Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
HSP90 alpha Antibody (600-401-929) in WB
Western blot using Rocklands Affinity Purified anti-Hsp90 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt Hsp90 coupled to FlagTM tag. Anti-FlagTM immunoprecipitation... View More
Product Details
600-401-929
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 289-300 of human Hsp90 protein.
Conjugate
Form
Liquid
Concentration
1.24 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human Hsp90 protein. A BLAST analysis was used to suggest cross reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.
Target Information
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein HSP90 alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen
Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2
UniProt ID: (Human) P07900, (Chicken) P11501, (Mouse) P07901, (Rat) P82995
Entrez Gene ID: (Human) 3320, (Chicken) 423463, (Mouse) 15519, (Rat) 299331
nucleotide binding
UTP binding
CTP binding
mRNA binding
protein tyrosine kinase activity
protein binding
ATP binding
GTP binding
ATPase activity
sulfonylurea receptor binding
protein kinase binding
protein phosphatase binding
MHC class II protein complex binding
nitric-oxide synthase regulator activity
TPR domain binding
dATP binding
identical protein binding
protein homodimerization activity
histone deacetylase binding
ion channel binding
poly(A) RNA binding
GTPase binding
Rho GDP-dissociation inhibitor binding
unfolded protein binding
protein C-terminus binding
estrogen receptor binding
heat shock protein binding
progesterone receptor binding
virion binding
chaperone binding
Hsp90 protein binding
disordered domain specific binding
Hsp90 family chaperone
G2/M transition of mitotic cell cycle
neuron migration
skeletal muscle contraction
mitochondrial transport
receptor-mediated endocytosis
response to unfolded protein
signal transduction
response to heat
response to cold
response to salt stress
positive regulation of lamellipodium assembly
cardiac muscle cell apoptotic process
peptidyl-tyrosine phosphorylation
regulation of protein ubiquitination
positive regulation of protein import into nucleus, translocation
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB2 signaling pathway
protein refolding
regulation of protein complex assembly
protein unfolding
response to estrogen
protein import into mitochondrial outer membrane
positive regulation of nitric oxide biosynthetic process
positive regulation of cell size
response to antibiotic
vascular endothelial growth factor receptor signaling pathway
protein stabilization
regulation of nitric-oxide synthase activity
chaperone-mediated protein complex assembly
positive regulation of cardiac muscle contraction
chaperone-mediated autophagy
regulation of cellular response to heat
protein folding
positive regulation of cell proliferation
response to organonitrogen compound
response to organic cyclic compound
response to progesterone
cellular response to heat
regulation of catalytic activity
positive regulation of cell cycle G2/M phase transition
nitric oxide biosynthetic process
response to stress
positive regulation of cytotoxic T cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support