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Heat Shock Factor 1 Antibody (500-7654) in IHC (P)
Immunohistochemistry: Human breast carcinoma tissue (FFPE) stained with Rat anti HSF1 antibody (Cat# 500-7654) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Product Details
500-7654
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ABM401
Immunogen
A recombinant mouse HSF1 (1-503aa) protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Positive control: LS174T cells. Breast carcinoma.
Cellular location: Nuclear.
Target Information
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1
Gene Aliases: AA960185; HSF1; Hsf1alpha; Hsf1beta; HSTF1
UniProt ID: (Human) Q00613, (Mouse) P38532
Entrez Gene ID: (Human) 3297, (Rat) 79245, (Mouse) 15499
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
embryonic placenta development
protein phosphorylation
defense response
female meiotic division
spermatogenesis
negative regulation of cell proliferation
mRNA transcription
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
cellular response to heat
positive regulation of multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
embryonic process involved in female pregnancy
regulation of cellular response to heat
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
response to heat
transcription from RNA polymerase II promoter
regulation of spindle checkpoint
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