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Histone H2B Antibody (MA5-31566) in WB
Western blot analysis of Histone H2B in various whole cell extracts using Histone H2B monoclonal antibody (Product # MA5-31566) using 30 µg of sample at a dilution of 1:100. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
Product Details
MA5-31566
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
GT387
Immunogen
Full length human Histone H2B recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787193
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (96%), Bovine (100%).
Positive Control: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyl-Histone H2; H2B GL105 antibody; H2B histone family member W testis-specific; H2B histone family, member A; H2B histone family, member B; H2B histone family, member C; H2B histone family, member D; H2B histone family, member E; H2B histone family, member F; H2B histone family, member J; H2B histone family, member K; H2B histone family, member L; H2B histone family, member M; H2B histone family, member N; H2B histone family, member Q; H2B histone family, member R; H2B histone family, member T; H2B histone family, member U, (testis-specific); H2B histone family, member W, testis-specific; H2B K; H2B type 12; H2B-clustered histone 12 like; H2B-clustered histone 17; H2B-clustered histone 18; H2B-clustered histone 21; H2B-clustered histone 26; H2B-clustered histone 3; H2B-clustered histone 5; H2B-clustered histone 9; H2B.1 antibody; H2B.S histone 1; H2B.U histone 1; H2B.W histone 1; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/g; H2B/h; H2B/I; H2B/j; H2B/k; H2B/l; H2B/n; H2B/q; H2B/r; H2B/s; H2BK12ac; H2BK15ac; H2BK5ac; H2BS14; H2BS14p; HIRA-interacting protein 1; HIRA-interacting protein 2; HIST1H2BB antibody; HIST1H3I; histone 1, H2ba; histone 1, H2bb; histone 1, H2bc; histone 1, H2bd; histone 1, H2bg; histone 1, H2bh; histone 1, H2bi; histone 1, H2bj; histone 1, H2bk; histone 1, H2bl; histone 1, H2bm; histone 1, H2bn; histone 1, H2bo; histone 2, H2be; Histone 2B; histone 3, H2bb; histone family member; Histone H2B type 1-A; Histone H2B type 1-B; Histone H2B type 1-C/E/F/G/I; Histone H2B type 1-D; Histone H2B type 1-H; Histone H2B type 1-J; Histone H2B type 1-K; Histone H2B type 1-L; Histone H2B type 1-M; Histone H2B type 1-N; Histone H2B type 1-O; Histone H2B type 2-E; Histone H2B type 2-F; Histone H2B type 3-B; Histone H2B type F-M; Histone H2B type F-S; histone H2B type F-S-like; Histone H2B type W-T; Histone H2B, testis; Histone H2B-GL105; Histone H2B.1; Histone H2B.1 A; Histone H2B.1 B; Histone H2B.2; Histone H2B.a; Histone H2B.b; Histone H2B.c; Histone H2B.d; Histone H2B.e; Histone H2B.f; Histone H2B.g; Histone H2B.h; Histone H2B.I; Histone H2B.j; Histone H2B.k; Histone H2B.l; Histone H2B.n; Histone H2B.q; Histone H2B.r; Histone H2B.s; MGC125414; MGC125415; MGC125416; MGC9388; RP1-193B12.10; Testis-specific histone H2B; TSH2B.1
Gene Aliases: bA317E16.3; dJ160A22.3; dJ193B12.2; dJ221C16.3; dJ221C16.6; dJ221C16.8; dJ97D16.4; GL105; H2B; H2B.1; H2B.1A; H2B.1B; H2B.2; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/j; H2B/k; H2B/l; H2B/n; H2B/r; H2B/S; H2Bb; H2BC1; H2BC10; H2BC11; H2BC12; H2BC12L; H2BC13; H2BC14; H2BC15; H2BC17; H2BC18; H2BC21; H2BC26; H2BC3; H2BC4; H2BC5; H2BC6; H2BC7; H2BC8; H2BC9; H2BFA; H2BFAiii; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFG; H2BFH; H2BFJ; H2BFK; H2BFL; H2BFM; H2BFN; H2BFQ; H2BFR; H2BFS; H2BFT; H2BFU; H2BFWT; H2BGL105; H2BJ; H2BK; H2BQ; H2BS1; H2BU1; H2BW1; H2BW2; HIRIP1; HIRIP2; HIST1H2BA; HIST1H2BB; HIST1H2BC; HIST1H2BD; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BH; HIST1H2BI; HIST1H2BJ; HIST1H2BK; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST1H2BO; HIST2H2BE; HIST2H2BF; HIST3H2BB; STBP; TH2B; TH2B-175; TSH2B; TSH2B.1
nucleosome assembly
nucleosome disassembly
inflammatory response
plasminogen activation
spermatogenesis, exchange of chromosomal proteins
positive regulation of binding
mononuclear cell migration
innate immune response in mucosa
antibacterial humoral response
defense response to Gram-positive bacterium
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