Invitrogen

HSPA1A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

4 Published Figures
4 References
View all (34) HSPA1A antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-34772

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:20,000
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment contains a sequence corresponding to a region within amino acids 308 and 569 of HSP70 1A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552124

Product Specific Information

Recommended positive controls: 293T, NIH-3T3, Mouse brain, PC-12, Rat brain, Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, Mesenchymal stem cells.

Predicted reactivity: Mouse (97%), Rat (98%), Xenopus laevis (85%), Sheep (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock protein 70-1; Heat shock protein family A member 1A; Heat shock protein family A member 1B; heat shock protein, 70 kDa 3; heat shock-induced protein; Hsp68; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; inducible heat shock protein 70

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Gene Aliases: HEL-S-103; hsp68; HSP70-1; HSP70-1A; Hsp70-2; Hsp70-3; HSP70.1; Hsp70.3; Hsp70A1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; Hspa2; HSX70

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UniProt ID: (Human) P0DMV8, (Mouse) Q61696, (Rat) P0DMW0

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Entrez Gene ID: (Human) 3303, (Mouse) 193740, (Rat) 24472

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Function(s)
virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATP binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion protease binding double-stranded RNA binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding
Process(es)
telomere maintenance DNA repair protein folding response to heat positive regulation of gene expression regulation of cell death regulation of protein ubiquitination negative regulation of protein ubiquitination positive regulation of interleukin-8 production cellular response to oxidative stress cellular response to heat protein refolding regulation of mRNA stability ATP metabolic process viral entry into host cell protein stabilization positive regulation of NF-kappaB transcription factor activity negative regulation of cell death cellular heat acclimation positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway negative regulation of inclusion body assembly cell-cell adhesion regulation of cellular response to heat negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoribonuclease activity positive regulation of tumor necrosis factor-mediated signaling pathway positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response negative regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of T cell mediated cytotoxicity mRNA catabolic process defense response response to unfolded protein binding of sperm to zona pellucida negative regulation of cell proliferation negative regulation of cell growth negative regulation of myeloid cell apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of erythrocyte differentiation negative regulation of vasoconstriction
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