Invitrogen

HSP70 Monoclonal Antibody (2A4)

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

1 Published Figure
11 References
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Datasheet
Protocols
Questions & Answers

Product Details

MA3-008

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Immunoprecipitation (IP)

2 µg
-

Neutralization (Neu)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Amphibian, Avian, Fruit fly, Fish, Human, Mouse, Rat, Yeast

Published species

Fish, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

2A4

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325456

Product Specific Information

MA3-008 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.

MA3-008 has been successfully used in Western blot, immunohistochemistry (paraffin), immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~72 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunofluorescence staining of HSP70 in heat shocked HeLa cells with MA3-008 results in cytoplasmic staining.

The MA3-008 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 437-479 of human HSP70.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kilodalton heat shock protein; 87A locus; 87C locus; CG31359-PA; CG31366-PA; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 70; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein alpha 2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; heat-shock protein 70; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-87A7; HSP70-87C1; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; Hsp70Aa-PA; Hsp70Bb-PA; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; protein 70; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70

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Gene Aliases: 70kDa; 87A; AI317151; APG-2; APG2; Bb; CG18743; CG31359; CG31366; CG31449; CG6489; dHSP70; dhsp70Aa; Dm-hsp70; Dmel\CG31359; Dmel\CG31366; Dmel_CG31359; Dmel_CG31366; DmHSP70AA; DMHSP7D1; Hcp70.1; Hcp70.2; HEL-S-103; HEL-S-5a; HS24/P52; hs70; Hsc70t; HSP 70; Hsp-70; Hsp-70Aa; Hsp110; hsp68; HSP70; hsp70 87A; hsp70 87C; hsp70 Aa; hsp70(87A); Hsp70(87C); HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A; Hsp70A1; HSP70A2; Hsp70A7d; Hsp70Aa; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bc; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; Hspt70; Hst70; HSX70; hum70t; Irp94; mKIAA4025; Msh5; RY

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UniProt ID: (Human) P0DMV8, (Human) P34931, (Human) P54652, (Human) P34932, (Fruit fly) P02825, (Fruit fly) Q9BIR7, (Mouse) Q61696, (Rat) P0DMW0, (Mouse) Q61689, (Rat) P55063, (Mouse) P16627, (Rat) P14659, (Mouse) P17156, (Rat) O88600

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Entrez Gene ID: (Human) 3303, (Human) 3304, (Human) 3305, (Human) 3306, (Human) 3308, (Fruit fly) 48581, (Fruit fly) 48582, (Mouse) 193740, (Rat) 24472, (Rat) 294254, (Mouse) 15511, (Rat) 24963, (Mouse) 15482, (Rat) 60460, (Mouse) 15512, (Mouse) 15525, (Rat) 266759

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Function(s)
virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion glycolipid binding misfolded protein binding protease binding double-stranded RNA binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding molecular_function nucleotide binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding
Process(es)
response to unfolded protein male meiosis male meiosis I spermatogenesis spermatid development response to heat response to cold positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle protein refolding synaptonemal complex disassembly negative regulation of inclusion body assembly positive regulation of calcium-transporting ATPase activity positive regulation of gene expression regulation of cell death regulation of protein ubiquitination negative regulation of protein ubiquitination positive regulation of interleukin-8 production cellular response to oxidative stress cellular response to heat ATP metabolic process viral entry into host cell protein stabilization positive regulation of NF-kappaB transcription factor activity negative regulation of cell death cellular heat acclimation positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway regulation of cellular response to heat positive regulation of tumor necrosis factor-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand binding of sperm to zona pellucida positive regulation of protein targeting to mitochondrion biological_process telomere maintenance DNA repair protein folding regulation of mRNA stability cell-cell adhesion negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoribonuclease activity positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response response to hypoxia vesicle-mediated transport cellular response to unfolded protein heat shock-mediated polytene chromosome puffing chaperone mediated protein folding requiring cofactor negative regulation of protein phosphorylation negative regulation of gene expression positive regulation of protein binding negative regulation of apoptotic process negative regulation of DNA binding protein import into mitochondrial outer membrane chaperone-mediated protein complex assembly response to ischemia mRNA catabolic process negative regulation of cell proliferation response to radiation response to mechanical stimulus neuron differentiation negative regulation of cell growth response to ethanol positive regulation of erythrocyte differentiation positive regulation of neuron differentiation kidney development positive regulation of angiogenesis positive regulation of protein phosphorylation multicellular organism development positive regulation of T cell mediated cytotoxicity defense response negative regulation of myeloid cell apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of vasoconstriction cell differentiation
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