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Invitrogen
HSPA4 Monoclonal Antibody (3A11)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
HSPA4 Antibody (MA5-19153) in WB
Western blot was performed using Anti-HSPA4 Mouse Monoclonal Antibody (3A11) (Product # MA5-19153) and a 110kDa band corresponding to HSPA4 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3), A549 (Lane 4), SK-BR-3 (Lane 5), NIH/3T3 (Lane 6), PC-12 (Lane 7), COS-7 (Lane 8), MDCK (Lane 9), Mouse Testis (Lane 10) and Rat Ovary (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transfer... View More
Product Details
MA5-19153
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
3A11
Immunogen
HSPA4 (AAH02526, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608933
Product Specific Information
Peptide Sequence: MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEEQ NGPVDGQGDN PGPQAAEQGT DTAVPSDSDK KLPEMDID
Target Information
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock protein; Heat shock protein family H member 2; heat shock protein, 110 kDa; hsp70 RY; HSP70RY
Gene Aliases: APG-2; APG2; HEL-S-5a; HS24/P52; hsp70; hsp70RY; HSPA4; HSPH2; RY
UniProt ID: (Human) P34932
Entrez Gene ID: (Human) 3308
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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