Invitrogen

Hepatitis B Virus X Monoclonal Antibody (X36C)

Hepatitis B Virus X Antibody in Western Blot (WB)

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Hepatitis B Virus X Antibody (MA1-081) in WB

Oncology letters 2021 - Figure 1. HBx increases NAMPT expression in liver cancer cells. (A) RT-qPCR and western blot analyses of NAMPT expression were performed in (A) HepG2 and HepG2.2.15 cells and (B) HepAD38 cells with or without Tet treatment. RT-qPCR and western blot analyses of NAMPT expression in (C) HepG2 and (D) Huh7 cells transfected with HBV1.3 or HBx plasmid. RT-qPCR and western blot analysis of NAMPT expression in (E)... View More

Hepatitis B Virus X Antibody in ChIP Assay (ChIP)

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Product Details

MA1-081

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

X36C

Immunogen

Baculovirus expressed recombinant HBx.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

HPLC

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325418

Product Specific Information

MA1-081 detects hepatitis B x (HBx) protein from transfected human cells.

MA1-081 has been successfully used in Western blot, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects a single ~17 kDa protein representing HBx. This antibody is not capable of immunoprecipitating HBx complexed with HBx interacting protein (XIP).

The MA1-081 immunogen is baculovirus expressed recombinant HBx.

Target Information

The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The Hepatitis B X antigen is a 154 amino acid ~17 kDa multifunctional protein involved in the development of liver chirrosis and hepatocellular carcinoma. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries. Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NAMPT promotes hepatitis B virus replication and liver cancer cell proliferation through the regulation of aerobic glycolysis.

Oncology letters

Guo HJ,Li HY,Chen ZH,Zhou WJ,Li JJ,Zhang JY,Wang J,Luo XY,Zeng T,Shi Z,Mo CF

MA1-081 was used in Western Blot to highlight the important role of NAMPT in the regulation of aerobic glycolysis in HBV-mediated liver cancer.

Sat May 01 00:00:00 EDT 2021

Cordyceps sinensis attenuates HBx‑induced cell apoptosis in HK‑2 cells through suppressing the PI3K/Akt pathway.

International journal of molecular medicine

He P,Lei J,Miao JN,Wu D,Wang C

MA1-081 was used in Western Blotting to investigate the role of the PI3K/Akt signaling pathway on HBx-induced renal tubular cell apoptosis.

Wed Apr 01 00:00:00 EDT 2020

Genome-wide identification of direct HBx genomic targets.

BMC genomics

Guerrieri F,Belloni L,D'Andrea D,Pediconi N,Le Pera L,Testoni B,Scisciani C,Floriot O,Zoulim F,Tramontano A,Levrero M

MA1-081 was used in Chromatin immunoprecipitation to analyse Hepatitis B virus recruitment on host cell chromatin in cells replicating Hepatitis B virus.

Fri Feb 17 00:00:00 EST 2017

HBx triggers either cellular senescence or cell proliferation depending on cellular phenotype.

Journal of viral hepatitis

Idrissi ME,Hachem H,Koering C,Merle P,Thénoz M,Mortreux F,Wattel E

MA1-081 was used in western blot to study HBx that triggers either cell proliferation orf cellular sensecence depending upon the phenotype of the cell

Mon Feb 01 00:00:00 EST 2016

Hepatitis B Virus X Protein Upregulates hELG1/ ATAD5 Expression through E2F1 in Hepatocellular Carcinoma.

International journal of biological sciences

Ghosh A,Ghosh S,Dasgupta D,Ghosh A,Datta S,Sikdar N,Datta S,Chowdhury A,Banerjee S

MA1-081 was used in ChIP assay and western blot to investigate how HBx alters hELG1/ATAD5 expression

Fri Sep 30 00:00:00 EDT 2016

Hepatitis B Virus X Protein Upregulates hELG1/ ATAD5 Expression through E2F1 in Hepatocellular Carcinoma.

International journal of biological sciences

Ghosh A,Ghosh S,Dasgupta D,Ghosh A,Datta S,Sikdar N,Datta S,Chowdhury A,Banerjee S

MA1-081 was used in ChIP assay and western blot to investigate how HBx alters hELG1/ATAD5 expression

Fri Sep 30 00:00:00 EDT 2016

Hepatitis B virus (HBV) induces the expression of interleukin-8 that in turn reduces HBV sensitivity to interferon-alpha.

Virology

Pollicino T,Bellinghieri L,Restuccia A,Raffa G,Musolino C,Alibrandi A,Teti D,Raimondo G

MA1-081 was used in western blot to study the mechanism by which HBV infection promotes the IL-8 production that reduces the sensitivity of the virus to IFN-alpha

Sun Sep 01 00:00:00 EDT 2013

HBx or HCV core gene expression in HepG2 human liver cells results in a survival benefit against oxidative stress with possible implications for HCC development.

Chemico-biological interactions

Severi T,Vander Borght S,Libbrecht L,VanAelst L,Nevens F,Roskams T,Cassiman D,Fevery J,Verslype C,van Pelt JF

MA1-081 was used in immunocytochemistry to study the protective effect of HBx or HCV core gene expression against oxidative stress in HepG2 cells

Sat Jun 30 00:00:00 EDT 2007

The hepatitis B virus X protein functionally interacts with CREB-binding protein/p300 in the regulation of CREB-mediated transcription.

The Journal of biological chemistry

Cougot D,Wu Y,Cairo S,Caramel J,Renard CA,Lévy L,Buendia MA,Neuveut C

MA1-081 was used in western blot to study the role of the hepatitis B virus X protein in the regulation of CREB-mediated transcription

Fri Feb 16 00:00:00 EST 2007

State of hepatitis C viral replication enhances activation of NF-kB- and AP-1-signaling induced by hepatitis B virus X.

Cancer letters

Kanda T,Yokosuka O,Nagao K,Saisho H

MA1-081 was used in western blot to study the possible involvement of hepatitis B virus X in HCV-related hepatocarcinogenesis

Tue Mar 28 00:00:00 EST 2006

Calpain-specific proteolysis in primate retina: Contribution of calpains in cell death.

Investigative ophthalmology & visual science

Nakajima E,David LL,Bystrom C,Shearer TR,Azuma M

MA1-081 was used in western blot to study the involvement of calpain-induced proteolysis in retinal cell death in primates

Fri Dec 01 00:00:00 EST 2006

Detection of hepatitis C virus RNA sequences in B-cell non-Hodgkin lymphoma.

American journal of clinical pathology

Karavattathayyil SJ,Kalkeri G,Liu HJ,Gaglio P,Garry RF,Krause JR,Dash S

MA1-081 was used in immunohistochemistry to investigate the role of hepatitis C virus in the pathogenesis of B-cell non-Hodgkin lymphomas

Wed Mar 01 00:00:00 EST 2000

Cloning and characterization of a novel hepatitis B virus x binding protein that inhibits viral replication.

Journal of virology

Melegari M,Scaglioni PP,Wands JR

MA1-081 was used in immunoprecipitation to identify and characterize a new hepatitis B virus x binding protein.

Sun Mar 01 00:00:00 EST 1998

Bioinformatics

Protein Aliases: HBV; HBVgp3; HepB; HepB X

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Hepatitis B Virus X Monoclonal Antibody (X36C)

Hepatitis B Virus X Antibody (MA1-081) in WB

Product Details

MA1-081

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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