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HSP60 Antibody (MA5-45114) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Hsp60 monoclonal antibody (Product # MA5-45114) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
Product Details
MA5-45114
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria,
Bovine,
Dog,
Chicken,
Fish,
Guinea pig,
Hamster,
Human,
Insect,
Mouse,
Non-human primate,
Nematode,
Plant,
Pig,
Rabbit,
Rat,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LK2
Immunogen
Recombinant human HSP60
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.5mM phosphate/2.3mM MES with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931568
Product Specific Information
0.25 µg/mL of MA5-45114 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.|Detects approximately 60kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; chaperonin; Chaperonin 60; CPN60; heat shock 60 kDa protein 1; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); Heat shock protein 60, mitochondrial; heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; hsp60; Hsp60s1; HuCHA60; mitochondrial heat shock 60 kDa protein 1; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; P66; short heat shock protein 60 Hsp60s1; Stimulator factor I 66 kDa component
Gene Aliases: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; I79_011398; L8479.10; MIF4; MNA2; RCJMB04_7g5; SPG13; YLR259C
nucleotide binding
DNA replication origin binding
single-stranded DNA binding
ATP binding
ATPase activity
unfolded protein binding
chaperone binding
ATP-dependent protein folding chaperone
isomerase activity
lipopolysaccharide binding
p53 binding
double-stranded RNA binding
protein binding
high-density lipoprotein particle binding
ubiquitin protein ligase binding
apolipoprotein A-I binding
poly(A) RNA binding
protease binding
protein complex binding
insulin binding
protein heterodimerization activity
misfolded protein binding
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
apoptotic mitochondrial changes
response to cold
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
mitochondrial unfolded protein response
protein refolding
B cell proliferation
T cell activation
positive regulation of macrophage activation
negative regulation of apoptotic process
protein import into mitochondrial intermembrane space
isotype switching to IgG isotypes
protein stabilization
positive regulation of T cell activation
interaction with symbiont
cellular response to interleukin-7
mitochondrial genome maintenance
'de novo' protein folding
chaperone-mediated protein complex assembly
protein maturation
detection of misfolded protein
response to unfolded protein
B cell activation
positive regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of inflammatory response
chaperone mediated protein folding requiring cofactor
viral process
response to hypoxia
response to heat
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
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