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Invitrogen
Hepatitis C Virus NS5a Monoclonal Antibody (H110B)
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Hepatitis C Virus NS5a Antibody (MA1-7368) in WB
Western blot analysis using Hepatitis C Virus NS5a Antigen Monoclonal Antibody (Product # MA1-7368) was performed by loading 10 µg or 25 µg of whole cell extracts from human hepatoma cells (Huh7) or from Huh7 cells expressing a sub-genomic replicon of a hepatitis C virus (Huh7-Replicon). Membranes were probed with MA1-7368 (1:500), followed by a HRP-conjugated secondary antibody and chemiluminescent substra... View More
Product Details
MA1-7368
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
H110B
Immunogen
NS5a protein.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.1% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1017522
Product Specific Information
The MA1-7368 antibody reacts with Hepatitis C virus NS5a in viral and infected tissue samples. Positive for genotypes 1a and 1b, does not react with genotype 2a.
MA1-7368 has been successfully used in ELISA, Immunofluorescence, and Western blot applications.
The MA1-7368 antibody was raised against HCV NS5a protein.
Target Information
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HCV; HCV NS5; Hepatitis C Virus; HepC; HepC NS5; NICE-3
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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