabeo RA

Tue Dec 22 00:00:00 EST 2020

Chlamydia trachomatis recruits protein kinase C during infection.

Pathogens and disease

Sah P,Nelson NH,Shaw JH,Lutter EI

Published figure using HSP60 monoclonal antibody (Product # MA3-023) in Western Blot

Thu Aug 01 00:00:00 EDT 2019

The Loss of Expression of a Single Type 3 Effector (CT622) Strongly Reduces Chlamydia trachomatis Infectivity and Growth.

Frontiers in cellular and infection microbiology

Cossé MM,Barta ML,Fisher DJ,Oesterlin LK,Niragire B,Perrinet S,Millot GA,Hefty PS,Subtil A

MA3-023 was used in Immunocytochemistry-immunoflourescence to identify CT622 as a secreted protein that plays multiple and crucial roles in the initiation and support of the C. trachomatis growth cycle.

Mon May 20 00:00:00 EDT 2019

Targeted Disruption of Chlamydia trachomatis Invasion by in Trans Expression of Dominant Negative Tarp Effectors.

Frontiers in cellular and infection microbiology

Parrett CJ,Lenoci RV,Nguyen B,Russell L,Jewett TJ

MA3023 was used in western blot to study the role of Tarp during Chlamydia trachomatis's invasion of eukaryotic host cells

Fri Sep 22 00:00:00 EDT 2017

Chlamydia Infection Across Host Species Boundaries Promotes Distinct Sets of Transcribed Anti-Apoptotic Factors.

Frontiers in cellular and infection microbiology

Messinger JE,Nelton E,Feeney C,Gondek DC

MA3-023 was used in immunocytochemistry to research promotion of distinct sets of transcribed anti-apoptotic factors due to chlamydia infection across host species boundaries

Tue Jun 14 00:00:00 EDT 2016

The intracellular bacteria Chlamydia hijack peroxisomes and utilize their enzymatic capacity to produce bacteria-specific phospholipids.

PloS one

Boncompain G,Müller C,Meas-Yedid V,Schmitt-Kopplin P,Lazarow PB,Subtil A

MA3-023 was used in immunocytochemistry to study the synthesis of bacteria-specific phospholipids in peroxisomes of cells infected with the intracellular bacterial pathogen Chlamydia

Tue Nov 11 00:00:00 EST 2014

Chlamydophila pneumoniae infection leads to smooth muscle cell proliferation and thickening in the coronary artery without contributions from a host immune response.

The American journal of pathology

Deniset JF,Cheung PK,Dibrov E,Lee K,Steigerwald S,Pierce GN

MA3-023 was used in western blot to study the induction of smooth muscle cell proliferation and vascular thickening during Chlamydophila pneumoniae infection

Mon Feb 01 00:00:00 EST 2010

Association of carotid plaque Lp-PLA(2) with macrophages and Chlamydia pneumoniae infection among patients at risk for stroke.

PloS one

Atik B,Johnston SC,Dean D

MA3-023 was used in immunohistochemistry to investigate the interplay among lipoprotein-associated phospholipase A2, macrophages, and Chlamydia pneumoniae infection in atherosclerosis

Wed Jun 09 00:00:00 EDT 2010

Matrix metalloproteinase-9 expression is associated with the presence of Chlamydia pneumoniae in human coronary atherosclerotic plaques.

Heart (British Cardiac Society)

Arno G,Kaski JC,Smith DA,Akiyu JP,Hughes SE,Baboonian C

MA3-023 was used in immunohistochemistry to study the relationship between MMP-9 expression and the presence of Chlamydia pneumoniae in atherosclerotic plaques

Fri Apr 01 00:00:00 EST 2005

Chlamydia pneumoniae infection in circulating human monocytes is refractory to antibiotic treatment.

Circulation

Gieffers J,Füllgraf H,Jahn J,Klinger M,Dalhoff K,Katus HA,Solbach W,Maass M

MA3-023 was used in immunocytochemistry to investigate the influence of rifampin and azithromycin on chlamydial growth and activity within monocytes

Tue Jan 23 00:00:00 EST 2001

Chlamydia pneumoniae in abdominal aortic aneurysms: abundance of membrane components in the absence of heat shock protein 60 and DNA.

Arteriosclerosis, thrombosis, and vascular biology

Meijer A,van Der Vliet JA,Roholl PJ,Gielis-Proper SK,de Vries A,Ossewaarde JM

MA3-023 was used in immunohistochemistry to detect chlamydia pneumoniae in abdominal aortic aneurysms

Mon Nov 01 00:00:00 EST 1999

Tryptophan depletion as a mechanism of gamma interferon-mediated chlamydial persistence.

Infection and immunity

Beatty WL,Belanger TA,Desai AA,Morrison RP,Byrne GI

MA3-023 was used in western blot to investigate the role of gamma interferon in chlamydial persistence

Thu Sep 01 00:00:00 EDT 1994

Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro.

Proceedings of the National Academy of Sciences of the United States of America

Beatty WL,Byrne GI,Morrison RP

MA3-023 was used in immunocytochemistry to investigate the effect of gamma interferon on Chlamydia trachomatis infection

Sat May 01 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: 60 kDa heat shock protein; Heat shock protein; HSP; Hsp60s1

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HSP60 Monoclonal Antibody (A57-B9)

HSP60 Antibody (MA3-023) in ICC/IF

Product Details

MA3-023

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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