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Hemoglobin A (beta chain) Antibody (200-301-GS4) in WB
Western Blot of Mouse Anti-Hemoglobin A (beta chain) Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50ng per lane. Primary antibody: Anti-HbA antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit ... View More
Product Details
200-301-GS4
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, lambda
Class
Monoclonal
Type
Antibody
Clone
14G2.G11.F11
Immunogen
Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Reacts specifically with human HbA beta chain isoform. This antibody does not react with the HbS form, HbF (gamma) form, HbC forms. HbA antibody cross reacts with HbA-2.
Target Information
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon-gamma-G-gamma-A-delta-beta-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta globin chain; Beta-globin; hemoglobin beta; Hemoglobin beta chain; Hemoglobin subunit beta; hemoglobin, beta
Gene Aliases: beta-globin; CD113t-C; HBB
UniProt ID: (Human) P68871
Entrez Gene ID: (Human) 3043
receptor-mediated endocytosis
blood coagulation
regulation of blood pressure
positive regulation of cell death
oxygen transport
bicarbonate transport
nitric oxide transport
response to hydrogen peroxide
hydrogen peroxide catabolic process
positive regulation of nitric oxide biosynthetic process
regulation of blood vessel size
protein heterooligomerization
renal absorption
platelet aggregation
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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