Invitrogen

HSP70 Monoclonal Antibody (5A5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

49 References

View all (60) HSP70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HSP70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

HSP70 Antibody (MA3-007) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 70 (HSP70) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSP70 Monoclonal Antibody (Product # MA3-007), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:4... View More

HSP70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP70 Antibody in Immunoprecipitation (IP)

HSP70 Antibody in Immunohistochemistry (IHC)

HSP70 Antibody in Functional Assay, T-Cell Activation (Functional, TCA)

Product Image Gallery

HSP70 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using HSP70 Mouse Monoclonal Antibody (5A5) (Product # MA3-007), shows increased expression of proteins upon heat shock treatment. {TM}

Product Details

MA3-007

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 36 publications 36 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:200

View 2 publications 2 publications

ELISA (ELISA)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 1 publication 1 publication

T-Cell Activation (TCA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Amphibian, Avian, Dog, Fruit fly, Fish, Flatworm, Human, Mouse, Non-human primate, Rat, Yeast

Published species

Amphibian, Avian, Bovine, Fruit fly, Human, Mollusc, Mouse, Rabbit, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5A5

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325455

Product Specific Information

MA3-007 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, GRP78, and following heat shock, HSP72 from yeast, rat, Drosophila, fish, mouse, avian, amphibian and human samples.

MA3-007 has been successfully used in Western blot, immunofluorescence, gel shift, immunohistochemistry (frozen), and immunoprecipitation procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunofluorescence staining of HSP70 in heat shocked HeLa cells with MA3-007 results in cytoplasmic staining.

The MA3-007 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 122-264 of human HSP70, a region that has been shown to be involved in ATP binding. This is the first monoclonal antibody reported to react with: 1) the ATP binding region of HSP70, 2) an epitope in the amino terminus of HSP70.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Secretagogin is a Ca²⁺-dependent stress-responsive chaperone that may also play a role in aggregation-based proteinopathies.

The Journal of biological chemistry

Chidananda AH,Khandelwal R,Jamkhindikar A,Pawar AD,Sharma AK,Sharma Y

MA3-007 was used in Western Blotting to report that Secretagogin SCGN is an early responder to cellular stress, and SCGN expression is temporally upregulated by oxidative stress and heat shock.

Thu Sep 01 00:00:00 EDT 2022

Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR-Cas13d in mammalian cells.

Cell reports

Kelley CP,Haerle MC,Wang ET

MA3-007 was used in Western Blotting to find that, when targeting CUGn RNA in mammalian cells, Cas13d depletes endogenous and transgenic RNAs, interferes with critical cellular processes, and activates stress response and apoptosis.

Tue Aug 16 00:00:00 EDT 2022

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

PLoS genetics

Gersing SK,Wang Y,Grønbæk-Thygesen M,Kampmeyer C,Clausen L,Willemoës M,Andréasson C,Stein A,Lindorff-Larsen K,Hartmann-Petersen R

MA3-007 was used in Western Blotting to investigate the degradation pathway of a disease-causing protein variant, using ASPA C152W as a model.

Thu Apr 01 00:00:00 EDT 2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

PLoS genetics

Gersing SK,Wang Y,Grønbæk-Thygesen M,Kampmeyer C,Clausen L,Willemoës M,Andréasson C,Stein A,Lindorff-Larsen K,Hartmann-Petersen R

MA3-007 was used in Western Blotting to investigate the degradation pathway of a disease-causing protein variant, using ASPA C152W as a model.

Thu Apr 01 00:00:00 EDT 2021

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

MA3-007 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to identify liquid biopsy biomarkers for patients with high risk of bone metastasis.

Tue Aug 31 00:00:00 EDT 2021

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

Tue Aug 31 00:00:00 EDT 2021

75-kDa glucose-regulated protein (GRP75) is a novel molecular signature for heat stress response in avian species.

American journal of physiology. Cell physiology

Dhamad AE,Greene E,Sales M,Nguyen P,Beer L,Liyanage R,Dridi S

MA3-007 was used in Western Blotting to identify chicken GRP75 by using immunoprecipitation assay integrated with LC-MS/MS, and found that its amino acid sequence is conserved with high homology (52.5%) to the HSP70 family.

Sat Feb 01 00:00:00 EST 2020

Expression of Heat Shock Protein 70 Is Insufficient To Extend Drosophila melanogaster Longevity.

G3 (Bethesda, Md.)

Xiao C,Hull D,Qiu S,Yeung J,Zheng J,Barwell T,Robertson RM,Seroude L

MA3-007 was used in Western Blotting to determine whether hsp70 is sufficient to affect the longevity and the resistance to thermal, oxidative or desiccation stresses of the whole organism.

Tue Dec 03 00:00:00 EST 2019

Prognostic value of histone chaperone FACT subunits expression in breast cancer.

Breast cancer (Dove Medical Press)

Attwood K,Fleyshman D,Prendergast L,Paszkiewicz G,Omilian AR,Bshara W,Gurova K

MA3-007 was used in Western Blotting to characterise the pattern of FACT expression in breast cancer, in relation to other molecular and clinical prognostic markers.

Thu Oct 01 00:00:00 EDT 2020

Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs.

The Journal of experimental biology

Ivanina AV,Nesmelova I,Leamy L,Sokolov EP,Sokolova IM

MA3-007 was used in western blot to determine the affect of cellular bioenergetics in marine molluscs and functional reorganization of mitochondria by intermittent hypoxia

Wed Jun 01 00:00:00 EDT 2016

Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation.

PloS one

Mutschelknaus L,Peters C,Winkler K,Yentrapalli R,Heider T,Atkinson MJ,Moertl S

MA3-007 was used in western blot to calculate how ionizing radiation can promote cell survival in exosomes derived from squamous head and neck cancer

Tue Aug 02 00:00:00 EDT 2016

Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction.

Journal of proteome research

Azimzadeh O,Sievert W,Sarioglu H,Merl-Pham J,Yentrapalli R,Bakshi MV,Janik D,Ueffing M,Atkinson MJ,Multhoff G,Tapio S

MA3-007 was used in western blot to elucidate the factors that mediate radiation-induced endothelial dysfunction.

Fri Feb 06 00:00:00 EST 2015

Temperature-dependent toxicities of nano zinc oxide to marine diatom, amphipod and fish in relation to its aggregation size and ion dissolution.

Nanotoxicology

Wong SW,Leung KM

MA3-007 was used in western blot to study the toxicity of nano zinc oxide particles to selected marine organisms

Fri Aug 01 00:00:00 EDT 2014

Autophagic degradation of the inhibitory p53 isoform Δ133p53α as a regulatory mechanism for p53-mediated senescence.

Nature communications

Horikawa I,Fujita K,Jenkins LM,Hiyoshi Y,Mondal AM,Vojtesek B,Lane DP,Appella E,Harris CC

MA3-007 was used in Western Blotting to identify a p53 isoform-specific protein turnover mechanism that orchestrates p53-mediated senescence.

Thu Aug 21 00:00:00 EDT 2014

Charcot-Marie-Tooth disease CMT4A: GDAP1 increases cellular glutathione and the mitochondrial membrane potential.

Human molecular genetics

Noack R,Frede S,Albrecht P,Henke N,Pfeiffer A,Knoll K,Dehmel T,Meyer Zu Hörste G,Stettner M,Kieseier BC,Summer H,Golz S,Kochanski A,Wiedau-Pazos M,Arnold S,Lewerenz J,Methner A

MA3-007 was used in western blot to study the effect of a GDAP1 mutation on cellular glutathione and mitochondrial membrane potential

Sun Jan 01 00:00:00 EST 2012

Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis.

Journal of evolutionary biology

Calabria G,Dolgova O,Rego C,Castañeda LE,Rezende EL,Balanyà J,Pascual M,Sørensen JG,Loeschcke V,Santos M

MA3-007 was used in ELISA to test the thermal co-adaptation hypothesis using Drosophila subobscura

Sun Apr 01 00:00:00 EDT 2012

Shotgun proteomics analysis reveals new unsuspected molecular effectors of nitrogen-containing bisphosphonates in osteocytes.

Journal of proteomics

Bivi N,Picotti P,Müller LN,Romanello M,Moro L,Quadrifoglio F,Tell G

MA3-007 was used in western blot to investigate the effect of nitrogen-containing bisphosphonate treatment in murine osteocytes

Fri Jun 10 00:00:00 EDT 2011

Thermal adaptation in the intertidal snail Echinolittorina malaccana contradicts current theory by revealing the crucial roles of resting metabolism.

The Journal of experimental biology

Marshall DJ,Dong YW,McQuaid CD,Williams GA

MA3-007 was used in western blot to investigate the involvement of resting metabolism in thermal adaptation theory

Tue Nov 01 00:00:00 EDT 2011

Assay conditions in laboratory experiments: is the use of constant rather than fluctuating temperatures justified when investigating temperature-induced plasticity?

Oecologia

Fischer K,Kölzow N,Höltje H,Karl I

MA3007 was used in ELISA to evaluate if the use of constant temperatures is justified when studying Lycaena tityrus

Sun May 01 00:00:00 EDT 2011

Thermal constraints for range expansion of the invasive green mussel, Perna viridis, in the southeastern United States.

Journal of experimental zoology. Part A, Ecological genetics and physiology

Urian AG,Hatle JD,Gilg MR

MA3-007 was used in western blot to investigate the effect of temperature on the expansion of an invasive mussel species

Sat Jan 01 00:00:00 EST 2011

Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.

Cell death and differentiation

Nisoli I,Chauvin JP,Napoletano F,Calamita P,Zanin V,Fanto M,Charroux B

MA3-007 was used in immunohistochemistry to investigate the effect of autophagy in the pathogenesis of dentatorubral-pallidoluysian Atrophy

Fri Oct 01 00:00:00 EDT 2010

Elevated expression of Foxp3 in tumor-infiltrating Treg cells suppresses T-cell proliferation and contributes to gastric cancer progression in a COX-2-dependent manner.

Clinical immunology (Orlando, Fla.)

Yuan XL,Chen L,Li MX,Dong P,Xue J,Wang J,Zhang TT,Wang XA,Zhang FM,Ge HL,Shen LS,Xu D

MA3-007 was used in western blot to study the Foxp3 expression in tumor-infiltrating Treg cells and its effect on T-cell proliferation and gastric cancer progression

Mon Mar 01 00:00:00 EST 2010

Neuroprotection resulting from insufficiency of RANBP2 is associated with the modulation of protein and lipid homeostasis of functionally diverse but linked pathways in response to oxidative stress.

Disease models & mechanisms

Cho KI,Yi H,Tserentsoodol N,Searle K,Ferreira PA

MA3-007 was used in immunocytochemistry to investigate the neuroprotective effect of RANBP2 insufficiency against light damage

Mon Dec 27 00:00:00 EST 2010

Effect of progerin on the accumulation of oxidized proteins in fibroblasts from Hutchinson Gilford progeria patients.

Mechanisms of ageing and development

Viteri G,Chung YW,Stadtman ER

MA3-007 was used in western blot to investigate the influence of progerin on oxidative stress responses in normal fibrblasts and those from Hutchinson Gilford progeria patients

Fri Jan 01 00:00:00 EST 2010

Toxicities of nano zinc oxide to five marine organisms: influences of aggregate size and ion solubility.

Analytical and bioanalytical chemistry

Wong SW,Leung PT,Djurisić AB,Leung KM

MA3-007 was used in western blot to study the toxic effect of nano zinc oxide on marine organisms and its mechanism

Fri Jan 01 00:00:00 EST 2010

Accumulation of neoplastic traits prior to spontaneous in vitro transformation of rat cholangiocytes determines susceptibility to activated ErbB-2/Neu.

Experimental and molecular pathology

Rozich RA,Mills DR,Brilliant KE,Callanan HM,Yang D,Tantravahi U,Hixson DC

MA3-007 was used in western blot to study the in vitro transformation of rat cholangiocytes

Wed Dec 01 00:00:00 EST 2010

Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis.

Biochemical and biophysical research communications

Paoletti F,Ainger K,Donati I,Scardigli R,Vetere A,Cattaneo A,Campa C

MA3-007 was used in immunocytochemistry to evaluate the novel fluorescent derivatives of protein synthesis studcycloheximide as reporters for cellular function study

Fri May 28 00:00:00 EDT 2010

The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.

PLoS genetics

Juge F,Fernando C,Fic W,Tazi J

MA3-007 was used in western blot to study the role of SR protein B52/SRp55 in the recruitment of DNA topoisomerase I to genomic activities

Thu Sep 16 00:00:00 EDT 2010

Characterization of the properties of a novel mutation in VAPB in familial amyotrophic lateral sclerosis.

The Journal of biological chemistry

Chen HJ,Anagnostou G,Chai A,Withers J,Morris A,Adhikaree J,Pennetta G,de Belleroche JS

MA3-007 was used in immunohistochemistry to investigate the role of a vesicle-associated membrane protein-associated protein B mutation in the pathology of familial amyotrophic lateral sclerosis

Fri Dec 17 00:00:00 EST 2010

Effects of elevated temperature and cadmium exposure on stress protein response in eastern oysters Crassostrea virginica (Gmelin).

Aquatic toxicology (Amsterdam, Netherlands)

Ivanina AV,Taylor C,Sokolova IM

MA3-007 was used in western blot to investigate the influence of temperature and cadmium on protein expression in oysters

Thu Feb 19 00:00:00 EST 2009

Surface expression of a C-terminal alpha-helix region in heat shock protein 72 on murine LL/2 lung carcinoma can be recognized by innate immune sentinels.

Molecular immunology

Tani F,Ohno M,Furukawa Y,Sakamoto M,Masuda S,Kitabatake N

MA3-007 was used in western blot to study the induction of heat shock protein 72 expression in murine LL/2 lung carcinoma

Wed Apr 01 00:00:00 EDT 2009

Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin.

The Journal of experimental biology

Ivanina AV,Cherkasov AS,Sokolova IM

MA3-007 was used in western blot to investigate the effects of cadmium exposure on expression of heat shock proteins (HSPs) in eastern oysters

Fri Feb 01 00:00:00 EST 2008

Oxidative stress and expression of chaperones in aging mollusks.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Ivanina AV,Sokolova IM,Sukhotin AA

MA3-007 was used in western blot to investigate the age-dependent oxidative stress and heat shock response in mollusks

Thu May 01 00:00:00 EDT 2008

A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1.

The EMBO journal

Chartier A,Benoit B,Simonelig M

MA3-007 was used in immunohistochemistry to study the intrinsic toxicity of PABPN1 in a Drosophila model of oculopharyngeal muscular dystrophy

Wed May 17 00:00:00 EDT 2006

Heat shock-mediated thermoprotection of larval locomotion compromised by ubiquitous overexpression of Hsp70 in Drosophila melanogaster.

Journal of neurophysiology

Klose MK,Chu D,Xiao C,Seroude L,Robertson RM

MA3-007 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice

Tue Nov 01 00:00:00 EST 2005

Synaptic thermoprotection in a desert-dwelling Drosophila species.

Journal of neurobiology

Newman AE,Xiao C,Robertson RM

MA3-007 was used in western blot to investigate the involvement of heat shock proteins in synaptic thermoprotection

Mon Aug 01 00:00:00 EDT 2005

Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.

Reproductive biology and endocrinology : RB&E

Calvert ME,Digilio LC,Herr JC,Coonrod SA

MA3-007 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.

Fri Feb 14 00:00:00 EST 2003

Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper.

The EMBO journal

Thress K,Song J,Morimoto RI,Kornbluth S

MA3-007 was used in western blot to study the role of Scythe and Reaper in Hsp70 chaperone function.

Thu Mar 01 00:00:00 EST 2001

Proceedings of the National Academy of Sciences of the United States of America

Giraldo R,Diaz-Orejas R

MA3-007 was used in immunoprecipitation to investigate the evolutionary conservation of the DNA replication initiators between Gram-negative bacteria plasmids RepA and eukaryotes Orc4p.

Tue Apr 24 00:00:00 EDT 2001

Effect of ischemic pretreatment on heat shock protein 72, neurologic outcome, and histopathologic outcome in a rabbit model of spinal cord ischemia.

The Journal of thoracic and cardiovascular surgery

de Haan P,Vanicky I,Jacobs MJ,Bakker O,Lips J,Meylaerts SA,Kalkman CJ

MA3-007 was used in western blot to investigate the effect of ischemic pretreatment for HSP72 expression and neural damage in rabbit

Fri Sep 01 00:00:00 EDT 2000

Complex regulation of the yeast heat shock transcription factor.

Molecular biology of the cell

Bonner JJ,Carlson T,Fackenthal DL,Paddock D,Storey K,Lea K

MA3-007 was used in EMSA to investigate the regulatory roles of yeast heat shock transcription factor.

Mon May 01 00:00:00 EDT 2000

Complex regulation of the yeast heat shock transcription factor.

Molecular biology of the cell

Bonner JJ,Carlson T,Fackenthal DL,Paddock D,Storey K,Lea K

MA3-007 was used in EMSA to investigate the regulatory roles of yeast heat shock transcription factor.

Mon May 01 00:00:00 EDT 2000

Heat shock protein 70 (hsp70) as a major target of the antibody response in patients with pulmonary cryptococcosis.

Clinical and experimental immunology

Kakeya H,Udono H,Maesaki S,Sasaki E,Kawamura S,Hossain MA,Yamamoto Y,Sawai T,Fukuda M,Mitsutake K,Miyazaki Y,Tomono K,Tashiro T,Nakayama E,Kohno S

MA3-007 was used in western blot to demonstrate that heat shock protein 70 (hsp70) could be a major target molecule of the humoral response in patients with pulmonary cryptococcosis

Mon Mar 01 00:00:00 EST 1999

Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance.

The Journal of biological chemistry

Gabai VL,Meriin AB,Mosser DD,Caron AW,Rits S,Shifrin VI,Sherman MY

MA3-007 was used in blocking/activating experiment to investigate the role of Hsp70 in modulation of stress kinase activity

Fri Jul 18 00:00:00 EDT 1997

Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance.

The Journal of biological chemistry

Gabai VL,Meriin AB,Mosser DD,Caron AW,Rits S,Shifrin VI,Sherman MY

MA3-007 was used in blocking/activating experiment to investigate the role of Hsp70 in modulation of stress kinase activity

Fri Jul 18 00:00:00 EDT 1997

Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance.

The Journal of biological chemistry

Gabai VL,Meriin AB,Mosser DD,Caron AW,Rits S,Shifrin VI,Sherman MY

MA3-007 was used in blocking/activating experiment to investigate the role of Hsp70 in modulation of stress kinase activity

Fri Jul 18 00:00:00 EDT 1997

Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells.

The Journal of steroid biochemistry and molecular biology

van den Berg JD,Smets LA,van Rooij H

MA3-007 was used in western blot to study the role of cell-cell interactions in the ligand-independent nuclear translocation of the glucocorticoid receptor

Thu Feb 01 00:00:00 EST 1996

Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress.

Proceedings of the National Academy of Sciences of the United States of America

Wheeler JC,Bieschke ET,Tower J

MA3-007 was used in western blot to study the effect of aging and oxidative stress on the expression of HSP70 in Drosophila muscle

Tue Oct 24 00:00:00 EDT 1995

hsc70 moderates the heat shock (stress) response in Xenopus laevis oocytes and binds to denatured protein inducers.

The Journal of biological chemistry

Mifflin LC,Cohen RE

MA3-007 was used in western blot to study how hsc70 reduces the stress response in Xenopus oocytes

Fri Jun 03 00:00:00 EDT 1994

A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver.

FEBS letters

Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

MA3-007 was used in western blot to study the role of heat-shock protein 70 in the outer membranes of mitochondria from rat liver

Mon Oct 18 00:00:00 EDT 1993

Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni.

Infection and immunity

Richter D,Harn DA

MA3-007 was used in western blot to evaluate the vaccine antigen candidates for Schistosoma mansoni

Fri Jan 01 00:00:00 EST 1993

Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kilodalton heat shock protein; 87A locus; 87C locus; CG31359-PA; CG31366-PA; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 70; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein alpha 2; heat shock protein Apg-2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; heat-shock protein 70; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-87A7; HSP70-87C1; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; Hsp70Aa-PA; Hsp70Bb-PA; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; LP05203p; LP05233p; LP08776p; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; protein 70; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70

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Gene Aliases: 70kDa; 87A; AI317151; APG-2; APG2; Bb; CG18743; CG31359; CG31366; CG31449; CG6489; dHSP70; dhsp70Aa; Dm-hsp70; Dmel\CG31359; Dmel\CG31366; Dmel_CG31359; Dmel_CG31366; DmHSP70AA; DMHSP7D1; Hcp70.1; Hcp70.2; HEL-S-103; HEL-S-5a; HS24/P52; hs70; Hsc70t; HSP 70; Hsp-70; Hsp-70Aa; Hsp110; hsp68; HSP70; hsp70 87A; hsp70 87C; hsp70 Aa; hsp70(87A); Hsp70(87C); HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A; Hsp70A1; HSP70A2; Hsp70A7d; Hsp70Aa; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bc; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; Hspt70; Hst70; HSX70; hum70t; Irp94; mKIAA4025; Msh5; RY

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UniProt ID: (Dog) Q7YQC6, (Human) P0DMV8, (Human) P34931, (Human) P54652, (Dog) Q2TFN9, (Human) P34932, (Fruit fly) P02825, (Fruit fly) Q9BIR7, (Mouse) Q61696, (Rat) P0DMW0, (Mouse) Q61689, (Rat) P55063, (Mouse) P16627, (Rat) P14659, (Mouse) P17156, (Rat) O88600

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Function(s)

virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion glycolipid binding misfolded protein binding protease binding double-stranded RNA binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding molecular_function nucleotide binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding

Process(es)

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HSP70 Monoclonal Antibody (5A5)

HSP70 Antibody (MA3-007) in ICC/IF

Product Details

MA3-007

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Gel Shift (GS)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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