Active Motif

Histone H3K27me3 Polyclonal Antibody

Histone H3K27me3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Histone H3K27me3 Antibody (39155) in ICC/IF

Histone H3 trimethyl Lys27 antibody tested by immunofluorescence. HeLa cells stained with Histone H3 trimethyl Lys27 antibody (1.7 µg/mL dilution). Top panel: Histone H3 trimethyl Lys27 antibody. Middle: DAPI. Bottom: Merge of both images.

Histone H3K27me3 Antibody in Western Blot (WB)

Histone H3K27me3 Antibody in ChIP-Sequencing (ChIP-Seq)

Histone H3K27me3 Antibody in ELISA (ELISA)

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Product Details

39155

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

5-10 µg

View 3 publications 3 publications

ChIP-sequencing (ChIP-Seq)

5 µg

View 11 publications 11 publications

Dot blot (DB)

Assay-dependent

Miscellaneous PubMed (Misc)

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

C. elegans, Fruit fly, Human, Invertebrate, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Histone H3 trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3. testing

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Datasheet

Purification

Protein A

Storage buffer

PBS, pH 7.5, with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2561020

Target Information

H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A CRISPR/Cas9 screen identifies the histone demethylase MINA53 as a novel HIV-1 latency-promoting gene (LPG).

Nucleic acids research

Huang H,Kong W,Jean M,Fiches G,Zhou D,Hayashi T,Que J,Santoso N,Zhu J

Research topic: CRISPR, HIV

Thu Aug 22 00:00:00 EDT 2019

MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications.

Nature protocols

Zhu B,Hsieh YP,Murphy TW,Zhang Q,Naler LB,Lu C

Research topic: ChIP Methods

Sun Dec 01 00:00:00 EST 2019

MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications.

Nature protocols

Zhu B,Hsieh YP,Murphy TW,Zhang Q,Naler LB,Lu C

Research topic: ChIP Methods

Sun Dec 01 00:00:00 EST 2019

The role of Xist-mediated Polycomb recruitment in the initiation of X-chromosome inactivation.

EMBO reports

Bousard A,Raposo AC,Żylicz JJ,Picard C,Pires VB,Qi Y,Gil C,Syx L,Chang HY,Heard E,da Rocha ST

Research topic: Gene Silencing

Fri Oct 04 00:00:00 EDT 2019

Epigenetic biomarker screening by FLIM-FRET for combination therapy in ER+ breast cancer.

Clinical epigenetics

Liu W,Cui Y,Ren W,Irudayaraj J

Research topic: Breast Cancer

Wed Jan 30 00:00:00 EST 2019

TET1 regulates fibroblast growth factor 8 transcription in gonadotropin releasing hormone neurons.

PloS one

Linscott ML,Chung WCJ

Research topic: Kallmann Syndrome (KS), Gene Regulation

Tue Mar 10 00:00:00 EDT 2020

IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP.

eLife

Roy A,Ghosh A,Kumar B,Chandran B

Research topic: Virology, KSHV

Mon Nov 04 00:00:00 EST 2019

Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses.

Cancer cell

Stewart E,McEvoy J,Wang H,Chen X,Honnell V,Ocarz M,Gordon B,Dapper J,Blankenship K,Yang Y,Li Y,Shaw TI,Cho JH,Wang X,Xu B,Gupta P,Fan Y,Liu Y,Rusch M,Griffiths L,Jeon J,Freeman BB,Clay MR,Pappo A,Easton J,Shurtleff S,Shelat A,Zhou X,Boggs K,Mulder H,Yergeau D,Bahrami A,Mardis ER,Wilson RK,Zhang J,Peng J,Downing JR,Dyer MA

Research topic: Skeletal Muscle - Rhabdomyosarcoma

Mon Sep 10 00:00:00 EDT 2018

Disruption of CTCF-YY1-dependent looping of the human papillomavirus genome activates differentiation-induced viral oncogene transcription.

PLoS biology

Pentland I,Campos-León K,Cotic M,Davies KJ,Wood CD,Groves IJ,Burley M,Coleman N,Stockton JD,Noyvert B,Beggs AD,West MJ,Roberts S,Parish JL

Research topic: Virology (HPV)

Mon Oct 01 00:00:00 EDT 2018

Diversity of Epigenetic Features of the Inactive X-Chromosome in NK Cells, Dendritic Cells, and Macrophages.

Frontiers in immunology

Syrett CM,Sindhava V,Sierra I,Dubin AH,Atchison M,Anguera MC

Research topic: Chromosome Biology

Mon Oct 21 00:00:00 EDT 2019

SIRT1 mediates obesity- and nutrient-dependent perturbation of pubertal timing by epigenetically controlling Kiss1 expression.

Nature communications

Vazquez MJ,Toro CA,Castellano JM,Ruiz-Pino F,Roa J,Beiroa D,Heras V,Velasco I,Dieguez C,Pinilla L,Gaytan F,Nogueiras R,Bosch MA,Rønnekleiv OK,Lomniczi A,Ojeda SR,Tena-Sempere M

Research topic: Mammalian puberty & sexual development

Wed Oct 10 00:00:00 EDT 2018

Integrative epigenetic taxonomy of primary prostate cancer.

Nature communications

Stelloo S,Nevedomskaya E,Kim Y,Schuurman K,Valle-Encinas E,Lobo J,Krijgsman O,Peeper DS,Chang SL,Feng FY,Wessels LFA,Henrique R,Jerónimo C,Bergman AM,Zwart W

Research topic: Prostate Cancer

Wed Nov 21 00:00:00 EST 2018

Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.

Nature medicine

Jin X,Kim LJY,Wu Q,Wallace LC,Prager BC,Sanvoranart T,Gimple RC,Wang X,Mack SC,Miller TE,Huang P,Valentim CL,Zhou QG,Barnholtz-Sloan JS,Bao S,Sloan AE,Rich JN

Research topic: Brain & Nerves

Wed Nov 01 00:00:00 EDT 2017

Repression of Stress-Induced LINE-1 Expression Protects Cancer Cell Subpopulations from Lethal Drug Exposure.

Cancer cell

Guler GD,Tindell CA,Pitti R,Wilson C,Nichols K,KaiWai Cheung T,Kim HJ,Wongchenko M,Yan Y,Haley B,Cuellar T,Webster J,Alag N,Hegde G,Jackson E,Nance TL,Giresi PG,Chen KB,Liu J,Jhunjhunwala S,Settleman J,Stephan JP,Arnott D,Classon M

Research topic: Cancer

Mon Aug 14 00:00:00 EDT 2017

Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers.

Nucleic acids research

Zarnegar MA,Reinitz F,Newman AM,Clarke MF

Research topic: Methods

Fri Sep 29 00:00:00 EDT 2017

The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis.

Neuron

Aldiri I,Xu B,Wang L,Chen X,Hiler D,Griffiths L,Valentine M,Shirinifard A,Thiagarajan S,Sablauer A,Barabas ME,Zhang J,Johnson D,Frase S,Zhou X,Easton J,Zhang J,Mardis ER,Wilson RK,Downing JR,Dyer MA

Research topic: Eyes

Wed May 03 00:00:00 EDT 2017

Associations between post translational histone modifications, myelomeningocele risk, environmental arsenic exposure, and folate deficiency among participants in a case control study in Bangladesh.

Epigenetics

Tauheed J,Sanchez-Guerra M,Lee JJ,Paul L,Ibne Hasan MOS,Quamruzzaman Q,Selhub J,Wright RO,Christiani DC,Coull BA,Baccarelli AA,Mazumdar M

Research topic: Envinronmental Health

Sat Jun 03 00:00:00 EDT 2017

H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis.

Nature communications

Zhang X,Li B,Rezaeian AH,Xu X,Chou PC,Jin G,Han F,Pan BS,Wang CY,Long J,Zhang A,Huang CY,Tsai FJ,Tsai CH,Logothetis C,Lin HK

Research topic: Cancer

Thu Mar 16 00:00:00 EDT 2017

EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jκ and EBF1.

PLoS pathogens

Lu F,Chen HS,Kossenkov AV,DeWispeleare K,Won KJ,Lieberman PM

Research topic: Epstein-Barr Virus (EBV)

Fri Jan 01 00:00:00 EST 2016

HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus.

Journal of virology

Dheekollu J,Wiedmer A,Sentana-Lledo D,Cassel J,Messick T,Lieberman PM

Research topic: Virology - EBV

Wed Jun 01 00:00:00 EDT 2016

Alterations in nuclear structure promote lupus autoimmunity in a mouse model.

Disease models & mechanisms

Singh N,Johnstone DB,Martin KA,Tempera I,Kaplan MJ,Denny MF

Research topic: Autoimmunity - Lupus (SLE)

Mon Aug 01 00:00:00 EDT 2016

Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An in vitro study.

World journal of gastroenterology

Das D,Sarkar N,Sengupta I,Pal A,Saha D,Bandopadhyay M,Das C,Narayan J,Singh SP,Chakravarty R

Research topic: Hepatitis B

Wed Dec 21 00:00:00 EST 2016

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Biology open

Carofino BL,Ayanga B,Tracey LJ,Brooke-Bisschop T,Justice MJ

Research topic: Leukemia

Sun May 15 00:00:00 EDT 2016

Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification.

F1000Research

Mendoza-Parra MA,Saravaki V,Cholley PE,Blum M,Billoré B,Gronemeyer H

Mon Jun 27 00:00:00 EDT 2016

Reader domain specificity and lysine demethylase-4 family function.

Nature communications

Su Z,Wang F,Lee JH,Stephens KE,Papazyan R,Voronina E,Krautkramer KA,Raman A,Thorpe JJ,Boersma MD,Kuznetsov VI,Miller MD,Taverna SD,Phillips GN,Denu JM

Research topic: Cell Cycle, Meiosis

Mon Nov 14 00:00:00 EST 2016

Sex Specification and Heterogeneity of Primordial Germ Cells in Mice.

PloS one

Sakashita A,Kawabata Y,Jincho Y,Tajima S,Kumamoto S,Kobayashi H,Matsui Y,Kono T

Research topic: Embryogenesis

Thu Jun 30 00:00:00 EDT 2016

DNA Methylation in the Exon 1 Region and Complex Regulation of Twist1 Expression in Gastric Cancer Cells.

PloS one

Sakamoto A,Akiyama Y,Shimada S,Zhu WG,Yuasa Y,Tanaka S

Research topic: Gastric Cancer

Thu Jun 23 00:00:00 EDT 2016

Tumor-secreted Hsp90 subverts polycomb function to drive prostate tumor growth and invasion.

The Journal of biological chemistry

Nolan KD,Franco OE,Hance MW,Hayward SW,Isaacs JS

Research topic: Prostate Cancer

Fri Mar 27 00:00:00 EDT 2015

Reconfiguration of nucleosome-depleted regions at distal regulatory elements accompanies DNA methylation of enhancers and insulators in cancer.

Genome research

Taberlay PC,Statham AL,Kelly TK,Clark SJ,Jones PA

Research topic: DNA hypermethylation

Mon Sep 01 00:00:00 EDT 2014

Application of histone modification-specific interaction domains as an alternative to antibodies.

Genome research

Kungulovski G,Kycia I,Tamas R,Jurkowska RZ,Kudithipudi S,Henry C,Reinhardt R,Labhart P,Jeltsch A

Sat Nov 01 00:00:00 EDT 2014

Application of histone modification-specific interaction domains as an alternative to antibodies.

Genome research

Kungulovski G,Kycia I,Tamas R,Jurkowska RZ,Kudithipudi S,Henry C,Reinhardt R,Labhart P,Jeltsch A

Sat Nov 01 00:00:00 EDT 2014

Application of histone modification-specific interaction domains as an alternative to antibodies.

Genome research

Kungulovski G,Kycia I,Tamas R,Jurkowska RZ,Kudithipudi S,Henry C,Reinhardt R,Labhart P,Jeltsch A

Sat Nov 01 00:00:00 EDT 2014

Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.

Development (Cambridge, England)

Kumar S,Duester G

Research topic: Embryogenesis

Fri Aug 01 00:00:00 EDT 2014

NFIB-mediated repression of the epigenetic factor Ezh2 regulates cortical development.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Piper M,Barry G,Harvey TJ,McLeay R,Smith AG,Harris L,Mason S,Stringer BW,Day BW,Wray NR,Gronostajski RM,Bailey TL,Boyd AW,Richards LJ

Research topic: Neurobiology, Cortical Development

Wed Feb 19 00:00:00 EST 2014

The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation.

Epigenetics & chromatin

Minkovsky A,Sahakyan A,Rankin-Gee E,Bonora G,Patel S,Plath K

Research topic: Embryogenesis, X chromosome inactivation (XCI)

Wed Jul 16 00:00:00 EDT 2014

The roles of co-chaperone CCRP/DNAJC7 in Cyp2b10 gene activation and steatosis development in mouse livers.

PloS one

Ohno M,Kanayama T,Moore R,Ray M,Negishi M

Research topic: Liver

Mon Aug 31 00:00:00 EDT 2015

Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis.

eLife

Papazyan R,Voronina E,Chapman JR,Luperchio TR,Gilbert TM,Meier E,Mackintosh SG,Shabanowitz J,Tackett AJ,Reddy KL,Coyne RS,Hunt DF,Liu Y,Taverna SD

Research topic: DNA Damage, Meiosis

Tue Aug 26 00:00:00 EDT 2014

Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis.

eLife

Papazyan R,Voronina E,Chapman JR,Luperchio TR,Gilbert TM,Meier E,Mackintosh SG,Shabanowitz J,Tackett AJ,Reddy KL,Coyne RS,Hunt DF,Liu Y,Taverna SD

Research topic: DNA Damage, Meiosis

Tue Aug 26 00:00:00 EDT 2014

Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells.

Proceedings of the National Academy of Sciences of the United States of America

Murphy PJ,Cipriany BR,Wallin CB,Ju CY,Szeto K,Hagarman JA,Benitez JJ,Craighead HG,Soloway PD

Research topic: Methods Paper

Tue May 07 00:00:00 EDT 2013

Sequence and expression analysis of gaps in human chromosome 20.

Nucleic acids research

Minocherhomji S,Seemann S,Mang Y,El-Schich Z,Bak M,Hansen C,Papadopoulos N,Josefsen K,Nielsen H,Gorodkin J,Tommerup N,Silahtaroglu A

Wed Aug 01 00:00:00 EDT 2012

Enhancer-associated H3K4 monomethylation by Trithorax-related, the Drosophila homolog of mammalian Mll3/Mll4.

Genes & development

Herz HM,Mohan M,Garruss AS,Liang K,Takahashi YH,Mickey K,Voets O,Verrijzer CP,Shilatifard A

Research topic: Transcription Regulation

Sat Dec 01 00:00:00 EST 2012

Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells.

Journal of virology

Murata T,Kondo Y,Sugimoto A,Kawashima D,Saito S,Isomura H,Kanda T,Tsurumi T

Research topic: Virology (EBV)

Tue May 01 00:00:00 EDT 2012

Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines.

Experimental hematology

Valdez BC,Nieto Y,Murray D,Li Y,Wang G,Champlin RE,Andersson BS

Research topic: Lymphoma, stem cells

Mon Oct 01 00:00:00 EDT 2012

Synergistic cytotoxicity of the DNA alkylating agent busulfan, nucleoside analogs and suberoylanilide hydroxamic acid in lymphoma cell lines.

Leukemia & lymphoma

Valdez BC,Murray D,Nieto Y,Li Y,Wang G,Champlin RE,Andersson BS

Research topic: Cancer, Lymphoma

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K27; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3k27; H3Lys27me3; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K27me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K27; Tri-Methyl-H3 Lys27; Tri-Methyl-Histone H3 K27; Tri-Methyl-Histone H3 Lys27; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Mouse) Q8CGN9

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion histone

Process(es)

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Histone H3K27me3 Polyclonal Antibody

Histone H3K27me3 Antibody (39155) in ICC/IF

Product Details

39155

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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