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Invitrogen
Histone H2A Recombinant Rabbit Monoclonal Antibody (RM225)
Product Details
MA5-24662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM225
Immunogen
Peptide corresponding to the C-terminus of human Histone H2A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4, with 50% glycerol, 1% BSA
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2661885
Product Specific Information
This antibody reacts to the Histone H2A protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyl-Histone H2; H2A 615 antibody; H2A GL101 antibody; H2A histone family member; H2A histone family, member A; H2A histone family, member C; H2A histone family, member E; H2A histone family, member G; H2A histone family, member M; H2A histone family, member P; H2A histone family, member R; H2A histone family, member X; H2A histone family, member Z; H2A-clustered histone 1; H2A-clustered histone 12; H2A.1; H2A.1 antibody; H2A/d; H2a/x; H2A/z; h2ad; H2ae; h2ai; H2ak; H2AK5ac; H2AX histone; H2AZ histone; H2B histone family, member U, (testis-specific); HIS; HIST1H2AE antibody; HIST3H2A; histone 1, H2aa; histone 1, H2ab; histone 1, H2ad; histone 1, H2ae; histone 1, H2ag; histone 1, H2ai; histone 1, H2aj; histone 1, H2ba; Histone H2A type 1; Histone H2A type 1-A; Histone H2A type 1-B/E; Histone H2A type 1-D; Histone H2A type 1-H; Histone H2A type 1-J; Histone H2A.2; Histone H2A.3; Histone H2A.X; Histone H2A.Z; Histone H2A/a; Histone H2A/e; Histone H2A/g; Histone H2A/m; histone H2A/p; Histone H2A/ptl; Histone H2A/r; Histone H2A/s; histone H2AD; histone H2AE; Histone H2AX; Histone H2B type 1-A; Histone H2B, testis; MGC74460 antibody; Testis-specific histone H2B; TSH2B.1
Gene Aliases: bA317E16.2; bA317E16.3; dJ160A22.4; dJ86C11.1; H2A.1; H2A.1b; H2A.2; H2A.3; H2A.X; H2A.z; H2A.Z-1; H2A/a; H2A/c; H2A/E; H2A/g; H2A/m; H2A/p; H2A/S; H2A/X; H2A/z; H2AA; H2AC1; H2AC11; H2AC12; H2AC13; H2AC14; H2AC15; H2AC16; H2AC17; H2AC4; H2AC7; H2AC8; H2AFA; H2AFALii; H2AFC; H2AFD; H2AFE; H2AFG; H2AFI; H2AFM; H2AFN; H2AFP; H2AFR; H2AFX; H2AFZ; H2AG; H2AH; H2AX; H2AZ; H2AZ1; H2BC1; H2BFU; HIST1H2AA; HIST1H2AB; HIST1H2AD; HIST1H2AE; HIST1H2AG; HIST1H2AH; HIST1H2AI; HIST1H2AJ; HIST1H2AK; HIST1H2AL; HIST1H2AM; HIST1H2BA; pH2A/f; STBP; TH2A; TH2B; TSH2B; TSH2B.1
UniProt ID: (Human) P16104, (Human) P0C0S5, (Human) Q96QV6, (Human) P04908, (Human) P20671, (Human) P0C0S8, (Human) Q96KK5, (Human) Q99878, (Human) Q96A08
Entrez Gene ID: (Human) 3014, (Human) 3015, (Human) 221613, (Human) 8335, (Human) 3013, (Human) 3012, (Human) 8969, (Human) 85235, (Human) 8329, (Human) 8331, (Human) 255626
DNA binding
damaged DNA binding
protein binding
enzyme binding
histone binding
protein heterodimerization activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin DNA binding
nucleosomal DNA binding
molecular_function
histone
DNA damage checkpoint
double-strand break repair via homologous recombination
double-strand break repair
double-strand break repair via nonhomologous end joining
nucleosome assembly
chromatin silencing
cellular response to DNA damage stimulus
spermatogenesis
response to ionizing radiation
viral process
cerebral cortex development
positive regulation of DNA repair
meiotic cell cycle
cellular response to gamma radiation
cellular senescence
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
negative regulation of cell proliferation
biological_process
nucleosome disassembly
inflammatory response
plasminogen activation
spermatogenesis, exchange of chromosomal proteins
positive regulation of binding
mononuclear cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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