Invitrogen

HSP70 Monoclonal Antibody (W27)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

24 References
View all (60) HSP70 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-90504

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
View 12 publications 12 publications

Immunohistochemistry (IHC)

-
View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-dependent
View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent
-

Immunoprecipitation (IP)

2 µg/mg
-
Product Specifications

Species Reactivity

Bovine, Human, Non-human primate

Published species

Dog, Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

W27

Immunogen

HSP70 protein from HeLa cells.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1955811

Product Specific Information

Recommended positive controls: BT474 or HeLa cells. Breast carcinoma..

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 70 kda heat shock protein-2; 70 kilodalton heat shock protein; chaperone; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kD protein 3; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 3; heat shock 70kDa protein 4; Heat shock protein; Heat Shock Protein 70; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; HEL S 103; HSP; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70.3; HSP70RY; HSPA2; HSPA4; Hspa70

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Gene Aliases: APG-2; APG2; HEL-S-103; HEL-S-5a; HS24/P52; HSP70; HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; HSP70I; hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA3; HSPA4; HSPH2; HSX70; hum70t; RY

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UniProt ID: (Human) P0DMV8, (Bovine) Q27975, (Human) P34931, (Bovine) P0CB32, (Human) P54652, (Bovine) P34933, (Human) P34932

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Entrez Gene ID: (Human) 3303, (Bovine) 282254, (Human) 3304, (Human) 3305, (Bovine) 540190, (Human) 3306, (Bovine) 281827, (Bovine) 536558, (Human) 3308

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Function(s)
virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion glycolipid binding
Process(es)
response to unfolded protein male meiosis male meiosis I spermatogenesis spermatid development response to heat response to cold positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle protein refolding synaptonemal complex disassembly negative regulation of inclusion body assembly positive regulation of calcium-transporting ATPase activity positive regulation of gene expression regulation of cell death regulation of protein ubiquitination negative regulation of protein ubiquitination positive regulation of interleukin-8 production cellular response to oxidative stress cellular response to heat ATP metabolic process viral entry into host cell protein stabilization positive regulation of NF-kappaB transcription factor activity negative regulation of cell death cellular heat acclimation positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway regulation of cellular response to heat positive regulation of tumor necrosis factor-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand binding of sperm to zona pellucida positive regulation of protein targeting to mitochondrion cell differentiation telomere maintenance DNA repair regulation of mRNA stability cell-cell adhesion negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoribonuclease activity positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response protein import into mitochondrial outer membrane chaperone-mediated protein complex assembly positive regulation of microtubule nucleation regulation of mitotic spindle assembly
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