Invitrogen

Histone H3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 Published Figures

20 References

View all (196) Histone H3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Histone H3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Histone H3 Antibody (PA5-16183) in ICC/IF

Immunofluorescence analysis of Histone H3 was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone H3 Rabbit Polyclonal Antibody (Product # PA5-16183) at 1:250 dilution in 0.1% BSA and incubated for 3... View More

Histone H3 Antibody in ChIP Assay (ChIP)

Histone H3 Antibody in Western Blot (WB)

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Product Details

PA5-16183

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 15 publications 15 publications

Immunocytochemistry (ICC/IF)

1:250

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

1:50

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Xenopus, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the carboxy-terminal sequence of human histone H3

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

555 µg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985434

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other histones.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes.

Nature communications

Djakovic L,Hennig T,Reinisch K,Milić A,Whisnant AW,Wolf K,Weiß E,Haas T,Grothey A,Jürges CS,Kluge M,Wolf E,Erhard F,Friedel CC,Dölken L

PA5-16183 was used in Immunoprecipitation to propose a model in which allosteric changes in Pol II composition downstream of genes and ICP22-mediated interference with FACT activity explain the differential impairment of histone repositioning downstream of genes in the wake of Pol II in HSV-1 infection.

Mon Jul 31 00:00:00 EDT 2023

Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease.

The Journal of pathology

Carrascal M,Areny-Balagueró A,de-Madaria E,Cárdenas-Jaén K,García-Rayado G,Rivera R,Martin Mateos RM,Pascual-Moreno I,Gironella M,Abian J,Closa D

PA5-16183 was used in Western Blotting to find that the final severity of the disease strongly correlates with the inflammatory capacity of exosomes in the early stages of acute pancreatitis.

Sat Jan 01 00:00:00 EST 2022

Proteoglycan 4 (PRG4) treatment enhances wound closure and tissue regeneration.

NPJ Regenerative medicine

Krawetz RJ,Abubacker S,Leonard C,Masson AO,Shah S,Narendran N,Tailor P,Regmi SC,Labit E,Ninkovic N,Corpuz JM,Ito K,Underhill TM,Salo PT,Schmidt TA,Biernaskie JA

PA5-16183 was used in Western Blotting to propose that rhPRG4 may represent a potential therapy to mitigate scar and improve wound healing.

Fri Jun 24 00:00:00 EDT 2022

BRD4 promotes resection and homology-directed repair of DNA double-strand breaks.

Nature communications

Barrows JK,Lin B,Quaas CE,Fullbright G,Wallace EN,Long DT

PA5-16183 was used in Immunoprecipitation to establish BRD4 as a multifunctional regulator of chromatin binding that links transcriptional activity and homology-directed repair.

Tue May 31 00:00:00 EDT 2022

Short homology-directed repair using optimized Cas9 in the pathogen Cryptococcus neoformans enables rapid gene deletion and tagging.

Genetics

Huang MY,Joshi MB,Boucher MJ,Lee S,Loza LC,Gaylord EA,Doering TL,Madhani HD

PA5-16183 was used in Western Blotting to find that a Cas9 harboring only common C. neoformans codons and a consensus C. neoformans intron together with a TEF1 promoter and terminator and a nuclear localization signal (Cno CAS9 or "CnoCAS9") reliably enabled genome editing in the widely used KN99α C. neoformans strain.

Tue Jan 04 00:00:00 EST 2022

BRCA1-BARD1 regulates transcription through BRD4 in Xenopus nucleoplasmic extract.

Nucleic acids research

Barrows JK,Fullbright G,Long DT

Published figure using Histone H3 polyclonal antibody (Product # PA5-16183) in ChIP assay

Tue Apr 06 00:00:00 EDT 2021

MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.

Neural regeneration research

Zou Z,Dong YS,Liu DD,Li G,Hao GZ,Gao X,Pan PY,Liang GB

PA5-16183 was used in Western Blotting to investigate whether MAP4K4 plays a role in the pathophysiology of subarachnoid hemorrhage.

Mon Feb 01 00:00:00 EST 2021

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.

International journal of molecular sciences

Sakowicz A,Bralewska M,Pietrucha T,Figueras F,Habrowska-Górczyńska DE,Piastowska-Ciesielska AW,Gach A,Sakowicz B,Rybak-Krzyszkowska M,Huras H,Grzesiak M,Biesiada L

Published figure using Histone H3 polyclonal antibody (Product # PA5-16183) in Western Blot

Wed Sep 22 00:00:00 EDT 2021

The Aspartic Protease Ddi1 Contributes to DNA-Protein Crosslink Repair in Yeast.

Molecular cell

Serbyn N,Noireterre A,Bagdiul I,Plank M,Michel AH,Loewith R,Kornmann B,Stutz F

PA5-16183 was used in Western Blotting to describe that the efficient DPC disassembly requires Ddi1, another conserved predicted protease in Saccharomyces cerevisiae.

Thu Mar 05 00:00:00 EST 2020

Proceedings of the National Academy of Sciences of the United States of America

White CW,Fan X,Maynard JC,Wheatley EG,Bieri G,Couthouis J,Burlingame AL,Villeda SA

PA5-16183 was used in Western Blotting to report that age-related loss of the posttranslational modification, O-linked β-N-acetylglucosamine (O-GlcNAc), in NSCs promotes a glial fate switch.

Tue Sep 08 00:00:00 EDT 2020

Knockdown of DNA-binding protein A enhances the chemotherapy sensitivity of colorectal cancer via suppressing the Wnt/β-catenin/Chk1 pathway.

Cell biology international

Tong C,Qu K,Wang G,Liu R,Duan B,Wang X,Liu C

PA5-16183 was used in Western Blotting to indicate that knockdown of dbpA enhances sensitivity of CRC cells to 5-FU via Wnt/β-catenin/Chk1 pathway.

Thu Oct 01 00:00:00 EDT 2020

Knockdown of DNA-binding protein A enhances the chemotherapy sensitivity of colorectal cancer via suppressing the Wnt/β-catenin/Chk1 pathway.

Cell biology international

Tong C,Qu K,Wang G,Liu R,Duan B,Wang X,Liu C

PA5-16183 was used in Western Blotting to indicate that knockdown of dbpA enhances sensitivity of CRC cells to 5-FU via Wnt/β-catenin/Chk1 pathway.

Thu Oct 01 00:00:00 EDT 2020

Intronic Determinants Coordinate Charme lncRNA Nuclear Activity through the Interaction with MATR3 and PTBP1.

Cell reports

Desideri F,Cipriano A,Petrezselyova S,Buonaiuto G,Santini T,Kasparek P,Prochazka J,Janson G,Paiardini A,Calicchio A,Colantoni A,Sedlacek R,Bozzoni I,Ballarino M

PA5-16183 was used in Western Blotting to identify the proteins that assist these chromatin activities.

Tue Dec 22 00:00:00 EST 2020

Extra-mitochondrial mouse frataxin and its implications for mouse models of Friedreich's ataxia.

Scientific reports

Weng L,Laboureur L,Wang Q,Guo L,Xu P,Gottlieb L,Lynch DR,Mesaros C,Blair IA

PA5-16183 was used in Western Blotting to demonstrate the role of extra-mitochondrial frataxin in the etiology of Friedreich's ataxia.

Fri Sep 25 00:00:00 EDT 2020

Mitochondrial connexin 43 in sex-dependent myocardial responses and estrogen-mediated cardiac protection following acute ischemia/reperfusion injury.

Basic research in cardiology

Wang M,Smith K,Yu Q,Miller C,Singh K,Sen CK

PA5-16183 was used in Western Blotting to determine the implication of mitochondrial Cx43 in sex-related myocardial responses and to examine the effect of estrogen (17β-estradiol, E2) on Cx43, particularly mitochondrial Cx43-involved cardiac protection following I/R.

Mon Nov 18 00:00:00 EST 2019

Uncoupling of p97 ATPase activity has a dominant negative effect on protein extraction.

Scientific reports

Rycenga HB,Wolfe KB,Yeh ES,Long DT

PA5-16183 was used in Western Blotting to study how pathogenic mutations in p97 alter its cellular function.

Wed Jul 17 00:00:00 EDT 2019

Cell-free transcription in Xenopus egg extract.

The Journal of biological chemistry

Barrows JK,Long DT

PA5-16183 was used in Immunodepletion to understand how Xenopus egg extract can be used to create a cell-free system to regulate transcription activation during development.

Fri Dec 20 00:00:00 EST 2019

Modulation of Endocannabinoid-Binding Receptors in Human Neuroblastoma Cells by Tunicamycin.

Molecules (Basel, Switzerland)

Rapino C,Castellucci A,Lizzi AR,Sabatucci A,Angelucci CB,Tortolani D,Rossi G,D'Andrea G,Maccarrone M

PA5-16183 was used in Western Blotting to support N-linked glycosylation as an unprecedented post-translational modification that may modulate eCB-binding receptors' expression and localization, in particular for CB₁.

Thu Apr 11 00:00:00 EDT 2019

Brr6 plays a role in gene recruitment and transcriptional regulation at the nuclear envelope.

Molecular biology of the cell

de Bruyn Kops A,Burke JE,Guthrie C

PA5-16183 was used in Chromatin immunoprecipitation to argue that Brr6 interacts with chromatin, helping to maintain normal chromatin architecture and transcriptional regulation of certain loci at the nuclear envelope.

Mon Oct 15 00:00:00 EDT 2018

A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division.

eLife

Li Y,Liu D,López-Paz C,Olson BJ,Umen JG

Published figure using Histone H3 polyclonal antibody (Product # PA5-16183) in Western Blot

Fri Mar 25 00:00:00 EDT 2016

Substance P induces CCN1 expression via histone deacetylase activity in human colonic epithelial cells.

The American journal of pathology

Koon HW,Shih DQ,Hing TC,Chen J,Ho S,Zhao D,Targan SR,Pothoulakis C

Published figure using Histone H3 polyclonal antibody (Product # PA5-16183) in Western Blot

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3S10ph; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H3a; Histone Cluster 2 H3a; histone cluster 2, H3c2, pseudogene; histone cluster 3, H3; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Mouse) Q8CGN9

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Rat) 679994, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion histone

Process(es)

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

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Histone H3 Polyclonal Antibody

Histone H3 Antibody (PA5-16183) in ICC/IF

Product Details

PA5-16183

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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