Invitrogen

HSP90 alpha Monoclonal Antibody (Hyb-K41009), FITC

View all (31) HSP90 alpha antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45105

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:5,000
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

Hyb-K41009

Immunogen

Recombinant human HSP90alpha; Specificity mapped to amino acids 604-731
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.2, with 581mM DMSO, 123mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931559

Product Specific Information

1 µg/mL of MA5-45105 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects 90kDa. This is an alpha-specific product.

Target Information

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen

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Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2

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UniProt ID: (Human) P07900, (Mouse) P07901, (Rat) P82995

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Entrez Gene ID: (Human) 3320, (Mouse) 15519, (Rat) 299331

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Function(s)
nucleotide binding UTP binding CTP binding mRNA binding protein tyrosine kinase activity protein binding ATP binding GTP binding ATPase activity sulfonylurea receptor binding protein kinase binding protein phosphatase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding identical protein binding protein homodimerization activity histone deacetylase binding ion channel binding poly(A) RNA binding GTPase binding Rho GDP-dissociation inhibitor binding unfolded protein binding Hsp90 family chaperone
Process(es)
G2/M transition of mitotic cell cycle neuron migration skeletal muscle contraction mitochondrial transport receptor-mediated endocytosis response to unfolded protein signal transduction response to heat response to cold response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process peptidyl-tyrosine phosphorylation regulation of protein ubiquitination positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway protein refolding regulation of protein complex assembly protein unfolding response to estrogen protein import into mitochondrial outer membrane positive regulation of nitric oxide biosynthetic process positive regulation of cell size response to antibiotic vascular endothelial growth factor receptor signaling pathway protein stabilization regulation of nitric-oxide synthase activity chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy regulation of cellular response to heat protein folding nitric oxide biosynthetic process response to stress positive regulation of cytotoxic T cell differentiation regulation of catalytic activity
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