Invitrogen

HSP90 beta Polyclonal Antibody

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

17 Published Figures

28 References

View all (39) HSP90 beta antibodies

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Questions & Answers

Datasheet

Protocols

Questions & Answers

HSP90 beta Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 29

HSP90 beta Antibody (PA3-012) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 84 using Anti-Heat Shock Protein 84 Polyclonal Antibody (Product # PA3-012) shows staining in U251 Cells. Heat Shock Protein 84 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibo... View More

HSP90 beta Antibody in Western Blot (WB)

HSP90 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP90 beta Antibody in Immunoprecipitation (IP)

HSP90 beta Antibody in Flow Cytometry (Flow)

HSP90 beta Antibody in Immunohistochemistry (IHC)

HSP90 beta Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA3-012

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:100

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

10-20 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

1-20 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2121220

Product Specific Information

PA3-012 detects heat shock protein 90 beta (HSP90) from human, monkey, mouse, and rat tissues and cells. This antibody does not detect HSP86.

PA3-012 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~84 kDa protein representing HSP84 from Hepa1 cell lysate. Immunofluorescence staining of HSP84 in Hepa1 cells with PA3-012 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP84 exists primarily as homodimers in Hepa 1 cells. Furthermore, the antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

The PA3-012 immunogen is a synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NF-κB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells.

Nature immunology

Zhao M,Chauhan P,Sherman CA,Singh A,Kaileh M,Mazan-Mamczarz K,Ji H,Joy J,Nandi S,De S,Zhang Y,Fan J,Becker KG,Loke P,Zhou W,Sen R

PA3-012 was used in Flow cytometry/Cell sorting to provide insights into exclusive functions of Rel and RelA in immunity and cancer.

Fri Sep 01 00:00:00 EDT 2023

HSP90 promotes radioresistance of cervical cancer cells via reducing FBXO6-mediated CD147 polyubiquitination.

Cancer science

Song Q,Wen J,Li W,Xue J,Zhang Y,Liu H,Han J,Ning T,Lu Z

PA3-012 was used in Western Blotting to show that HSP90 promotes radioresistance of cervical cancer cells partially via reducing FBXO6 mediated CD147 polyubiquitination.

Fri Apr 01 00:00:00 EDT 2022

Selective HSP90β inhibition results in TNF and TRAIL mediated HIF1α degradation.

Immunobiology

Heck AL,Mishra S,Prenzel T,Feulner L,Achhammer E,Särchen V,Blagg BSJ,Schneider-Brachert W,Schütze S,Fritsch J

PA3-012 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to suggest that selective inhibition of HSP90 isoforms together with death ligand stimulation may provide novel strategies for therapy of inflammatory diseases or cancer, in future.

Mon Mar 01 00:00:00 EST 2021

Human coronavirus dependency on host heat shock protein 90 reveals an antiviral target.

Emerging microbes & infections

Li C,Chu H,Liu X,Chiu MC,Zhao X,Wang D,Wei Y,Hou Y,Shuai H,Cai J,Chan JF,Zhou J,Yuen KY

PA3-012 was used in Immunoprecipitation to investigate the role of Hsp90 for coronavirus propagation.

Tue Dec 01 00:00:00 EST 2020

Human coronavirus dependency on host heat shock protein 90 reveals an antiviral target.

Emerging microbes & infections

Li C,Chu H,Liu X,Chiu MC,Zhao X,Wang D,Wei Y,Hou Y,Shuai H,Cai J,Chan JF,Zhou J,Yuen KY

PA3-012 was used in Immunoprecipitation to investigate the role of Hsp90 for coronavirus propagation.

Tue Dec 01 00:00:00 EST 2020

Human coronavirus dependency on host heat shock protein 90 reveals an antiviral target.

Emerging microbes & infections

Li C,Chu H,Liu X,Chiu MC,Zhao X,Wang D,Wei Y,Hou Y,Shuai H,Cai J,Chan JF,Zhou J,Yuen KY

PA3-012 was used in Immunoprecipitation to investigate the role of Hsp90 for coronavirus propagation.

Tue Dec 01 00:00:00 EST 2020

Heat Shock Protein 90 as a Prognostic Marker and Therapeutic Target for Adrenocortical Carcinoma.

Frontiers in endocrinology

Siebert C,Ciato D,Murakami M,Frei-Stuber L,Perez-Rivas LG,Monteserin-Garcia JL,Nölting S,Maurer J,Feuchtinger A,Walch AK,Haak HR,Bertherat J,Mannelli M,Fassnacht M,Korpershoek E,Reincke M,Stalla GK,Hantel C,Beuschlein F

PA3-012 was used in Western Blotting to examine the expression of HSP90 in different adrenal tumors and evaluate the use of HSP90 inhibitors in vitro as a possible therapy for adrenocortical carcinoma.

Tue Feb 25 00:00:00 EST 2020

Heat Shock Protein 90 as a Prognostic Marker and Therapeutic Target for Adrenocortical Carcinoma.

Frontiers in endocrinology

Tue Feb 25 00:00:00 EST 2020

HIV-1 restriction by endogenous APOBEC3G in the myeloid cell line THP-1.

The Journal of general virology

Ikeda T,Molan AM,Jarvis MC,Carpenter MA,Salamango DJ,Brown WL,Harris RS

PA3-012 was used in Western Blotting to study whether the rules for HIV-1 restriction by A3 enzymes are similar in myeloid and T cell lines.

Mon Jul 01 00:00:00 EDT 2019

HIV-1 restriction by endogenous APOBEC3G in the myeloid cell line THP-1.

The Journal of general virology

Ikeda T,Molan AM,Jarvis MC,Carpenter MA,Salamango DJ,Brown WL,Harris RS

PA3-012 was used in Western Blotting to study whether the rules for HIV-1 restriction by A3 enzymes are similar in myeloid and T cell lines.

Mon Jul 01 00:00:00 EDT 2019

APOBEC3B lysine residues are dispensable for DNA cytosine deamination, HIV-1 restriction, and nuclear localization.

Virology

Molan AM,Hanson HM,Chweya CM,Anderson BD,Starrett GJ,Richards CM,Harris RS

PA3-012 was used in Western Blotting to examine the role of APOBEC3B lysine residues in DNA cytosine deamination, HIV-1 restriction, and nuclear localisation.

Wed Nov 01 00:00:00 EDT 2017

LSD1 engages a corepressor complex for the activation of the estrogen receptor α by estrogen and cAMP.

Nucleic acids research

Bennesch MA,Segala G,Wider D,Picard D

PA3-012 was used in Western Blot to identify the histone H3 demethylase, LSD1, as a substrate of protein kinase A and an important mediator of signalling crosstalk and the response to oestrogen.

Fri Oct 14 00:00:00 EDT 2016

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling.

Journal of extracellular vesicles

Van Deun J,Mestdagh P,Sormunen R,Cocquyt V,Vermaelen K,Vandesompele J,Bracke M,De Wever O,Hendrix A

Published figure using HSP90 beta polyclonal antibody (Product # PA3-012) in Western Blot

Wed Oct 15 00:00:00 EDT 2014

Vacuolar H+ ATPase expression and activity is required for Rab27B-dependent invasive growth and metastasis of breast cancer.

International journal of cancer

Hendrix A,Sormunen R,Westbroek W,Lambein K,Denys H,Sys G,Braems G,Van den Broecke R,Cocquyt V,Gespach C,Bracke M,De Wever O

PA3-012 was used in western blot to study the mechanism by which vacuolar H(+)-ATPase regulates Rab27B-mediated invasion and metastases in estrogen receptor-positive breast cancer

Thu Aug 15 00:00:00 EDT 2013

Heat shock protein 90α (HSP90α), a substrate and chaperone of DNA-PK necessary for the apoptotic response.

Proceedings of the National Academy of Sciences of the United States of America

Solier S,Kohn KW,Scroggins B,Xu W,Trepel J,Neckers L,Pommier Y

PA3-012 was used in western blot to study the role of Hsp90 as both a substrate and chaperone of DNA-dependent protein kinase in the apoptotic response

Tue Aug 07 00:00:00 EDT 2012

Effect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis.

Journal of the National Cancer Institute

Hendrix A,Maynard D,Pauwels P,Braems G,Denys H,Van den Broecke R,Lambert J,Van Belle S,Cocquyt V,Gespach C,Bracke M,Seabra MC,Gahl WA,De Wever O,Westbroek W

PA3-012 was used in western blot to investigate the role of secretory small GTPase Rab27B in breast cancer proliferation and metastasis

Wed Jun 16 00:00:00 EDT 2010

Mutations that increase both Hsp90 ATPase activity in vitro and Hsp90 drug resistance in vivo.

Biochimica et biophysica acta

Zurawska A,Urbanski J,Matuliene J,Baraniak J,Klejman MP,Filipek S,Matulis D,Bieganowski P

PA3-012 was used in western blot to investigate the consequences of point mutations in heat shock protein 90 gene

Sat May 01 00:00:00 EDT 2010

Proteomic identification of differentially expressed genes in mouse neural stem cells and neurons differentiated from embryonic stem cells in vitro.

Biochimica et biophysica acta

Akama K,Tatsuno R,Otsu M,Horikoshi T,Nakayama T,Nakamura M,Toda T,Inoue N

PA3-012 was used in western blot to identify the differentially expressed proteins in neural stem cells and embryonic stem cells

Thu May 01 00:00:00 EDT 2008

Changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Papp E,Száraz P,Korcsmáros T,Csermely P

PA3-012 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice

Mon May 01 00:00:00 EDT 2006

Functional and cellular responses in a novel rodent model of anterior ischemic optic neuropathy.

Investigative ophthalmology & visual science

Bernstein SL,Guo Y,Kelman SE,Flower RW,Johnson MA

PA3-012 was used in immunohistochemistry to evaluate a novel rodent anterior ischemic optic neuropathy model

Wed Oct 01 00:00:00 EDT 2003

Comparative analysis of the ATP-binding sites of Hsp90 by nucleotide affinity cleavage: a distinct nucleotide specificity of the C-terminal ATP-binding site.

European journal of biochemistry

Soti C,Vermes A,Haystead TA,Csermely P

PA3-012 was used in western blot to study the specificity of the N-terminal and C-terminal Hsp90 nucleotide binding sites

Sun Jun 01 00:00:00 EDT 2003

Purification of polyglutamine aggregates and identification of elongation factor-1alpha and heat shock protein 84 as aggregate-interacting proteins.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mitsui K,Nakayama H,Akagi T,Nekooki M,Ohtawa K,Takio K,Hashikawa T,Nukina N

Published figure using HSP90 beta polyclonal antibody (Product # PA3-012) in Western Blot

Fri Nov 01 00:00:00 EST 2002

A Nucleotide-dependent molecular switch controls ATP binding at the C-terminal domain of Hsp90. N-terminal nucleotide binding unmasks a C-terminal binding pocket.

The Journal of biological chemistry

Söti C,Rácz A,Csermely P

Published figure using HSP90 beta polyclonal antibody (Product # PA3-012) in Western Blot

Fri Mar 01 00:00:00 EST 2002

Overexpression of murine small heat shock protein HSP25 interferes with chondrocyte differentiation and decreases cell adhesion.

Cell death and differentiation

Favet N,Duverger O,Loones MT,Poliard A,Kellermann O,Morange M

PA3-012 was used in Immunocytochemistry to suggest that overexpressing HSP25 decreases cellular adhesion and interferes with chondrocyte differentiation.

Fri Jun 01 00:00:00 EDT 2001

Hsp and chaperone distribution during endochondral bone development in mouse embryo.

Cell stress & chaperones

Loones MT,Morange M

PA3-012 was used in immunohistochemistry to study the expression of heat shock proteins and chaperones during endochondral bone development in mouse

Tue Dec 01 00:00:00 EST 1998

Function and regulation of heat shock factor 2 during mouse embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Rallu M,Loones M,Lallemand Y,Morimoto R,Morange M,Mezger V

PA3-012 was used in immunocytochemistry to characterize heat shock factor 2 during mouse embryogenesis

Tue Mar 18 00:00:00 EST 1997

Heat shock protein 84 forms a complex with mutant p53 protein predominantly within a cytoplasmic compartment of the cell.

The Journal of biological chemistry

Sepehrnia B,Paz IB,Dasgupta G,Momand J

Published figure using HSP90 beta polyclonal antibody (Product # PA3-012) in Immunoprecipitation

Fri Jun 21 00:00:00 EDT 1996

Heat shock protein 84 forms a complex with mutant p53 protein predominantly within a cytoplasmic compartment of the cell.

The Journal of biological chemistry

Sepehrnia B,Paz IB,Dasgupta G,Momand J

Published figure using HSP90 beta polyclonal antibody (Product # PA3-012) in Immunoprecipitation

Fri Jun 21 00:00:00 EDT 1996

Heat shock protein 84 forms a complex with mutant p53 protein predominantly within a cytoplasmic compartment of the cell.

The Journal of biological chemistry

Sepehrnia B,Paz IB,Dasgupta G,Momand J

Published figure using HSP90 beta polyclonal antibody (Product # PA3-012) in Immunoprecipitation

Fri Jun 21 00:00:00 EDT 1996

Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex.

The Journal of biological chemistry

Chen HS,Perdew GH

PA3-012 was used in western blot to identify the subunits of AhR complex

Fri Nov 04 00:00:00 EST 1994

Bioinformatics

Protein Aliases: Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein family C member 3; Heat shock protein HSP 90-beta; heat shock protein, 84 kDa 1; HSP; HSP 84; HSP 90; HSP84; HSP90-beta; retinal degeneration slow protein; RP1-302G2.1; TSTA; Tumor-specific transplantation 84 kDa antigen

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Gene Aliases: 90kDa; AL022974; C81438; D6S182; HSP84; Hsp84-1; Hsp90; HSP90-BETA; HSP90AB1; HSP90B; HSPC2; HSPC3; HSPCB

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UniProt ID: (Human) P08238, (Mouse) P11499, (Rat) P34058

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Entrez Gene ID: (Human) 3326, (Mouse) 15516, (Rat) 301252

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Function(s)

UTP binding CTP binding double-stranded RNA binding protein binding ATP binding GTP binding sulfonylurea receptor binding protein kinase regulator activity kinase binding protein kinase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding histone deacetylase binding ion channel binding poly(A) RNA binding unfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding Hsp90 family chaperone

Process(es)

placenta development protein folding response to unfolded protein response to salt stress virion attachment to host cell regulation of protein ubiquitination positive regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of phosphoprotein phosphatase activity positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of cell size protein stabilization regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway cellular response to interleukin-4 cellular response to organic cyclic compound positive regulation of protein serine/threonine kinase activity fibril organization cell-cell adhesion regulation of cellular response to heat response to stress

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HSP90 beta Polyclonal Antibody

HSP90 beta Antibody (PA3-012) in ICC/IF

Product Details

PA3-012

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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