Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
Histamine H1 Receptor (HRH1) Antibody (AHR-001-200UL) in IHC
Expression of Histamine H1receptor in mouse brain - Immunohistochemical staining of mouse ventromedial hypothalamus (VMH) using Anti-Histamine H1Receptor (HRH1) Antibody (#AHR-001). A. H1R (green fluorescence) appears in the outline of the VMH nucleus (arrows). B. DAPI (blue) counterstain labels all cell nuclei including VMH.
Product Details
AHR-001-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1, 3rd intracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Bordetella pertussis induced histamine sensitization; H1 receptor; H1R; HH1R; Histamine H1 receptor; Histamine H1-R; histamine receptor
Gene Aliases: Bphs; H1R; HH1R; Hir; Hisr; Hrh1
UniProt ID: (Mouse) P70174, (Rat) P31390
Entrez Gene ID: (Mouse) 15465, (Rat) 24448
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
chemical synaptic transmission
sensory perception of chemical stimulus
memory
visual learning
negative regulation of steroid biosynthetic process
regulation of vasoconstriction
regulation of vascular permeability
positive regulation of nitric oxide biosynthetic process
positive regulation of vasoconstriction
regulation of synaptic plasticity
eosinophil chemotaxis
rhythmic process
calcium ion import
calcium ion transmembrane transport
cellular response to histamine
manganese ion transmembrane transport
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
response to gravity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support