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Human IgM Antibody (PA5-121216) in WB
Western blot analysis of Human IgM in extracts of Raji cells. Samples were incubated with Human IgM Polyclonal antibody (Product # PA5-121216) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121216
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 213-453 of human IGHM (P01871)
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914788
Product Specific Information
Positive test controls include: Raji. The target is usually found in the following locations: Cell membrane, Secreted, Single-pass type I membrane protein.
Immunogen sequence: MCVPDQDTAI RVFAIPPSFA SIFLTKSTKL TCLVTDLTTY DSVTISWTRQ NGEAVKTHTN ISESHPNATF SAVGEASICE DDWNSGERFT CTVTHTDLPS PLKQTISRPK GVALHRPDVY LLPPAREQLN LRESATITCL VTGFSPADVF VQWMQRGQPL SPEKYVTSAP MPEPQAPGRY FAHSILTVSE EEWNTGETYT CVVAHEALPN RVTERTVDKS TGKPTLYNVS LVMSDTAGTC Y
Target Information
IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene Aliases: AGM1; MU; VH
Entrez Gene ID: (Human) 3507
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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