Invitrogen

HSP90 alpha Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

32 Published Figures

49 References

View all (31) HSP90 alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HSP90 alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 46

HSP90 alpha Antibody (PA3-013) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSP86 polyclonal antibody (Product # PA3-013), at a dilution of 1:100 for at least 1 hour at ro... View More

HSP90 alpha Antibody in Western Blot (WB)

HSP90 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP90 alpha Antibody in Immunoprecipitation (IP)

HSP90 alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA3-013

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

1:50-1:200

View 6 publications 6 publications

Immunoprecipitation (IP)

2 µg

View 3 publications 3 publications

ChIP assay (ChIP)

- -

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Sheep, Rat

Published species

Human, Mouse, Non-human primate, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2120934

Product Specific Information

PA3-013 detects heat shock protein 90 (HSP90) from human, monkey, mouse, sheep (adrenal) and rat tissues and cells. This antibody does not detect HSP84.

PA3-013 has been successfully used in Western blot, immunofluorescence, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Immunofluorescence staining of HSP86 in HeLa cells with PA3-013 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells. Furthermore, the antibody is capable of precipitating HSP86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

The PA3-013 immunogen is a synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.

Target Information

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bryant A,Li Z,Jayakumar R,Serrano-Pozo A,Woost B,Hu M,Woodbury ME,Wachter A,Lin G,Kwon T,Talanian RV,Biber K,Karran EH,Hyman BT,Das S,Bennett RE

PA3-013 was used in Immunohistochemistry to help explain why certain brain regions appear to differ in susceptibility to disease-related vascular remodeling events that may impact blood flow.

Wed Jun 14 00:00:00 EDT 2023

Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.

Cell death & disease

Kim MJ,Choi GE,Chae CW,Lim JR,Jung YH,Yoon JH,Park JY,Han HJ

PA3-013 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate how melatonin regulates chaperone proteins related to GR trafficking into the nucleus to suppress glucocorticoid action.

Tue Feb 21 00:00:00 EST 2023

Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.

Cell death & disease

Kim MJ,Choi GE,Chae CW,Lim JR,Jung YH,Yoon JH,Park JY,Han HJ

Tue Feb 21 00:00:00 EST 2023

Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.

Cell death & disease

Kim MJ,Choi GE,Chae CW,Lim JR,Jung YH,Yoon JH,Park JY,Han HJ

Tue Feb 21 00:00:00 EST 2023

Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.

Cell death & disease

Kim MJ,Choi GE,Chae CW,Lim JR,Jung YH,Yoon JH,Park JY,Han HJ

Tue Feb 21 00:00:00 EST 2023

Brain region-specific susceptibility of Lewy body pathology in synucleinopathies is governed by α-synuclein conformations.

Acta neuropathologica

de Boni L,Watson AH,Zaccagnini L,Wallis A,Zhelcheska K,Kim N,Sanderson J,Jiang H,Martin E,Cantlon A,Rovere M,Liu L,Sylvester M,Lashley T,Dettmer U,Jaunmuktane Z,Bartels T

PA3-013 was used in Western Blot to suggest that these disease-specific patterns of α-synuclein multimer destabilization in sporadic PD and DLB are caused by both regional neuronal vulnerability and "prion-like" aggregation transmission enabled by the destabilization of local endogenous α-synuclein protein.

Fri Apr 01 00:00:00 EDT 2022

HSP90 promotes radioresistance of cervical cancer cells via reducing FBXO6-mediated CD147 polyubiquitination.

Cancer science

Song Q,Wen J,Li W,Xue J,Zhang Y,Liu H,Han J,Ning T,Lu Z

PA3-013 was used in Western Blotting to show that HSP90 promotes radioresistance of cervical cancer cells partially via reducing FBXO6 mediated CD147 polyubiquitination.

Fri Apr 01 00:00:00 EDT 2022

Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism.

Cell reports

Garcia-Martin R,Brandao BB,Thomou T,Altindis E,Kahn CR

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Tue Jan 18 00:00:00 EST 2022

Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism.

Cell reports

Garcia-Martin R,Brandao BB,Thomou T,Altindis E,Kahn CR

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Tue Jan 18 00:00:00 EST 2022

A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communication.

PLoS genetics

Shackleford G,Marziali LN,Sasaki Y,Claessens A,Ferri C,Weinstock NI,Rossor AM,Silvestri NJ,Wilson ER,Hurley E,Kidd GJ,Manohar S,Ding D,Salvi RJ,Feltri ML,D'Antonio M,Wrabetz L

PA3-013 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that Schwann cells in P0T124M mutant mice cannot provide axons with sufficient trophic support, leading to reduced ATP biosynthesis and axonopathy.

Tue Nov 01 00:00:00 EDT 2022

Loss of ARID1A Promotes Epithelial-Mesenchymal Transition and Sensitizes Pancreatic Tumors to Proteotoxic Stress.

Cancer research

Tomihara H,Carbone F,Perelli L,Huang JK,Soeung M,Rose JL,Robinson FS,Lissanu Deribe Y,Feng N,Takeda M,Inoue A,Poggetto ED,Deem AK,Maitra A,Msaouel P,Tannir NM,Draetta GF,Viale A,Heffernan TP,Bristow CA,Carugo A,Genovese G

PA3-013 was used in Western Blot, Immunocytochemistry to demonstrate that loss of ARID1A promotes an epithelial-mesenchymal transition (EMT) phenotype and sensitizes PDAC cells to a clinical inhibitor of HSP90, NVP-AUY922, both in vitro and in vivo.

Fri Jan 15 00:00:00 EST 2021

Loss of ARID1A Promotes Epithelial-Mesenchymal Transition and Sensitizes Pancreatic Tumors to Proteotoxic Stress.

Cancer research

Fri Jan 15 00:00:00 EST 2021

Selective HSP90β inhibition results in TNF and TRAIL mediated HIF1α degradation.

Immunobiology

Heck AL,Mishra S,Prenzel T,Feulner L,Achhammer E,Särchen V,Blagg BSJ,Schneider-Brachert W,Schütze S,Fritsch J

PA3-013 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that selective inhibition of HSP90 isoforms together with death ligand stimulation may provide novel strategies for therapy of inflammatory diseases or cancer, in future.

Mon Mar 01 00:00:00 EST 2021

RINT1 Loss Impairs Retinogenesis Through TRP53-Mediated Apoptosis.

Frontiers in cell and developmental biology

Gomes AL,Matos-Rodrigues GE,Frappart PO,Martins RAP

PA3-013 was used in Immunocytochemistry to study the roles of RINT1 in CNS development by conditionally inactivating Rint1 in retinal progenitor cells (RPCs) during embryogenesis to bypass the lethality of Rint1 inactivation in the embryonic brain.

Mon Sep 28 00:00:00 EDT 2020

Adipose tissue-liver crosstalk during pathologic changes caused by vinyl chloride metabolites in mice.

Toxicology and applied pharmacology

Kaelin BR,McKenzie CM,Hempel KW,Lang AL,Arteel GE,Beier JI

PA3-013 was used in Western Blot to show that CE enhances local inflammation and alters lipid metabolism and WAT-mediated hepatic steatosis due to changes in WAT lipolysis.

Wed Jul 15 00:00:00 EDT 2020

HSP90A inhibition promotes anti-tumor immunity by reversing multi-modal resistance and stem-like property of immune-refractory tumors.

Nature communications

Song KH,Oh SJ,Kim S,Cho H,Lee HJ,Song JS,Chung JY,Cho E,Lee J,Jeon S,Yee C,Lee KM,Hewitt SM,Kim JH,Woo SR,Kim TW

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Tue Jan 28 00:00:00 EST 2020

Human coronavirus dependency on host heat shock protein 90 reveals an antiviral target.

Emerging microbes & infections

Li C,Chu H,Liu X,Chiu MC,Zhao X,Wang D,Wei Y,Hou Y,Shuai H,Cai J,Chan JF,Zhou J,Yuen KY

PA3-013 was used in Western Blotting to investigate the role of Hsp90 for coronavirus propagation.

Tue Dec 01 00:00:00 EST 2020

Heat Shock Protein 90 as a Prognostic Marker and Therapeutic Target for Adrenocortical Carcinoma.

Frontiers in endocrinology

Siebert C,Ciato D,Murakami M,Frei-Stuber L,Perez-Rivas LG,Monteserin-Garcia JL,Nölting S,Maurer J,Feuchtinger A,Walch AK,Haak HR,Bertherat J,Mannelli M,Fassnacht M,Korpershoek E,Reincke M,Stalla GK,Hantel C,Beuschlein F

PA3-013 was used in Western Blotting to examine the expression of HSP90 in different adrenal tumors and evaluate the use of HSP90 inhibitors in vitro as a possible therapy for adrenocortical carcinoma.

Tue Feb 25 00:00:00 EST 2020

Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant.

Nature communications

Cao Y,Zhu X,Hossen MN,Kakar P,Zhao Y,Chen X

PA3-013 was used in Western Blotting to study the safety and potency of non-invasive radiofrequency treatment to boost intradermal vaccination in murine models.

Wed Sep 12 00:00:00 EDT 2018

Molecular and functional crosstalk between extracellular Hsp90 and ephrin A1 signaling.

Oncotarget

Daoud A,Gopal U,Kaur J,Isaacs JS

PA3-013 was used in Western Blot to define the molecular and functional relationship between EphA2 and extracellular Hsp90.

Tue Dec 05 00:00:00 EST 2017

LSD1 engages a corepressor complex for the activation of the estrogen receptor α by estrogen and cAMP.

Nucleic acids research

Bennesch MA,Segala G,Wider D,Picard D

PA3-013 was used in western blot to assess activation of the estrogen receptor alpha by estrogen and cAMP and due to LSD1 engaging as a corepressor complex

Fri Oct 14 00:00:00 EDT 2016

Novobiocin Analogues That Inhibit the MAPK Pathway.

Journal of medicinal chemistry

Hall JA,Seedarala S,Zhao H,Garg G,Ghosh S,Blagg BS

PA3-013 was used in western blot to analyze inhibitors of the MAPK pathway that are novobiocin analogues

Thu Feb 11 00:00:00 EST 2016

A Remodeled Hsp90 Molecular Chaperone Ensemble with the Novel Cochaperone Aarsd1 Is Required for Muscle Differentiation.

Molecular and cellular biology

Echeverría PC,Briand PA,Picard D

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Fri Apr 01 00:00:00 EDT 2016

Acetylation of androgen receptor by ARD1 promotes dissociation from HSP90 complex and prostate tumorigenesis.

Oncotarget

DePaolo JS,Wang Z,Guo J,Zhang G,Qian C,Zhang H,Zabaleta J,Liu W

PA3013 was used in western blot to explore acetylation of arrest-defective protein 1 in prostate cancer cells

Tue Nov 01 00:00:00 EDT 2016

Acetylation of androgen receptor by ARD1 promotes dissociation from HSP90 complex and prostate tumorigenesis.

Oncotarget

DePaolo JS,Wang Z,Guo J,Zhang G,Qian C,Zhang H,Zabaleta J,Liu W

PA3013 was used in western blot to explore acetylation of arrest-defective protein 1 in prostate cancer cells

Tue Nov 01 00:00:00 EDT 2016

Acetylation of androgen receptor by ARD1 promotes dissociation from HSP90 complex and prostate tumorigenesis.

Oncotarget

DePaolo JS,Wang Z,Guo J,Zhang G,Qian C,Zhang H,Zabaleta J,Liu W

PA3013 was used in western blot to explore acetylation of arrest-defective protein 1 in prostate cancer cells

Tue Nov 01 00:00:00 EDT 2016

Acetylation of androgen receptor by ARD1 promotes dissociation from HSP90 complex and prostate tumorigenesis.

Oncotarget

DePaolo JS,Wang Z,Guo J,Zhang G,Qian C,Zhang H,Zabaleta J,Liu W

PA3013 was used in western blot to explore acetylation of arrest-defective protein 1 in prostate cancer cells

Tue Nov 01 00:00:00 EDT 2016

A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease.

Nature medicine

Riebold M,Kozany C,Freiburger L,Sattler M,Buchfelder M,Hausch F,Stalla GK,Paez-Pereda M

PA3-013 was used in western blot to investigate how HSP90 affects the glucocorticoid receptor in corticotroph cells.

Sun Mar 01 00:00:00 EST 2015

Integration-independent Transgenic Huntington Disease Fragment Mouse Models Reveal Distinct Phenotypes and Life Span in Vivo.

The Journal of biological chemistry

O'Brien R,DeGiacomo F,Holcomb J,Bonner A,Ring KL,Zhang N,Zafar K,Weiss A,Lager B,Schilling B,Gibson BW,Chen S,Kwak S,Ellerby LM

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Fri Jul 31 00:00:00 EDT 2015

Integration-independent Transgenic Huntington Disease Fragment Mouse Models Reveal Distinct Phenotypes and Life Span in Vivo.

The Journal of biological chemistry

O'Brien R,DeGiacomo F,Holcomb J,Bonner A,Ring KL,Zhang N,Zafar K,Weiss A,Lager B,Schilling B,Gibson BW,Chen S,Kwak S,Ellerby LM

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Fri Jul 31 00:00:00 EDT 2015

HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse.

Nucleic acids research

Ichiyanagi T,Ichiyanagi K,Ogawa A,Kuramochi-Miyagawa S,Nakano T,Chuma S,Sasaki H,Udono H

PA3-013 was used in Immunohistochemistry-immunofluorescence to study the specialised function of the HSP90α isoform in PIWI-interacting RNA biogenesis and repression of retrotransposons during the development of male germ cells in mammals.

Wed Oct 29 00:00:00 EDT 2014

Cruentaren A binds F1F0 ATP synthase to modulate the Hsp90 protein folding machinery.

ACS chemical biology

Hall JA,Kusuma BR,Brandt GE,Blagg BS

PA3-013 was used in western blot to study the lack of induction of the pro-survival heat shock response following disruption of the interaction between F1F0 ATP synthase and Hsp90 by cruentaren A

Fri Apr 18 00:00:00 EDT 2014

c-Myc regulates cell proliferation during lens development.

PloS one

Cavalheiro GR,Matos-Rodrigues GE,Gomes AL,Rodrigues PM,Martins RA

PA3-013 was used in Immunohistochemistry to support the conclusion that c-myc regulates the proliferation of lens epithelial cells in vivo and modulate the expression of classical cell cycle regulators in developing mouse lens.

Mon Dec 22 00:00:00 EST 2014

Vacuolar H+ ATPase expression and activity is required for Rab27B-dependent invasive growth and metastasis of breast cancer.

International journal of cancer

Hendrix A,Sormunen R,Westbroek W,Lambein K,Denys H,Sys G,Braems G,Van den Broecke R,Cocquyt V,Gespach C,Bracke M,De Wever O

PA3-013 was used in western blot to study the mechanism by which vacuolar H(+)-ATPase regulates Rab27B-mediated invasion and metastases in estrogen receptor-positive breast cancer

Thu Aug 15 00:00:00 EDT 2013

Selective inhibition of HER2-positive breast cancer cells by the HIV protease inhibitor nelfinavir.

Journal of the National Cancer Institute

Shim JS,Rao R,Beebe K,Neckers L,Han I,Nahta R,Liu JO

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Wed Oct 17 00:00:00 EDT 2012

A systematic protocol for the characterization of Hsp90 modulators.

Bioorganic & medicinal chemistry

Matts RL,Brandt GE,Lu Y,Dixit A,Mollapour M,Wang S,Donnelly AC,Neckers L,Verkhivker G,Blagg BS

PA3-013 was used in western blot to devise a method for the investigation of Hsp90 modulators

Sat Jan 01 00:00:00 EST 2011

A systematic protocol for the characterization of Hsp90 modulators.

Bioorganic & medicinal chemistry

Matts RL,Brandt GE,Lu Y,Dixit A,Mollapour M,Wang S,Donnelly AC,Neckers L,Verkhivker G,Blagg BS

PA3-013 was used in western blot to devise a method for the investigation of Hsp90 modulators

Sat Jan 01 00:00:00 EST 2011

An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine.

PloS one

Echeverría PC,Bernthaler A,Dupuis P,Mayer B,Picard D

PA3-013 was used in immunohistochemistry and immunoprecipitation to evaluate an interaction network for the prediction of protein functions

Fri Feb 17 00:00:00 EST 2012

Transactivation of the epidermal growth factor receptor by heat shock protein 90 via Toll-like receptor 4 contributes to the migration of glioblastoma cells.

The Journal of biological chemistry

Thuringer D,Hammann A,Benikhlef N,Fourmaux E,Bouchot A,Wettstein G,Solary E,Garrido C

PA3-013 was used in immunocytochemistry to investigate the role of heat shock protein 90 in glioblastoma cell migration

Fri Feb 04 00:00:00 EST 2011

Subcellular heterogeneity of ryanodine receptor properties in ventricular myocytes with low T-tubule density.

PloS one

Biesmans L,Macquaide N,Heinzel FR,Bito V,Smith GL,Sipido KR

PA3-013 was used in immunocytochemistry to characterize the ryanodine receptor in ventricular myocytes

Fri Feb 17 00:00:00 EST 2012

Effect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis.

Journal of the National Cancer Institute

Hendrix A,Maynard D,Pauwels P,Braems G,Denys H,Van den Broecke R,Lambert J,Van Belle S,Cocquyt V,Gespach C,Bracke M,Seabra MC,Gahl WA,De Wever O,Westbroek W

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Wed Jun 16 00:00:00 EDT 2010

The molecular chaperone Hsp90α is required for meiotic progression of spermatocytes beyond pachytene in the mouse.

PloS one

Grad I,Cederroth CR,Walicki J,Grey C,Barluenga S,Winssinger N,De Massy B,Nef S,Picard D

Published figure using HSP90 alpha polyclonal antibody (Product # PA3-013) in Western Blot

Fri Dec 31 00:00:00 EST 2010

KU135, a novel novobiocin-derived C-terminal inhibitor of the 90-kDa heat shock protein, exerts potent antiproliferative effects in human leukemic cells.

Molecular pharmacology

Shelton SN,Shawgo ME,Matthews SB,Lu Y,Donnelly AC,Szabla K,Tanol M,Vielhauer GA,Rajewski RA,Matts RL,Blagg BS,Robertson JD

PA3-013 was used in western blot to investigate the effect of a novel novobiocin-derived C-terminal Hsp90 inhibitor,KU135,on human leukemic cells

Tue Dec 01 00:00:00 EST 2009

(-)-Epigallocatechin-3-gallate is a novel Hsp90 inhibitor.

Biochemistry

Yin Z,Henry EC,Gasiewicz TA

PA3-013 was used in immunoprecipitation and western blot to investigate the inhibitory effect of (-)-epigallocatechin-3-gallate on Hsp90

Tue Jan 20 00:00:00 EST 2009

(-)-Epigallocatechin-3-gallate is a novel Hsp90 inhibitor.

Biochemistry

Yin Z,Henry EC,Gasiewicz TA

PA3-013 was used in immunoprecipitation and western blot to investigate the inhibitory effect of (-)-epigallocatechin-3-gallate on Hsp90

Tue Jan 20 00:00:00 EST 2009

Pregnancy-enhanced endothelial nitric oxide synthase (eNOS) activation in uterine artery endothelial cells shows altered sensitivity to Ca2+, U0126, and wortmannin but not LY294002--evidence that pregnancy adaptation of eNOS activation occurs at multiple levels of cell signaling.

Endocrinology

Sullivan JA,Grummer MA,Yi FX,Bird IM

PA3-013 was used in western blot to investigate the roles of calcium and multiple kinases in the regulation of endothelial NO synthase activity.

Mon May 01 00:00:00 EDT 2006

Domain-mediated dimerization of the Hsp90 cochaperones Harc and Cdc37.

Biochemistry

Roiniotis J,Masendycz P,Ho S,Scholz GM

PA3-013 was used in western blot to investigate the role of heat Shock Protein 90 cochaperones Harc and Cdc37 in protein folding and its mechanism

Tue May 03 00:00:00 EDT 2005

Functional characterization of HUVEC-CS: Ca2+ signaling, ERK 1/2 activation, mitogenesis and vasodilator production.

The Journal of endocrinology

Gifford SM,Grummer MA,Pierre SA,Austin JL,Zheng J,Bird IM

PA3-013 was used in western blot to study Ca2+ signaling, ERK1/2 activation, mitogenesis and vasodilator production in an immortalized HUVEC subline

Wed Sep 01 00:00:00 EDT 2004

Purification of polyglutamine aggregates and identification of elongation factor-1alpha and heat shock protein 84 as aggregate-interacting proteins.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mitsui K,Nakayama H,Akagi T,Nekooki M,Ohtawa K,Takio K,Hashikawa T,Nukina N

PA3-013 was used in western blot to investigate the roles of the EF-1alpha and HSP84 in the neurodegenerative process of polyglutamine diseases.

Fri Nov 01 00:00:00 EST 2002

Characterization of the phosphorylation status of the hepatitis B virus X-associated protein 2.

Archives of biochemistry and biophysics

Dull AB,Carlson DB,Petrulis JR,Perdew GH

PA3-013 was used in western blot to indentify the phosphorylation status of the hepatitis B virus X-associated protein 2

Tue Oct 15 00:00:00 EDT 2002

p50(cdc37) is a nonexclusive Hsp90 cohort which participates intimately in Hsp90-mediated folding of immature kinase molecules.

Biochemistry

Hartson SD,Irwin AD,Shao J,Scroggins BT,Volk L,Huang W,Matts RL

PA3-013 was used in western blot to investigate the role of p50(cdc37) in heat shock protein 90 molecular chaperone function

Tue Jun 27 00:00:00 EDT 2000

Hsp and chaperone distribution during endochondral bone development in mouse embryo.

Cell stress & chaperones

Loones MT,Morange M

PA3-013 was used in immunohistochemistry to study the expression of heat shock proteins and chaperones during endochondral bone development in mouse

Tue Dec 01 00:00:00 EST 1998

Modular folding and evidence for phosphorylation-induced stabilization of an hsp90-dependent kinase.

The Journal of biological chemistry

Hartson SD,Ottinger EA,Huang W,Barany G,Burn P,Matts RL

PA3-013 was used in western blot to characterize a hsp90-dependent kinase and its regulation by phosphorylation

Fri Apr 03 00:00:00 EST 1998

Function and regulation of heat shock factor 2 during mouse embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Rallu M,Loones M,Lallemand Y,Morimoto R,Morange M,Mezger V

PA3-013 was used in immunocytochemistry to characterize heat shock factor 2 during mouse embryogenesis

Tue Mar 18 00:00:00 EST 1997

Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex.

The Journal of biological chemistry

Chen HS,Perdew GH

PA3-013 was used in western blot to identify the subunits of AhR complex

Fri Nov 04 00:00:00 EST 1994

Bioinformatics

Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein alpha; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen

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Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2

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UniProt ID: (Human) P07900, (Mouse) P07901, (Rat) P82995

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Entrez Gene ID: (Human) 3320, (Sheep) 100127209, (Mouse) 15519, (Rat) 299331

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Function(s)

nucleotide binding UTP binding CTP binding mRNA binding protein tyrosine kinase activity protein binding ATP binding GTP binding ATPase activity sulfonylurea receptor binding protein kinase binding protein phosphatase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding identical protein binding protein homodimerization activity histone deacetylase binding ion channel binding poly(A) RNA binding GTPase binding Rho GDP-dissociation inhibitor binding unfolded protein binding Hsp90 family chaperone

Process(es)

G2/M transition of mitotic cell cycle neuron migration skeletal muscle contraction mitochondrial transport receptor-mediated endocytosis response to unfolded protein signal transduction response to heat response to cold response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process peptidyl-tyrosine phosphorylation regulation of protein ubiquitination positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway protein refolding regulation of protein complex assembly protein unfolding response to estrogen protein import into mitochondrial outer membrane positive regulation of nitric oxide biosynthetic process positive regulation of cell size response to antibiotic vascular endothelial growth factor receptor signaling pathway protein stabilization regulation of nitric-oxide synthase activity chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy regulation of cellular response to heat protein folding nitric oxide biosynthetic process response to stress positive regulation of cytotoxic T cell differentiation regulation of catalytic activity

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HSP90 alpha Polyclonal Antibody

HSP90 alpha Antibody (PA3-013) in ICC/IF

Product Details

PA3-013

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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