Active Motif

Histone H3 Monoclonal Antibody (MABI 0301)

Histone H3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Histone H3 Antibody (39763) in ICC/IF

Histone H3 antibody (mAb) tested by immunofluorescence. HeLa cells stained with Histone H3 antibody (mAb) at a dilution of 1 µg/mL.

Histone H3 Antibody in Western Blot (WB)

Histone H3 Antibody in ChIP Assay (ChIP)

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Product Details

39763

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

ChIP assay (ChIP)

5-10 µg

ChIP-sequencing (ChIP-Seq)

4 µg

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Fruit fly, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MABI 0301

Immunogen

This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.5, with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2650522

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide.

The Journal of organic chemistry

Trebacz M,Wang Y,Makotta L,Henschke L,Köhn M

Research topic: Histone Methylation

Fri Feb 07 00:00:00 EST 2020

Oscillatory cAMP signaling rapidly alters H3K4 methylation.

Life science alliance

Huff TC,Camarena V,Sant DW,Wilkes Z,Van Booven D,Aron AT,Muir RK,Renslo AR,Chang CJ,Monje PV,Wang G

Research topic: Histone Methylation

Wed Jan 01 00:00:00 EST 2020

A CRISPR/Cas9 screen identifies the histone demethylase MINA53 as a novel HIV-1 latency-promoting gene (LPG).

Nucleic acids research

Huang H,Kong W,Jean M,Fiches G,Zhou D,Hayashi T,Que J,Santoso N,Zhu J

Research topic: CRISPR, HIV

Thu Aug 22 00:00:00 EDT 2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Alcoholism, clinical and experimental research

Jarmasz JS,Stirton H,Basalah D,Davie JR,Clarren SK,Astley SJ,Del Bigio MR

Research topic: Neurobiology, Alcoholism

Sat Jun 01 00:00:00 EDT 2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Alcoholism, clinical and experimental research

Jarmasz JS,Stirton H,Basalah D,Davie JR,Clarren SK,Astley SJ,Del Bigio MR

Research topic: Neurobiology, Alcoholism

Sat Jun 01 00:00:00 EDT 2019

Gonadal supporting cells acquire sex-specific chromatin landscapes during mammalian sex determination.

Developmental biology

Garcia-Moreno SA,Futtner CR,Salamone IM,Gonen N,Lovell-Badge R,Maatouk DM

Research topic: Gene Regulation, Developmental Biology

Fri Feb 15 00:00:00 EST 2019

Iws1 and Spt6 Regulate Trimethylation of Histone H3 on Lysine 36 through Akt Signaling and are Essential for Mouse Embryonic Genome Activation.

Scientific reports

Oqani RK,Lin T,Lee JE,Kang JW,Shin HY,Il Jin D

Research topic: Embryonic development, Gene regulation

Thu Mar 07 00:00:00 EST 2019

Quantitative Multiplexed ChIP Reveals Global Alterations that Shape Promoter Bivalency in Ground State Embryonic Stem Cells.

Cell reports

Kumar B,Elsässer SJ

Research topic: Stem Cells

Tue Sep 17 00:00:00 EDT 2019

The TGFB2-AS1 lncRNA Regulates TGF-β Signaling by Modulating Corepressor Activity.

Cell reports

Papoutsoglou P,Tsubakihara Y,Caja L,Morén A,Pallis P,Ameur A,Heldin CH,Moustakas A

Research topic: Gene Regulation, Transcriptomics

Tue Sep 17 00:00:00 EDT 2019

CAPRI enables comparison of evolutionarily conserved RNA interacting regions.

Nature communications

Panhale A,Richter FM,Ramírez F,Shvedunova M,Manke T,Mittler G,Akhtar A

Research topic: RNA

Tue Jun 18 00:00:00 EDT 2019

The tumor suppressor menin prevents effector CD8 T-cell dysfunction by targeting mTORC1-dependent metabolic activation.

Nature communications

Suzuki J,Yamada T,Inoue K,Nabe S,Kuwahara M,Takemori N,Takemori A,Matsuda S,Kanoh M,Imai Y,Yasukawa M,Yamashita M

Research topic: Immunology & Hematology

Fri Aug 17 00:00:00 EDT 2018

Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure.

Cell discovery

El-Daher MT,Cagnard N,Gil M,Da Cruz MC,Leveau C,Sepulveda F,Zarhrate M,Tores F,Legoix P,Baulande S,de Villartay JP,Almouzni G,Quivy JP,Fischer A,de Saint Basile G

Research topic: Transcription, Chromatin Structure

Thu Oct 01 00:00:00 EDT 2020

KLF5 controls glutathione metabolism to suppress p190-BCR-ABL+ B-cell lymphoblastic leukemia.

Oncotarget

Zhang C,D'Alessandro A,Wellendorf AM,Mohmoud F,Serrano-Lopez J,Perentesis JP,Komurov K,Alexe G,Stegmaier K,Whitsett JA,Grimes HL,Cancelas JA

Research topic: Devepmental Biology

Tue Jul 03 00:00:00 EDT 2018

The ZZ-type zinc finger of ZZZ3 modulates the ATAC complex-mediated histone acetylation and gene activation.

Nature communications

Mi W,Zhang Y,Lyu J,Wang X,Tong Q,Peng D,Xue Y,Tencer AH,Wen H,Li W,Kutateladze TG,Shi X

Research topic: Gene Regulation

Fri Sep 14 00:00:00 EDT 2018

Transcriptional repression by FACT is linked to regulation of chromatin accessibility at the promoter of ES cells.

Life science alliance

Mylonas C,Tessarz P

Research topic: Diabetes

Fri Jun 01 00:00:00 EDT 2018

Cytoplasmic chromatin triggers inflammation in senescence and cancer.

Nature

Dou Z,Ghosh K,Vizioli MG,Zhu J,Sen P,Wangensteen KJ,Simithy J,Lan Y,Lin Y,Zhou Z,Capell BC,Xu C,Xu M,Kieckhaefer JE,Jiang T,Shoshkes-Carmel M,Tanim KMAA,Barber GN,Seykora JT,Millar SE,Kaestner KH,Garcia BA,Adams PD,Berger SL

Research topic: Immunology & Hematology

Thu Oct 19 00:00:00 EDT 2017

Prognostic value of histone chaperone FACT subunits expression in breast cancer.

Breast cancer (Dove Medical Press)

Attwood K,Fleyshman D,Prendergast L,Paszkiewicz G,Omilian AR,Bshara W,Gurova K

Research topic: Breast Cancer

Thu Oct 01 00:00:00 EDT 2020

Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation.

Cell reports

Bulusu V,Tumanov S,Michalopoulou E,van den Broek NJ,MacKay G,Nixon C,Dhayade S,Schug ZT,Vande Voorde J,Blyth K,Gottlieb E,Vazquez A,Kamphorst JJ

Research topic: Transcription Regulation

Tue Jan 17 00:00:00 EST 2017

Chromatin condensation of Xist genomic loci during oogenesis in mice.

Development (Cambridge, England)

Fukuda A,Mitani A,Miyashita T,Umezawa A,Akutsu H

Research topic: Oogenesis

Tue Dec 01 00:00:00 EST 2015

Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery.

Epigenetics & chromatin

Zhan Y,Kost-Alimova M,Shi X,Leo E,Bardenhagen JP,Shepard HE,Appikonda S,Vangamudi B,Zhao S,Tieu TN,Jiang S,Heffernan TP,Marszalek JR,Toniatti C,Draetta G,Tyler J,Barton M,Jones P,Palmer WS,Geck Do MK,Andersen JN

Research topic: Bromodomains, Drug Discovery

Wed Sep 23 00:00:00 EDT 2015

Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery.

Epigenetics & chromatin

Research topic: Bromodomains, Drug Discovery

Wed Sep 23 00:00:00 EDT 2015

L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation.

Cell communication and signaling : CCS

Wagner W,Ciszewski WM,Kania KD

Research topic: Cervical Cancer

Sat Jul 25 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3S10ph; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone Cluster 2 H3a; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o

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Gene Aliases: H3; H3.2; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D

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UniProt ID: (Human) P68431, (Human) Q71DI3

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion histone

Process(es)

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Histone H3 Monoclonal Antibody (MABI 0301)

Histone H3 Antibody (39763) in ICC/IF

Product Details

39763

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

in situ PLA (PLA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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