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Human IgE Antibody (MA5-49599) in ELISA
ELISA analysis of Human IgE, microtiter wells were coated with Human IgE Monoclonal Antibody (4C3) (Product # MA5-49599) at 3 µg/mL. Human IgE was used as the antigen. Peroxidase conjugated mouse anti-human IgE monoclonal antibody (1A2) was used as the detection antibody. Result: 4C3 and 1A2 can be used as a matched antibody pair to detect and quantify the concentration of human IgE.
Product Details
MA5-49599
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4C3
Immunogen
Recombinant Human IgE
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3090634
Product Specific Information
This product was used in both Indirect and Sandwich ELISA.
This product does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgA and IgM.
Target Information
IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Immunoglobulin E; ImmunoglobulinE
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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